CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t2g
Q-score: 0.672
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ Pubmed: 36411392 DOI: doi:10.1038/s41589-022-01208-y ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-opioid receptor / gi protein complex bound to mitragynine pseudoindoxyl (mp). (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,mu-type opioid receptor (55 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Mitragynine pseudoindoxyl (414 Da, Ligand)
CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7t2h
Q-score: 0.528
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ Pubmed: 36411392 DOI: doi:10.1038/s41589-022-01208-y ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
- Mu-opioid receptor - gi protein complex bound to lofentanil (lft) (Complex from Mus musculus)
- Lofentanil (408 Da, Ligand)
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 7wld
Q-score: 0.615
Xu Y, Jia G, Li T, Zhou Z, Luo Y, Chao Y, Bao J, Su Z, Qu Q, Li D
Nat Commun (2022) 13 pp. 2617-2617 [ Pubmed: 35551457 DOI: doi:10.1038/s41467-022-30250-6 ]
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Gpi transamidase component pig-t (94 kDa, Protein from Homo sapiens)
- Phosphatidylinositol glycan anchor biosynthesis class u protein (80 kDa, Protein from Homo sapiens)
- Gpi transamidase complex (Complex from Homo sapiens)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- 2-azanylethyl [(2~{s},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate (303 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Gpi transamidase component pig-s (90 kDa, Protein from Homo sapiens)
- Glycosylphosphatidylinositol anchor attachment 1 protein (96 kDa, Protein from Homo sapiens)
- Digitonin (1 kDa, Ligand)
- Gpi-anchor transamidase (73 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- 2-amino-2-deoxy-alpha-d-glucopyranose (179 Da, Ligand)
- [(2~{r})-1-hexadecanoyloxy-3-[[(1~{s},2~{r},3~{r},4~{s},5~{s},6~{r})-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-propan-2-yl] (9~{z},11~{z})-octadeca-9,11-dienoate (1 kDa, Ligand)
- (4s,7r)-7-[(hexadecanoyloxy)methyl]-4-hydroxy-n,n,n-trimethyl-4,9-dioxo-3,5,8-trioxa-4lambda~5~-phosphahexacosan-1-aminium (763 Da, Ligand)
Structure of CX3CL1-US28-Gi-scFv16 in C-state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7rkm
Q-score: 0.477
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Cholesterol (386 Da, Ligand)
- Scfv16 (30 kDa, Complex from Mus musculus)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human betaherpesvirus 5)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Structure of CX3CL1-US28-Gi-scFv16 in OC-state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7rkn
Q-score: 0.447
Tsutsumi N, Maeda S, Qu Q, Voegele M, Jude KM, Suomivuori CM, Panova O, Waghray D, Kato HE, Velasco A, Dror RO, Skiniotis G, Kobilka BK, Garcia KC
Sci Adv (2022) 8 pp. eabl5442- [ DOI: doi:10.1126/sciadv.abl5442 ]
- Gi heterotrimer (90 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cx3cl1-us28-gi-scfv16 complex (Complex)
- G-protein coupled receptor homolog us28 (42 kDa, Protein from Human cytomegalovirus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Fractalkine (10 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Cx3cl1-us28 (50 kDa, Complex from Human betaherpesvirus 5)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
CryoEM structure of SGLT1 at 3.4 A resolution
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7sl8
Q-score: 0.467
Han L, Qu Q, Aydin D, Panova O, Robertson MJ, Xu Y, Dror RO, Skiniotis G, Feng L
Nature (2022) 601 pp. 274-279 [ Pubmed: 34880492 DOI: doi:10.1038/s41586-021-04211-w ]
- Nanobody nb1 (14 kDa, Protein from synthetic construct)
- Sodium/glucose cotransporter 1 (74 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Nanobody-sglt complex (Complex from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7sl9
Q-score: 0.463
Han L, Qu Q, Aydin D, Panova O, Robertson MJ, Xu Y, Dror RO, Skiniotis G, Feng L
Nature (2022) 601 pp. 274-279 [ Pubmed: 34880492 DOI: doi:10.1038/s41586-021-04211-w ]
- Butanoic acid (88 Da, Ligand)
- Sodium-coupled monocarboxylate transporter 1 (67 kDa, Protein from Homo sapiens)
- Smct1 and nanobody complex (Complex from Homo sapiens)
- Nanobody nb2 (14 kDa, Protein from synthetic construct)
Structure of the Epstein-Barr virus GPCR BILF1 in complex with human Gi
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7jhj
Q-score: 0.54
Tsutsumi N, Qu Q, Mavri M, Baggesen MS, Maeda S, Waghray D, Berg C, Kobilka BK, Rosenkilde MM, Skiniotis G, Garcia KC
Immunity (2021) 54 pp. 1405-1416.e7 [ Pubmed: 34216564 DOI: doi:10.1016/j.immuni.2021.06.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Bilf1 (40 kDa, Complex from Human gammaherpesvirus 4)
- Antibody fragment scfv16 (27 kDa, Protein from Mus musculus)
- Scfv16 (30 kDa, Complex from Mus musculus)
- Bilf1 (35 kDa, Protein from Epstein-Barr virus)
- Bilf1-gi-scfv16 complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gi heterotrimer (70 kDa, Complex from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6vwx
Q-score: 0.521
Gao S, Valinsky WC, On NC, Houlihan PR, Qu Q, Liu L, Pan X, Clapham DE, Yan N
PNAS (2020) 117 pp. 14187-14193 [ Pubmed: 32513729 DOI: doi:10.1073/pnas.1922903117 ]
- Sodium ion (22 Da, Ligand)
- Nachbac (Complex from Bacillus halodurans C-125)
- Bh1501 protein (31 kDa, Protein from Bacillus halodurans C-125)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6vx3
Q-score: 0.463
Gao S, Valinsky WC, On NC, Houlihan PR, Qu Q, Liu L, Pan X, Clapham DE, Yan N
PNAS (2020) 117 pp. 14187-14193 [ Pubmed: 32513729 DOI: doi:10.1073/pnas.1922903117 ]