Pintscher S, Pietras R, Mielecki B, Szwalec M, Wójcik-Augustyn A, Indyka P, Rawski M, Koziej Ł, Jaciuk M, Ważny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ DOI: 10.1038/s41477-024-01804-x Pubmed: 39362993 ]
Image sets:
Single particle cryoEM data of spinach cytochrome b6f incubated with decylplastoquinone
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat. Plants. (2024) 10 pp. 1814-1825 [ DOI: 10.1038/s41477-024-01804-x ]
Image sets:
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 9es7
Q-score: 0.682
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ Pubmed: 39362993 DOI: doi:10.1038/s41477-024-01804-x ]
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from Spinacia oleracea)
- Undecyl-maltoside (496 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6 (24 kDa, Protein from Spinacia oleracea)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from Spinacia oleracea)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from Spinacia oleracea)
- Heme c (618 Da, Ligand)
- Water (18 Da, Ligand)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinacia oleracea)
- Beta-carotene (536 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinacia oleracea)
- Cytochrome f (35 kDa, Protein from Spinacia oleracea)
- Cytochrome b6f complex incubated with decylplastoquinone (220 kDa, Complex from Spinacia oleracea)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Resolution: 2.24 Å
EM Method: Single-particle
Fitted PDBs: 9es8
Q-score: 0.626
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ Pubmed: 39362993 DOI: doi:10.1038/s41477-024-01804-x ]
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from Spinacia oleracea)
- Undecyl-maltoside (496 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6 (24 kDa, Protein from Spinacia oleracea)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from Spinacia oleracea)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from Spinacia oleracea)
- Heme c (618 Da, Ligand)
- Water (18 Da, Ligand)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinacia oleracea)
- Beta-carotene (536 Da, Ligand)
- Decylplastoquinone (276 Da, Ligand)
- Cytochrome b6f complex with decylplastoquinol bound at quinone reduction site (220 kDa, Complex from Spinacia oleracea)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinacia oleracea)
- Cytochrome f (35 kDa, Protein from Spinacia oleracea)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 9es9
Q-score: 0.618
Pintscher S, Pietras R, Mielecki B, Szwalec M, Wojcik-Augustyn A, Indyka P, Rawski M, Koziej L, Jaciuk M, Wazny G, Glatt S, Osyczka A
Nat Plants (2024) 10 pp. 1814-1825 [ Pubmed: 39362993 DOI: doi:10.1038/s41477-024-01804-x ]
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from Spinacia oleracea)
- Undecyl-maltoside (496 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6 (24 kDa, Protein from Spinacia oleracea)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from Spinacia oleracea)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Heme c (618 Da, Ligand)
- Water (18 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinacia oleracea)
- Beta-carotene (536 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Cytochrome b6f complex inhibited with dbmib (2,5-dibromo-3-isopropyl-6-methylbenzo-1,4-quinone) (220 kDa, Complex from Spinacia oleracea)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinacia oleracea)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinacia oleracea)
- Cytochrome f (35 kDa, Protein from Spinacia oleracea)
- 2,5-dibromo-3-isopropyl-6-methylbenzo-1,4-quinone (321 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Spinacia oleracea endogenous cytochrome b6f complex
Sarewicz M, Szwalec M, Pintscher S, Indyka P, Rawski M, Pietras R, Mielecki B, Koziej Ł, Jaciuk M, Glatt S, Osyczka A
Sci Adv (2023) 9 [ Pubmed: 36638176 DOI: 10.1126/sciadv.add9688 ]
Image sets:
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qrm
Q-score: 0.617
Sarewicz M, Szwalec M, Pintscher S, Indyka P, Rawski M, Pietras R, Mielecki B, Koziej L, Jaciuk M, Glatt S, Osyczka A
Sci Adv (2023) 9 pp. eadd9688-eadd9688 [ Pubmed: 36638176 DOI: doi:10.1126/sciadv.add9688 ]
- Cytochrome f (35 kDa, Protein from spinach)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from spinach)
- Cytochrome b6 (24 kDa, Protein from spinach)
- Beta-carotene (536 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from spinach)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from spinach)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Heme c (618 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from spinach)
- Cytochrome b6f complex (220 kDa, Complex from Spinacia oleracea)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from spinach)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (3 kDa, Protein from spinach)
Resolution: 2.13 Å
EM Method: Single-particle
Fitted PDBs: 7zyv
Q-score: 0.644
Sarewicz M, Szwalec M, Pintscher S, Indyka P, Rawski M, Pietras R, Mielecki B, Koziej L, Jaciuk M, Glatt S, Osyczka A
Sci Adv (2023) 9 pp. eadd9688-eadd9688 [ Pubmed: 36638176 DOI: doi:10.1126/sciadv.add9688 ]
- Cytochrome f (35 kDa, Protein from spinach)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (24 kDa, Protein from spinach)
- Cytochrome b6 (24 kDa, Protein from spinach)
- Beta-carotene (536 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Undecyl-maltoside (496 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from spinach)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from spinach)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Heme c (618 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from spinach)
- Cytochrome b6f complex (220 kDa, Complex from Spinacia oleracea)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Thylakoid soluble phosphoprotein (10 kDa, Protein from spinach)
- Chlorophyll a (893 Da, Ligand)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from spinach)
- Cytochrome b6-f complex subunit 7 (12 kDa, Protein from spinach)