Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7txd
Q-score: 0.352
Glogl M, Friedrich N, Cerutti G, Lemmin T, Kwon YD, Gorman J, Maliqi L, Mittl PRE, Hesselman MC, Schmidt D, Weber J, Foulkes C, Dingens AS, Bylund T, Olia AS, Verardi R, Reinberg T, Baumann NS, Rusert P, Dreier B, Shapiro L, Kwong PD, Pluckthun A, Trkola A
Nat Struct Mol Biol (2023) 30 pp. 1323-1336 [ DOI: doi:10.1038/s41594-023-01062-z Pubmed: 37605043 ]
- Broadly neutralizing darpin bnd.9 (21 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- T-cell surface glycoprotein cd4 (20 kDa, Protein from Homo sapiens)
- Bg505 sosip hiv-1 env trimer in complex with cd4 receptor (d1d2) and broadly neutralizing darpin bnd.9 (Complex from Human immunodeficiency virus 1)
Human Oxytocin receptor (OTR) oxytocin Gq chimera (mGoqi) complex
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7qvm
Q-score: 0.423
Waltenspuhl Y, Ehrenmann J, Vacca S, Thom C, Medalia O, Pluckthun A
Nat Commun (2022) 13 pp. 4153-4153 [ DOI: doi:10.1038/s41467-022-31325-0 Pubmed: 35851571 ]
- Antibody fragment scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha,cdna flj31446 fis, clone nt2ne2000909, highly similar to guanine nucleotide-binding protein g(o) subunit alpha 1 (25 kDa, Protein from Homo sapiens)
- Oxytocin receptor (39 kDa, Protein from Homo sapiens)
- Oxytocin (Complex from Homo sapiens)
- Antibody fragment scfv16 (Complex from Mus musculus)
- Otr in complex with oxytocin, heterotrimeric mini-gq chimera (goqi) and scfv16 (Complex)
- Oxytocin (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Heterotrimeric mini-gq chimera (goqi) and oxytocin receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7pdf
Q-score: 0.445
Qi C, Lavriha P, Mehta V, Khanppnavar B, Mohammed I, Li Y, Lazaratos M, Schaefer JV, Dreier B, Pluckthun A, Bondar AN, Dessauer CW, Korkhov VM
Nat Commun (2022) 13 pp. 1045-1045 [ DOI: doi:10.1038/s41467-022-28685-y Pubmed: 35210418 ]
- Adenylyl cyclase 9 (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (47 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Adenylyl cyclase 9 bound to mant-gtp (146 MDa, Complex)
- Adenylate cyclase 9 (151 kDa, Protein from Bos taurus)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Structure of Adenylyl cyclase 9 in complex with Gs protein alpha subunit and MANT-GTP
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7pde
Q-score: 0.288
Qi C, Lavriha P, Mehta V, Khanppnavar B, Mohammed I, Li Y, Lazaratos M, Schaefer JV, Dreier B, Pluckthun A, Bondar AN, Dessauer CW, Korkhov VM
Nat Commun (2022) 13 pp. 1045-1045 [ DOI: doi:10.1038/s41467-022-28685-y Pubmed: 35210418 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Bos taurus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (47 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Adenylyl cyclase 9 bound to mant-gtp (146 MDa, Complex)
- Adenylate cyclase 9 (151 kDa, Protein from Bos taurus)
- Adenylate cyclase 9 (Complex from Bos taurus)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Human Neurokinin 1 receptor (NK1R) substance P Gq chimera (mGsqi) complex
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 7p00
Q-score: 0.546
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Substance p (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gq chimera (gsqi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Human Neurokinin 1 receptor (NK1R) substance P Gs complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 7p02
Q-score: 0.502
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Protachykinin-1 (1 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gs chimera (gsi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Structure of NTS-NTSR1-Gi complex in lipid nanodisc, canonical state, AHD and nanodisc mask out
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7l0p
Q-score: 0.404
Zhang M, Gui M, Wang ZF, Gorgulla C, Yu JJ, Wu H, Sun ZJ, Klenk C, Merklinger L, Morstein L, Hagn F, Pluckthun A, Brown A, Nasr ML, Wagner G
Nat Struct Mol Biol (2021) 28 pp. 258-267 [ Pubmed: 33633398 DOI: doi:10.1038/s41594-020-00554-6 ]
- Nts-ntsr1-gi complex in lipid nanodisc (10 kDa, Complex)
- Nts (Complex from Rattus norvegicus)
- Neurotensin (1 kDa, Protein from Rattus norvegicus)
- G(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit gamma-t1 (9 kDa, Protein from Homo sapiens)
- G(i) subunit alpha-1 (Complex from Homo sapiens)
- Ntsr1 (Complex from Rattus norvegicus)
- G(t) subunit gamma-1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Structure of NTS-NTSR1-Gi complex in lipid nanodisc, canonical state, overall
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7l0q
Q-score: 0.325
Zhang M, Gui M, Wang ZF, Gorgulla C, Yu JJ, Wu H, Sun ZJ, Klenk C, Merklinger L, Morstein L, Hagn F, Pluckthun A, Brown A, Nasr ML, Wagner G
Nat Struct Mol Biol (2021) 28 pp. 258-267 [ Pubmed: 33633398 DOI: doi:10.1038/s41594-020-00554-6 ]
- Nts-ntsr1-gi complex in lipid nanodisc (10 kDa, Complex)
- Nts (Complex from Rattus norvegicus)
- Neurotensin (1 kDa, Protein from Rattus norvegicus)
- G(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit gamma-t1 (9 kDa, Protein from Homo sapiens)
- G(i) subunit alpha-1 (Complex from Homo sapiens)
- Ntsr1 (Complex from Rattus norvegicus)
- G(t) subunit gamma-1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Structure of NTS-NTSR1-Gi complex in lipid nanodisc, noncanonical state, AHD and nanodisc mask out
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7l0r
Q-score: 0.311
Zhang M, Gui M, Wang ZF, Gorgulla C, Yu JJ, Wu H, Sun ZJ, Klenk C, Merklinger L, Morstein L, Hagn F, Pluckthun A, Brown A, Nasr ML, Wagner G
Nat Struct Mol Biol (2021) 28 pp. 258-267 [ Pubmed: 33633398 DOI: doi:10.1038/s41594-020-00554-6 ]
- Nts-ntsr1-gi complex in lipid nanodisc (10 kDa, Complex)
- Nts (Complex from Rattus norvegicus)
- Neurotensin (1 kDa, Protein from Rattus norvegicus)
- G(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit gamma-t1 (9 kDa, Protein from Homo sapiens)
- G(i) subunit alpha-1 (Complex from Homo sapiens)
- Ntsr1 (Complex from Rattus norvegicus)
- G(t) subunit gamma-1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Structure of NTS-NTSR1-Gi complex in lipid nanodisc, noncanonical state, overall
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7l0s
Q-score: 0.278
Zhang M, Gui M, Wang ZF, Gorgulla C, Yu JJ, Wu H, Sun ZJ, Klenk C, Merklinger L, Morstein L, Hagn F, Pluckthun A, Brown A, Nasr ML, Wagner G
Nat Struct Mol Biol (2021) 28 pp. 258-267 [ Pubmed: 33633398 DOI: doi:10.1038/s41594-020-00554-6 ]
- Nts-ntsr1-gi complex in lipid nanodisc (10 kDa, Complex)
- Nts (Complex from Rattus norvegicus)
- Neurotensin (1 kDa, Protein from Rattus norvegicus)
- G(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(t) subunit gamma-t1 (9 kDa, Protein from Homo sapiens)
- G(i) subunit alpha-1 (Complex from Homo sapiens)
- Ntsr1 (Complex from Rattus norvegicus)
- G(t) subunit gamma-1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)