Structure of CX3CL1-US28-G11iN18-scFv16 in TL-state
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7rkf
Q-score: 0.44
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Scfv16 (30 kDa, Complex from Mus musculus)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein subunit alpha-11 (41 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- G11in18 heterotrimer (90 kDa, Complex from Homo sapiens)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Cx3cl1-us28-g11in18-scfv16 complex (Complex)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Structure of CX3CL1-US28-Gi-scFv16 in C-state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rkm
Q-score: 0.477
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Cholesterol (386 Da, Ligand)
- Scfv16 (30 kDa, Complex from Mus musculus)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human betaherpesvirus 5)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Structure of CX3CL1-US28-Gi-scFv16 in OC-state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7rkn
Q-score: 0.447
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Structure of US27-Gi-scFv16 in CL-state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rkx
Q-score: 0.529
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- G-protein coupled receptor homolog us27 (44 kDa, Protein from Human cytomegalovirus)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Us27 (40 kDa, Complex from Human betaherpesvirus 5)
- Us27-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Binding mode of US27-Gi-scFv16 in OCL-state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7rky
Q-score: 0.43
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Us27-gi-scfv16 complex (Complex)
- Us27 (40 kDa, Complex from Human betaherpesvirus 5)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- G-protein coupled receptor homolog us27 (44 kDa, Protein from Human cytomegalovirus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Structure of the Epstein-Barr virus GPCR BILF1 in complex with human Gi
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7jhj
Q-score: 0.54
Tsutsumi N, Qu Q, Mavri M, Baggesen MS, Maeda S, Waghray D, Berg C, Kobilka BK, Rosenkilde MM, Skiniotis G, Garcia KC
Immunity (2021) 54 pp. 1405-1416.e7 [ Pubmed: 34216564 DOI: doi:10.1016/j.immuni.2021.06.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Bilf1 (40 kDa, Complex from Human gammaherpesvirus 4)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Bilf1 (35 kDa, Protein from Epstein-Barr virus)
- Bilf1-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gi heterotrimer (70 kDa, Complex from Homo sapiens)