Xu X, Godoy-Ruiz R, Adipietro KA, Peralta C, Ben-Hail D, Varney KM, Cook ME, Roth BM, Wilder PT, Cleveland T, Grishaev A, Neu HM, Michel SLJ, Yu W, Beckett D, Rustandi RR, Lancaster C, Loughney JW, Kristopeit A, Christanti S, Olson JW, MacKerell AD, Georges AD, Pozharski E, Weber DJ
Proc Natl Acad Sci U S A (2020) 117 pp. 1049-1058 [ Pubmed: 31896582 DOI: 10.1073/pnas.1919490117 ]
Image sets:
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8g6u
Q-score: 0.433
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ Pubmed: 37872202 DOI: doi:10.1038/s41467-023-42500-2 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Light chain of 8anc195 (23 kDa, Protein from Homo sapiens)
- Cryo-em structure of t/f100 sosip.664 hiv-1 env trimer with lmhs mutations in complex with 8anc195 and 10-1074 (6 MDa, Complex from Human immunodeficiency virus 1)
- Crf-1_ae t/f100 hiv-1 gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Heavy chain of 10-1074 (25 kDa, Protein from Homo sapiens)
- Light chain of 10-1074 (23 kDa, Protein from Homo sapiens)
- Heavy chain of 8anc195 (25 kDa, Protein from Homo sapiens)
- Crf01_ae t/f100 hiv-1 gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
Cryo-EM structure of BG505 SOSIP.664 HIV-1 Env trimer in complex with temsavir, 8ANC195, and 10-1074
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8ttw
Q-score: 0.507
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ Pubmed: 37872202 DOI: doi:10.1038/s41467-023-42500-2 ]
- Envelope glycoprotein gp160 (54 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Antibody 10-1074 heavy chain (25 kDa, Protein from Homo sapiens)
- Antibody 8anc195 light chain (23 kDa, Protein from Homo sapiens)
- 1-[4-(benzenecarbonyl)piperazin-1-yl]-2-[4-methoxy-7-(3-methyl-1h-1,2,4-triazol-1-yl)-1h-pyrrolo[2,3-c]pyridin-3-yl]ethane-1,2-dione (473 Da, Ligand)
- Complex of bg505 sosip.664 with temsavir and the antibody fabs of 8anc195 and 10-1074 (Complex from Homo sapiens)
- Antibody 8anc195 heavy chain (25 kDa, Protein from Homo sapiens)
- Antibody 10-1074 light chain (23 kDa, Protein from Homo sapiens)
- Bg505 sosip.664 transmembrane protein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8ekk
Q-score: 0.538
Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ
To Be Published
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8ekl
Q-score: 0.577
Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ
To Be Published
Clostridioides difficile binary toxin translocase CDTb double mutant - D623A D734A
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8ekm
Q-score: 0.502
Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ
To Be Published
Cryo-EM structure of T/F100 SOSIP.664 HIV-1 Env trimer in complex with 8ANC195 and 10-1074
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8dok
Q-score: 0.484
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ Pubmed: 37872202 DOI: doi:10.1038/s41467-023-42500-2 ]
- Cryo-em structure of t/f100 sosip.664 hiv-1 env trimer in complex with 8anc195 and 10-1074 (6 MDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Light chain of 8anc195 (23 kDa, Protein from Homo sapiens)
- Crf-1_ae t/f100 env gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Crf-1_ae t/f100 hiv-1 gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Heavy chain of 10-1074 (25 kDa, Protein from Homo sapiens)
- Light chain of 10-1074 (23 kDa, Protein from Homo sapiens)
- Heavy chain of 8anc195 (25 kDa, Protein from Homo sapiens)
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8fsj
Q-score: 0.368
Metcalf MC, Janus BM, Yin R, Wang R, Guest JD, Pozharski E, Law M, Mariuzza RA, Toth EA, Pierce BG, Fuerst TR, Ofek G
Nat Commun (2023) 14 pp. 3980-3980 [ DOI: doi:10.1038/s41467-023-39659-z Pubmed: 37407593 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hepc74 light chain (23 kDa, Protein from Homo sapiens)
- Hcv e1 ectodomain, synzip1 scaffold fusion (22 kDa, Protein from synthetic construct)
- Ar4a light chain (25 kDa, Protein from Homo sapiens)
- Ar4a heavy chain (31 kDa, Protein from Homo sapiens)
- Hcv e2 ectodomain, synzip2 scaffold fusion (42 kDa, Protein from synthetic construct)
- Hcv e1e2 ectodomain in complex with ar4a, hepc74, and igh520 (250 kDa, Complex from Hepacivirus C)
- Hepc74 heavy chain (27 kDa, Protein from Homo sapiens)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8czz
Q-score: 0.428
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ Pubmed: 37872202 DOI: doi:10.1038/s41467-023-42500-2 ]
- Heavy chain of 10-1074 fab (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Light chain of 8anc195 fab (23 kDa, Protein from Homo sapiens)
- Light chain of 10-1074 fab (23 kDa, Protein from Homo sapiens)
- Crf01_ae t/f100 hiv-1 gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Crf-1_ae t/f100 hiv-1 gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Cryo-em structure of t/f100 sosip.664 hiv-1 env trimer with lmhs mutations in complex with temsavir, 8anc195, and 10-1074 (6 MDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Heavy chain of 8anc195 fab (25 kDa, Protein from Homo sapiens)
- 1-[4-(benzenecarbonyl)piperazin-1-yl]-2-[4-methoxy-7-(3-methyl-1h-1,2,4-triazol-1-yl)-1h-pyrrolo[2,3-c]pyridin-3-yl]ethane-1,2-dione (473 Da, Ligand)
Resolution: 4.18 Å
EM Method: Single-particle
Fitted PDBs: 7rq6
Q-score: 0.33
Beaudoin-Bussieres G, Chen Y, Ullah I, Prevost J, Tolbert WD, Symmes K, Ding S, Benlarbi M, Gong SY, Tauzin A, Gasser R, Chatterjee D, Vezina D, Goyette G, Richard J, Zhou F, Stamatatos L, McGuire AT, Charest H, Roger M, Pozharski E, Kumar P, Mothes W, Uchil PD, Pazgier M, Finzi A
Cell Rep (2022) 38 pp. 110368-110368 [ DOI: doi:10.1016/j.celrep.2022.110368 Pubmed: 35123652 ]
- Cv3-13 fab light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cryo-em structure of sars-cov-2 spike in complex with non-neutralizing, ntd-directed human antibody cv3-13 (670 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (138 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Cv3-13 fab heavy chain (24 kDa, Protein from Homo sapiens)