Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 2)
Resolution: 2.43 Å
EM Method: Single-particle
Fitted PDBs: 8d2k
Q-score: 0.626
Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H
Nat Catal (2023) 6 pp. 969-977 [ DOI: doi:10.1038/s41929-023-01031-1 Pubmed: 38348449 ]
- Crispr-associated endonuclease, csn1 family/rna (Complex from Acidothermus cellulolyticus 11B)
- Cryoem structure of acecas9 (cleavage intermediate 2) (Complex)
- Dna non-target strand (5'-d(p*tp*ap*tp*ap*cp*ap*cp*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from Acidothermus cellulolyticus 11B)
- Water (18 Da, Ligand)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus 11B)
- Dna (Complex)
- Dna target strand (5'-d(p*cp*cp*ap*gp*gp*ap*tp*cp*tp*tp*gp*cp*cp*ap*tp*cp*cp*tp*ap*cp*cp*tp*cp*t)-3') (7 kDa, DNA from Acidothermus cellulolyticus 11B)
- Magnesium ion (24 Da, Ligand)
- Dna target strand (5'-d(*ap*gp*cp*tp*tp*gp*gp*tp*gp*tp*ap*tp*a)-3') (4 kDa, DNA from Acidothermus cellulolyticus 11B)
- Dna non-target strand (5'-d(p*ap*gp*a)-3') (910 Da, DNA from Acidothermus cellulolyticus 11B)
- Single guide rna (102-mer) (34 kDa, RNA from Acidothermus cellulolyticus)
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 1)
Resolution: 2.21 Å
EM Method: Single-particle
Fitted PDBs: 8d2l
Q-score: 0.661
Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H
Nat Catal (2023) 6 pp. 969-977 [ DOI: doi:10.1038/s41929-023-01031-1 Pubmed: 38348449 ]
- Crispr-associated endonuclease, csn1 family/rna (Complex from Acidothermus cellulolyticus 11B)
- Water (18 Da, Ligand)
- Single guide rna (106-mer) (34 kDa, RNA from Acidothermus cellulolyticus 11B)
- Dna non-target strand (5'-d(p*ap*gp*a)-3') (910 Da, DNA from synthetic construct)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus 11B)
- Dna (Complex)
- Dna target strand (5'-d(p*cp*cp*ap*gp*gp*ap*tp*cp*tp*tp*gp*cp*cp*ap*tp*cp*cp*tp*ap*cp*cp*tp*cp*t)-3') (7 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna target strand (5'-d(*ap*gp*cp*tp*tp*gp*gp*tp*gp*tp*ap*tp*a)-3') (4 kDa, DNA from synthetic construct)
- Dna non-target strand (5'-d(p*tp*ap*tp*ap*cp*ap*cp*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from synthetic construct)
- Cryoem structure of acecas9 (cleavage intermediate 1) (Complex)
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Pre-cleavage)
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8d2n
Q-score: 0.459
Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H
Nat Catal (2023) 6 pp. 969-977 [ DOI: doi:10.1038/s41929-023-01031-1 Pubmed: 38348449 ]
- Crispr-associated endonuclease, csn1 family/rna (Complex from Acidothermus cellulolyticus 11B)
- Cryoem structure of acecas9 (pre-cleavage) (Complex)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus 11B)
- Dna (Complex)
- Single guide rna (106-mer) (34 kDa, RNA from Acidothermus cellulolyticus 11B)
- Magnesium ion (24 Da, Ligand)
- Dna target strand (25-mer) (7 kDa, DNA from synthetic construct)
- Dna non-target strand (5'-d(p*tp*ap*cp*ap*cp*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from synthetic construct)
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 2)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8d2o
Q-score: 0.56
Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H
Nat Catal (2023) 6 pp. 969-977 [ DOI: doi:10.1038/s41929-023-01031-1 Pubmed: 38348449 ]
- Crispr-associated endonuclease, csn1 family/rna (Complex from Acidothermus cellulolyticus 11B)
- Water (18 Da, Ligand)
- Dna non-target strand (5'-d(p*ap*tp*ap*cp*ap*cp*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus 11B)
- Dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Cryoem structure of acecas9 (post-cleavage 2) (Complex)
- Dna target strand (5'-d(*ap*gp*cp*tp*tp*gp*gp*tp*gp*tp*ap*tp*a)-3') (4 kDa, DNA from synthetic construct)
- Dna target strand (5'-d(p*cp*cp*ap*gp*gp*ap*tp*cp*tp*tp*g)-3') (3 kDa, DNA from synthetic construct)
- Single guide rna (106-mer) (34 kDa, RNA from Acidothermus cellulolyticus 11B)
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Target bound)
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8d2p
Q-score: 0.572
Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H
Nat Catal (2023) 6 pp. 969-977 [ DOI: doi:10.1038/s41929-023-01031-1 Pubmed: 38348449 ]
- Crispr-associated endonuclease, csn1 family/rna (Complex from Acidothermus cellulolyticus 11B)
- Water (18 Da, Ligand)
- Single guide rna (102-mer) (34 kDa, RNA from Acidothermus cellulolyticus 11B)
- Cryoem structure of acecas9 (target bound) (Complex)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus 11B)
- Dna (Complex)
- Dna target strand (5'-d(p*cp*cp*ap*gp*gp*ap*tp*cp*tp*tp*gp*cp*cp*ap*tp*cp*cp*tp*ap*cp*cp*tp*cp*t)-3') (7 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 1)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8d2q
Q-score: 0.522
Das A, Rai J, Roth MO, Shu Y, Medina ML, Barakat MR, Li H
Nat Catal (2023) 6 pp. 969-977 [ DOI: doi:10.1038/s41929-023-01031-1 Pubmed: 38348449 ]
- Dna target strand (5'-d(p*cp*cp*ap*gp*gp*ap*tp*cp*tp*tp*g)-3') (3 kDa, DNA from synthetic construct)
- Single guide rna (86-mer) (34 kDa, RNA from Acidothermus cellulolyticus 11B)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus 11B)
- Water (18 Da, Ligand)
- Crispr-associated endonuclease, csn1 family/rna (Complex from Acidothermus cellulolyticus 11B)
- Dna target strand (5'-d(*ap*gp*cp*tp*tp*gp*gp*tp*gp*tp*ap*tp*a)-3') (4 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex)
- Cryoem structure of acecas9 (post-cleavage 1) (Complex)
- Dna non-target strand (5'-d(p*ap*tp*ap*cp*ap*cp*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from synthetic construct)