Mycobacterium tuberculosis paused transcription complex with Bacillus subtilis NusG
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ehq
Q-score: 0.506
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Rna (29-mer) (9 kDa, RNA from synthetic construct)
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Paused transcription complex with nusg (Complex from Mycobacterium tuberculosis H37Rv)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
M. tuberculosis RNAP pause escaped complex with Bacillus subtilis NusG and GMPCPP
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8ej3
Q-score: 0.511
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Magnesium ion (24 Da, Ligand)
- Dna (29-mer) (12 kDa, DNA from synthetic construct)
- Rna (5'-r(p*up*cp*gp*gp*cp*ap*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (9 kDa, RNA from synthetic RNA)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Pause escaped transcription complex with nusg and gmpcpp (Complex from Mycobacterium tuberculosis H37Rv)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna (32-mer) (12 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
Mycobacterium tuberculosis transcription elongation complex with Bacillus subtilis NusG (EC_LG)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8eoe
Q-score: 0.475
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Rna (9 kDa, RNA from synthetic construct)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- M. tuberculosis transcription elongation complex with nusg (Complex from Mycobacterium tuberculosis H37Rv)
- Dna (35-mer) (12 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
Mycobacterium tuberculosis transcription elongation complex with Bacillus subtilis NusG (EC_PG)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8eof
Q-score: 0.467
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Rna (29-mer) (9 kDa, RNA from synthetic RNA)
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- M. tuberculosis transcription elongation complex with nusg (Complex from Mycobacterium tuberculosis H37Rv)
- Dna (35-mer) (12 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
M. tuberculosis RNAP elongation complex with NusG and CMPCPP
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8eos
Q-score: 0.502
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription elongation complex with m. tuberculosis nusg and cmpcpp (Complex from Mycobacterium tuberculosis H37Rv)
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Dna (36-mer) (12 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine (481 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Rna (5'-r(p*ap*ap*gp*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
M. tuberculosis RNAP elongation complex with NusG
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8eot
Q-score: 0.484
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Transcription elongation complex with m. tuberculosis nusg and cmpcpp (Complex from Mycobacterium tuberculosis H37Rv)
- Dna (31-mer) (12 kDa, DNA from synthetic construct)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna (33-mer) (12 kDa, DNA from synthetic construct)
- Rna (5'-r(p*ap*ap*gp*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
M. tuberculosis RNAP paused complex with B. subtilis NusG and GMPCPP
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8exy
Q-score: 0.457
Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS
PNAS (2023) 120 pp. e2218516120-e2218516120 [ DOI: doi:10.1073/pnas.2218516120 Pubmed: 36745813 ]
- Rna (29-mer) (9 kDa, RNA from synthetic construct)
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (38-mer) (12 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Pause escaped transcription complex with nusg and gmpcpp (Complex from Mycobacterium tuberculosis H37Rv)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)