I-F3b Cascade-TniQ full R-loop complex
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 7u5d
Q-score: 0.457
Park JU, Petassi MT, Hsieh SC, Mehrotra E, Schuler G, Budhathoki J, Truong VH, Thyme SB, Ke A, Kellogg EH, Peters JE
Mol Cell (2023) 83 pp. 1827-1838.e6 [ Pubmed: 37267904 DOI: doi:10.1016/j.molcel.2023.05.005 ]
- Cas8/5 (78 kDa, Protein from Aeromonas salmonicida)
- Cas7 (39 kDa, Protein from Aeromonas salmonicida)
- Non-target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- Target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- I-f3b cascade-tniq full r-loop complex (450 kDa, Complex from Aeromonas salmonicida)
- Tniq (46 kDa, Protein from Aeromonas salmonicida)
- Cas6 (23 kDa, Protein from Aeromonas salmonicida)
- Crrna (19 kDa, RNA from Aeromonas salmonicida)
I-F3b Cascade-TniQ full R-loop complex
Resolution: 4.03 Å
EM Method: Single-particle
Fitted PDBs: 7u5e
Q-score: 0.351
Park JU, Petassi MT, Hsieh SC, Mehrotra E, Schuler G, Budhathoki J, Truong VH, Thyme SB, Ke A, Kellogg EH, Peters JE
Mol Cell (2023) 83 pp. 1827-1838.e6 [ Pubmed: 37267904 DOI: doi:10.1016/j.molcel.2023.05.005 ]
- Cas8/5 (78 kDa, Protein from Aeromonas salmonicida)
- Cas7 (39 kDa, Protein from Aeromonas salmonicida)
- Non-target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- Target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- I-f3b cascade-tniq full r-loop complex (450 kDa, Complex from Aeromonas salmonicida)
- Tniq (46 kDa, Protein from Aeromonas salmonicida)
- Cas6 (23 kDa, Protein from Aeromonas salmonicida)
- Crrna (19 kDa, RNA from Aeromonas salmonicida)
Cryo-electron microscopy structure of TnsC(1-503)A225V bound to DNA
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 7mcs
Q-score: 0.439
Shen Y, Gomez-Blanco J, Petassi MT, Peters JE, Ortega J, Guarne A
Nat Struct Mol Biol (2022) 29 pp. 143-151 [ Pubmed: 35173349 DOI: doi:10.1038/s41594-022-00724-8 ]
- Dna (5'-d(p*gp*cp*gp*cp*gp*cp*gp*cp*gp*cp*gp*cp*gp*cp*g)-3') (4 kDa, DNA from synthetic construct)
- Dna (5'-d(p*cp*gp*cp*gp*cp*gp*cp*gp*cp*gp*cp*gp*cp*gp*c)-3') (4 kDa, DNA from synthetic construct)
- Complex of tnsc bound to dna in the presence of amppnp. (30 kDa, Complex from Escherichia coli)
- Transposon tn7 transposition protein tnsc (59 kDa, Protein from Escherichia coli)
- Tnsc(1-503)a225v bound to dna (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
ATPgS bound TnsC filament from shCAST system
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m99
Q-score: 0.477
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) 373 pp. 768-774 [ Pubmed: 34385391 DOI: doi:10.1126/science.abi8976 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Dna (5'-d(p*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*a)-3') (6 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (6 kDa, DNA from synthetic construct)
- Helical filament of tnsc monomers bound to dna (45 MDa, Complex from Scytonema hofmannii)
ADP-AlF3 bound TnsC structure from ShCAST system
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7m9a
Q-score: 0.342
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) 373 pp. 768-774 [ Pubmed: 34385391 DOI: doi:10.1126/science.abi8976 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Head-to-head dimer of tnsc hexamers bound to dna (400 kDa, Complex from Scytonema hofmannii)
- Dna (27-mer) (8 kDa, DNA from synthetic construct)
- Dna (27-mer) (8 kDa, DNA from synthetic construct)
ADP-AlF3 bound TnsC structure in closed form
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7m9b
Q-score: 0.317
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) 373 pp. 768-774 [ Pubmed: 34385391 DOI: doi:10.1126/science.abi8976 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Head to head dimer of tnsc hexamers bound to dna (400 kDa, Complex from Scytonema hofmannii)
- Dna (27-mer) (8 kDa, DNA from synthetic construct)
- Dna (27-mer) (8 kDa, DNA from synthetic construct)
ADP-AlF3 bound TnsC structure in open form
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7m9c
Q-score: 0.286
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) 373 pp. 768-774 [ Pubmed: 34385391 DOI: doi:10.1126/science.abi8976 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (34-mer) (10 kDa, DNA from synthetic construct)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Dna (34-mer) (10 kDa, DNA from synthetic construct)
- Head to head dimer of tnsc hexameters bound to dna (400 kDa, Complex from Scytonema hofmannii)
ATP bound TnsC filament structure from ShCAST system
Park J, Tsai A, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) [ DOI: doi:10.1126/science.abi8976 ]
- Tnsc (Protein from Scytonema hofmannii)
- Helical filament of tnsc monomers bound to dna (45 MDa, Complex from Scytonema hofmannii)
Head-to-head filament of TnsC from ShCAST system
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) 373 pp. 768-774 [ Pubmed: 34385391 DOI: doi:10.1126/science.abi8976 ]
- Head-to-head filament of tnsc on double-stranded dna (45 MDa, Complex from Scytonema hofmannii)
- Tnsc (Protein from Scytonema hofmannii)
ATP-bound TnsC-TniQ complex from ShCAST system
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7n6i
Q-score: 0.299
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science (2021) 373 pp. 768-774 [ Pubmed: 34385391 DOI: doi:10.1126/science.abi8976 ]
- Tnsc (Protein from Scytonema hofmannii)
- Helical filament of tnsc monomers bound to dna and tniq (400 kDa, Complex from Scytonema hofmannii)
- Tniq (Protein from Scytonema hofmannii)