SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - engaged class
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rdy
Q-score: 0.472
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Template rna (17 kDa, RNA from synthetic construct)
- Sars-cov-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-rtc - engaged class (Complex from Severe acute respiratory syndrome coronavirus 2)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chapso (631 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - swiveled class
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7re0
Q-score: 0.323
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Template rna (17 kDa, RNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-rtc - swiveled class (Complex from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC (composite)
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 7re1
Q-score: 0.5
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Sars-cov-2 replication/transcription complex bound to nsp13 helicase - nsp13(2)-rtc (composite) (Complex from Severe acute respiratory syndrome coronavirus 2)
- Template rna (17 kDa, RNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chapso (631 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(1)-RTC
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 7re2
Q-score: 0.464
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Sars-cov-2 replication/transcription complex bound to nsp13 helicase - nsp13(1)-rtc (Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Template rna (17 kDa, RNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chapso (631 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - open class
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rdx
Q-score: 0.442
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Template rna (17 kDa, RNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chapso (631 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 replication/transcription complex bound to nsp13 helicase - nsp13(2)-rtc - open class (Complex from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - apo class
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7rdz
Q-score: 0.31
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Template rna (17 kDa, RNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 replication/transcription complex bound to nsp13 helicase - nsp13(2)-rtc - apo class (Complex from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC dimer
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7re3
Q-score: 0.321
Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA
Nat Struct Mol Biol (2022) 29 pp. 250-260 [ Pubmed: 35260847 DOI: doi:10.1038/s41594-022-00734-6 ]
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Template rna (17 kDa, RNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chapso (631 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Product rna (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 replication/transcription complex bound to nsp13 helicase - nsp13(2)-rtc dimer (Complex from Severe acute respiratory syndrome coronavirus 2)