Dimer structure of procaryotic Ago
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8k87
Q-score: 0.475
Sun D, Zhu K, Wang L, Mu Z, Wu K, Hua L, Qin B, Gao X, Wang Y, Cui S
Cell Rep (2024) 43 pp. 114391-114391 [ DOI: doi:10.1016/j.celrep.2024.114391 Pubmed: 38923459 ]
- Magnesium ion (24 Da, Ligand)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Rna/dna (21-mer) (6 kDa, Macromolecule from synthetic construct)
- Dna (41-mer) (12 kDa, DNA from synthetic construct)
- Dimmeric gssirt2/ago-gran-tdna complex (Complex from Geobacter sulfurreducens)
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8k88
Q-score: 0.548
Sun D, Zhu K, Wang L, Mu Z, Wu K, Hua L, Qin B, Gao X, Wang Y, Cui S
Cell Rep (2024) 43 pp. 114391-114391 [ DOI: doi:10.1016/j.celrep.2024.114391 Pubmed: 38923459 ]
- Magnesium ion (24 Da, Ligand)
- Dna/rna (21-mer) (6 kDa, Macromolecule from synthetic construct)
- Dna (41-mer) (12 kDa, DNA from synthetic construct)
- Short prokaryotic argonaute associated sir2 (120 MDa, Complex from Geobacter sulfurreducens)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA tetramer (NADase active form)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8it1
Q-score: 0.271
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
- The tetramer structural of prokaryotic argonaute with dna and rna complex (Complex from Thermoflavifilum thermophilum)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA monomer
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8isz
Q-score: 0.321
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- The dimer structural of prokaryotic argonaute with dna and rna complex (Complex from Thermoflavifilum thermophilum)
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-2)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8it0
Q-score: 0.397
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- The dimer of prokaryotic argonaute system with dna and rna (Complex from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-1)
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8k9g
Q-score: 0.404
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- The dimer of prokaryotic argonaute system with dna and rna (Complex from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)