Structure of CX3CL1-US28-G11iN18-scFv16 in TL-state
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7rkf
Q-score: 0.44
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Cx3cl1-us28-g11in18-scfv16 complex (Complex)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein subunit alpha-11 (41 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G11in18 heterotrimer (90 kDa, Complex from Homo sapiens)
Structure of CX3CL1-US28-Gi-scFv16 in C-state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rkm
Q-score: 0.477
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human betaherpesvirus 5)
Structure of CX3CL1-US28-Gi-scFv16 in OC-state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7rkn
Q-score: 0.447
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Structure of US27-Gi-scFv16 in CL-state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rkx
Q-score: 0.529
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Us27-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Us27 (40 kDa, Complex from Human betaherpesvirus 5)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- G-protein coupled receptor homolog us27 (44 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Binding mode of US27-Gi-scFv16 in OCL-state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7rky
Q-score: 0.43
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Us27-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Us27 (40 kDa, Complex from Human betaherpesvirus 5)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- G-protein coupled receptor homolog us27 (44 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)