CryoEM structure of TnsC(1-503) bound to TnsD(1-318) from E.coli Tn7
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 8glu
Q-score: 0.404
Shen Y, Krishnan SS, Petassi MT, Hancock MA, Peters JE, Guarne A
Mol Cell (2024) 84 pp. 2368-2381.e6 [ Pubmed: 38834067 DOI: doi:10.1016/j.molcel.2024.05.012 ]
- Transposon tn7 transposition protein tnsc (59 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transposon tn7 transposition protein tnsd (36 kDa, Protein from Escherichia coli)
- Complex of tnsc(1-503) bound to tnsd(1-318) with cofactors atp and adp (220 kDa, Complex from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Resolution: 3.51 Å
EM Method: Single-particle
Fitted PDBs: 8glw
Q-score: 0.399
Shen Y, Krishnan SS, Petassi MT, Hancock MA, Peters JE, Guarne A
Mol Cell (2024) 84 pp. 2368-2381.e6 [ Pubmed: 38834067 DOI: doi:10.1016/j.molcel.2024.05.012 ]
- Complex of tnsc(1-503) bound to tnsd(1-318) and dna with cofactors atp and adp (550 kDa, Complex from Escherichia coli)
- Transposon tn7 transposition protein tnsc (59 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transposon tn7 transposition protein tnsd (36 kDa, Protein from Escherichia coli)
- Dna (50-mer) (15 kDa, DNA from Escherichia coli)
- Dna (50-mer) (15 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 3.88 Å
EM Method: Single-particle
Fitted PDBs: 8glx
Q-score: 0.327
Shen Y, Krishnan SS, Petassi MT, Hancock MA, Peters JE, Guarne A
Mol Cell (2024) 84 pp. 2368-2381.e6 [ Pubmed: 38834067 DOI: doi:10.1016/j.molcel.2024.05.012 ]
- Dna (50-mer) (15 kDa, DNA from Escherichia coli)
- Tnsd(1-318) (Complex from Escherichia coli)
- Transposon tn7 transposition protein tnsc (59 kDa, Protein from Escherichia coli)
- Tnsc(1-503) (Complex from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of tnsc(1-503) bound to tnsd(1-318) and dna with cofactors atp and adp (500 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Transposon tn7 transposition protein tnsd (36 kDa, Protein from Escherichia coli)
- Dna (Complex from Escherichia coli)
- Dna (50-mer) (15 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8vcj
Q-score: 0.456
Shen Y, Krishnan SS, Petassi MT, Hancock MA, Peters JE, Guarne A
Mol Cell (2024) 84 pp. 2368-2381.e6 [ Pubmed: 38834067 DOI: doi:10.1016/j.molcel.2024.05.012 ]
- Transposon tn7 transposition protein tnsc (59 kDa, Protein from Escherichia coli)
- Dna (15 kDa, DNA from Escherichia coli)
- Complex of tnsc(1-503) bound to tnsd(1-318) and dna with cofactors atpgs and adp (550 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Transposon tn7 transposition protein tnsd (36 kDa, Protein from Escherichia coli)
- Dna (15 kDa, DNA from Escherichia coli)
- Dna (Complex from Escherichia coli)
- Tnsc(1-503) and tnsd(1-318) (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 8vct
Q-score: 0.396
Shen Y, Krishnan SS, Petassi MT, Hancock MA, Peters JE, Guarne A
Mol Cell (2024) 84 pp. 2368-2381.e6 [ Pubmed: 38834067 DOI: doi:10.1016/j.molcel.2024.05.012 ]
- Dna (50-mer) (15 kDa, DNA from Escherichia coli)
- Transposon tn7 transposition protein tnsc (59 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cyoem structure of the tnsc(1-503)-tnsd(1-318)-dna complex in a 6:2:1 stoichiometry from e. coli tn7 bound to atpgs and adp (Complex)
- Transposon tn7 transposition protein tnsd (36 kDa, Protein from Escherichia coli)
- Tnsc(1-503)-tnsd(1-318) (Complex from Escherichia coli)
- Dna (Complex from Escherichia coli)
- Dna (50-mer) (15 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
I-F3b Cascade-TniQ full R-loop complex
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 7u5d
Q-score: 0.457
Park JU, Petassi MT, Hsieh SC, Mehrotra E, Schuler G, Budhathoki J, Truong VH, Thyme SB, Ke A, Kellogg EH, Peters JE
Mol Cell (2023) 83 pp. 1827-1838.e6 [ Pubmed: 37267904 DOI: doi:10.1016/j.molcel.2023.05.005 ]
- Cas6 (23 kDa, Protein from Aeromonas salmonicida)
- I-f3b cascade-tniq full r-loop complex (450 kDa, Complex from Aeromonas salmonicida)
- Target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- Cas7 (39 kDa, Protein from Aeromonas salmonicida)
- Crrna (19 kDa, RNA from Aeromonas salmonicida)
- Cas8/5 (78 kDa, Protein from Aeromonas salmonicida)
- Non-target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- Tniq (46 kDa, Protein from Aeromonas salmonicida)
I-F3b Cascade-TniQ full R-loop complex
Resolution: 4.03 Å
EM Method: Single-particle
Fitted PDBs: 7u5e
Q-score: 0.351
Park JU, Petassi MT, Hsieh SC, Mehrotra E, Schuler G, Budhathoki J, Truong VH, Thyme SB, Ke A, Kellogg EH, Peters JE
Mol Cell (2023) 83 pp. 1827-1838.e6 [ Pubmed: 37267904 DOI: doi:10.1016/j.molcel.2023.05.005 ]
- Cas6 (23 kDa, Protein from Aeromonas salmonicida)
- I-f3b cascade-tniq full r-loop complex (450 kDa, Complex from Aeromonas salmonicida)
- Target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- Cas7 (39 kDa, Protein from Aeromonas salmonicida)
- Crrna (19 kDa, RNA from Aeromonas salmonicida)
- Cas8/5 (78 kDa, Protein from Aeromonas salmonicida)
- Non-target strand dna (35 kDa, DNA from Aeromonas salmonicida)
- Tniq (46 kDa, Protein from Aeromonas salmonicida)
Resolution: 3.54 Å
EM Method: Helical reconstruction
Fitted PDBs: 7svv
Q-score: 0.454
Park JU, Tsai AW, Chen TH, Peters JE, Kellogg EH
PNAS (2022) 119 pp. e2202590119-e2202590119 [ DOI: doi:10.1073/pnas.2202590119 Pubmed: 35914146 ]
- Tnsc filament (31 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Amppnp-bound tnsbctd-tnsc filament from shcast element (400 kDa, Complex from [Scytonema hofmanni] UTEX 2349)
- Dna (5'-d(p*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*a)-3') (6 kDa, DNA from synthetic construct)
- Tnsbctd (1 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (5 kDa, DNA from synthetic construct)
Strand-transfer complex of TnsB from ShCAST
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 7svw
Q-score: 0.385
Park JU, Tsai AW, Chen TH, Peters JE, Kellogg EH
PNAS (2022) 119 pp. e2202590119-e2202590119 [ DOI: doi:10.1073/pnas.2202590119 Pubmed: 35914146 ]
- Tnsb (66 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Stc_le_rev1 (13 kDa, DNA from synthetic construct)
- Stc_le_for (21 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Strand-transfer complex of tnsb from shcast element (350 kDa, Complex from [Scytonema hofmanni] UTEX 2349)
- Stc_le_rev2 (6 kDa, DNA from synthetic construct)
Park JU, Tsai AW, Chen TH, Peters JE, Kellogg EH
PNAS (2022) 119 pp. e2202590119-e2202590119 [ DOI: doi:10.1073/pnas.2202590119 Pubmed: 35914146 ]