Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7txd
Q-score: 0.352
Glogl M, Friedrich N, Cerutti G, Lemmin T, Kwon YD, Gorman J, Maliqi L, Mittl PRE, Hesselman MC, Schmidt D, Weber J, Foulkes C, Dingens AS, Bylund T, Olia AS, Verardi R, Reinberg T, Baumann NS, Rusert P, Dreier B, Shapiro L, Kwong PD, Pluckthun A, Trkola A
Nat Struct Mol Biol (2023) 30 pp. 1323-1336 [ DOI: doi:10.1038/s41594-023-01062-z Pubmed: 37605043 ]
- Broadly neutralizing darpin bnd.9 (21 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- T-cell surface glycoprotein cd4 (20 kDa, Protein from Homo sapiens)
- Bg505 sosip hiv-1 env trimer in complex with cd4 receptor (d1d2) and broadly neutralizing darpin bnd.9 (Complex from Human immunodeficiency virus 1)
Human Neurokinin 1 receptor (NK1R) substance P Gq chimera (mGsqi) complex
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 7p00
Q-score: 0.546
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Substance p (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gq chimera (gsqi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Human Neurokinin 1 receptor (NK1R) substance P Gs complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 7p02
Q-score: 0.502
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Protachykinin-1 (1 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gs chimera (gsi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Generation of ordered protein assemblies using rigid three-body fusion
Vulovic I, Yao Q, Park YJ, Courbet A, Norris A, Busch F, Sahasrabuddhe A, Merten H, Sahtoe DD, Ueda G, Fallas JA, Weaver SJ, Hsia Y, Langan RA, Pluckthun A, Wysocki VH, Veesler D, Jensen GJ, Baker D
PNAS (2021) 118 [ Pubmed: 34074752 DOI: doi:10.1073/pnas.2015037118 ]
- D3-19.19 (Protein from synthetic construct)
- D3-19.19 (Complex)
- D3-19.19 (Complex from synthetic construct)
Vulovic I, Yao Q, Park YJ, Courbet A, Norris A, Busch F, Sahasrabuddhe A, Merten H, Sahtoe DD, Ueda G, Fallas JA, Weaver SJ, Hsia Y, Langan RA, Pluckthun A, Wysocki VH, Veesler D, Jensen GJ, Baker D
PNAS (2021) 118 [ Pubmed: 34074752 DOI: doi:10.1073/pnas.2015037118 ]
- D3-19.14 (Protein from synthetic construct)
- D3-19.14 (Complex from synthetic construct)
Vulovic I, Yao Q, Park YJ, Courbet A, Norris A, Busch F, Sahasrabuddhe A, Merten H, Sahtoe DD, Ueda G, Fallas JA, Weaver SJ, Hsia Y, Langan RA, Pluckthun A, Wysocki VH, Veesler D, Jensen GJ, Baker D
PNAS (2021) 118 [ Pubmed: 34074752 DOI: doi:10.1073/pnas.2015037118 ]
- D2-1.4h (Complex from synthetic construct)
- D2-1.4h (Protein from synthetic construct)
