Cholinephosphotransferase in complex with diacylglycerol in nanodisc
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody24 (Complex from Lama glama)
- Cholinephosphotransferase 1 (Protein from Saccharomyces cerevisiae S288C)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- Saccharomyces cerevisiae cdp-choline phosphotransferase in complex with diacyl-glycerol (Complex from Saccharomyces cerevisiae)
Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8urp
Q-score: 0.589
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody25 (Complex from Lama glama)
- Cholinephosphotransferase 1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- [2-cytidylate-o'-phosphonyloxyl]-ethyl-trimethyl-ammonium (488 Da, Ligand)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phoshocholine (734 Da, Ligand)
Cholinephosphotransferase in complex with selective inhibitor chelerythrine
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8urt
Q-score: 0.546
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody25 (Complex from Lama glama)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 1,2-dimethoxy-12-methyl[1,3]benzodioxolo[5,6-c]phenanthridin-12-ium (348 Da, Ligand)
- Cholinephosphotransferase 1 (44 kDa, Protein from Saccharomyces cerevisiae)
Cholinephosphotransferase in complex with diacylglycerol
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8ul9
Q-score: 0.503
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody24 (Complex from Lama glama)
- Cholinephosphotransferase 1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- Diacyl glycerol (625 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Saccharomyces cerevisiae cdp-choline phosphotransferase in complex with diacyl-glycerol (Complex from Saccharomyces cerevisiae)
CryoET of E. coli prepared with the Waffle Method
Kelley K, Raczkowski AM, Klykov O, Jaroenlak P, Bobe D, Kopylov M, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ
Nat Commun (2022) 13 pp. 1857-1857 [ DOI: doi:10.1038/s41467-022-29501-3 Pubmed: 35387991 ]
CryoET of E. coli prepared with the Waffle Method
Kelley K, Jaroenlak P, Raczkowski, AM, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ
bioRxiv (2020) [ DOI: 10.1101/2020.10.28.359372 ]
Image sets:
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol. Cell (2020) [ Pubmed: 32386575 DOI: 10.1016/j.molcel.2020.04.014 ]
Image sets:
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Wild-type
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol. Cell (2020) [ Pubmed: 32386575 DOI: 10.1016/j.molcel.2020.04.014 ]
Image sets:
Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6w98
Q-score: 0.535
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]
- Water (18 Da, Ligand)
- Acyl carrier protein (Cellular component from Escherichia coli (strain K12))
- Acyl carrier protein (8 kDa, Protein from Escherichia coli (strain K12))
- F5/8 type c domain-containing protein (149 kDa, Protein from Mycobacteroides abscessus subsp. abscessus)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- F5/8 type c domain-containing protein (Complex from Mycobacteroides abscessus subsp. abscessus)
- Calcium ion (40 Da, Ligand)
- Wild-type mycobacterial arabinofuranosyltransferase aftd complexed with acyl carrier protein (50 kDa, Cellular component)
- 4'-phosphopantetheine (358 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6wbx
Q-score: 0.518
Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F
Mol Cell (2020) 78 pp. 683- [ DOI: doi:10.1016/j.molcel.2020.04.014 Pubmed: 32386575 ]