Structure of rabbit Slo1 in complex with gamma1/LRRC26
Resolution: 2.39 Å
EM Method: Single-particle
Fitted PDBs: 8s3e
Q-score: 0.521
Redhardt M, Raunser S, Raisch T
FEBS Lett (2024) 598 pp. 875-888 [ Pubmed: 38553946 DOI: doi:10.1002/1873-3468.14863 ]
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Water (18 Da, Ligand)
- Calcium-activated potassium channel subunit alpha-1 (126 kDa, Protein from Oryctolagus cuniculus)
- Leucine-rich repeat-containing protein 26 (35 kDa, Protein from Oryctolagus cuniculus)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Slo1-gamma1 complex (Complex from Oryctolagus cuniculus)
Human KMN network (outer kinetochore)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 8q5h
Q-score: 0.131
Polley S, Raisch T, Ghetti S, Korner M, Terbeck M, Grater F, Raunser S, Aponte-Santamaria C, Vetter IR, Musacchio A
Nat Struct Mol Biol (2024) 31 pp. 861-873 [ DOI: doi:10.1038/s41594-024-01230-9 Pubmed: 38459128 ]
- Kinetochore scaffold 1 (38 kDa, Protein from Homo sapiens)
- Protein mis12 homolog (24 kDa, Protein from Homo sapiens)
- Kinetochore protein spc25 (16 kDa, Protein from Homo sapiens)
- Kinetochore-associated protein nsl1 homolog (32 kDa, Protein from Homo sapiens)
- Kinetochore-associated protein dsn1 homolog (40 kDa, Protein from Homo sapiens)
- Kinetochore protein spc24 (10 kDa, Protein from Homo sapiens)
- Polyamine-modulated factor 1 (23 kDa, Protein from Homo sapiens)
- Kmn network (Complex from Homo sapiens)
Structure of the human inner kinetochore CCAN complex
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7qoo
Q-score: 0.202
Pesenti ME, Raisch T, Conti D, Walstein K, Hoffmann I, Vogt D, Prumbaum D, Vetter IR, Raunser S, Musacchio A
Mol Cell (2022) 82 pp. 2113- [ DOI: doi:10.1016/j.molcel.2022.04.027 Pubmed: 35525244 ]
- Centromere protein k (31 kDa, Protein from Homo sapiens)
- Centromere protein t (60 kDa, Protein from Homo sapiens)
- Centromere protein w (10 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
- Human inner kinetochore ccan complex (451 kDa, Complex from Homo sapiens)
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Unknown protein (1 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
- Centromere protein c (61 kDa, Protein from Homo sapiens)
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein u (47 kDa, Protein from Homo sapiens)
STRUCTURE OF THE HUMAN INNER KINETOCHORE CCAN COMPLEX AT 10A RESOLUTION
Pesenti ME, Raisch T, Conti D, Walstein K, Hoffmann I, Vogt D, Prumbaum D, Vetter IR, Raunser S, Musacchio A
Mol Cell (2022) 82 pp. 2113-2131.e8 [ DOI: doi:10.1016/j.molcel.2022.04.027 Pubmed: 35525244 ]
- Human inner kinetochore ccan complex (451 kDa, Complex from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zgu
Q-score: 0.289
Raisch T, Machtens DA, Bresch IB, Eberhage J, Prumbaum D, Reubold TF, Raunser S, Eschenburg S
To Be Published
- Nacht, lrr and pyd domains-containing protein 3 (105 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nlrp3 hexamer (Complex from Homo sapiens)
Human RZZ kinetochore corona complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7qpg
Q-score: 0.202
Raisch T, Ciossani G, d'Amico E, Cmentowski V, Carmignani S, Maffini S, Merino F, Wohlgemuth S, Vetter IR, Raunser S, Musacchio A
EMBO J (2022) 41 pp. e110411-e110411 [ DOI: doi:10.15252/embj.2021110411 Pubmed: 35373361 ]
- Centromere/kinetochore protein zw10 homolog (88 kDa, Protein from Homo sapiens)
- Protein zwilch homolog (67 kDa, Protein from Homo sapiens)
- Kinetochore-associated protein 1 (279 kDa, Protein from Homo sapiens)
- Rzz complex (Complex from Homo sapiens)
Ca2+ bound Drosophila Slo channel
Resolution: 2.38 Å
EM Method: Single-particle
Fitted PDBs: 7pxe
Q-score: 0.604
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ DOI: doi:10.1038/s41467-021-27435-w Pubmed: 34887422 ]
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Slo tetramer (Complex from Drosophila melanogaster)
Ca2+ free Drosophila Slo channel
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7pxf
Q-score: 0.485
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ DOI: doi:10.1038/s41467-021-27435-w Pubmed: 34887422 ]
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Slo tetramer (Complex from Drosophila melanogaster)
- Potassium ion (39 Da, Ligand)
Verruculogen-bound Drosophila Slo channel
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 7pxg
Q-score: 0.552
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ DOI: doi:10.1038/s41467-021-27435-w Pubmed: 34887422 ]
- Potassium ion (39 Da, Ligand)
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- Slo tetramer (Complex from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Verruculogen (511 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Emodepside-bound Drosophila Slo channel
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 7pxh
Q-score: 0.569
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ DOI: doi:10.1038/s41467-021-27435-w Pubmed: 34887422 ]
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- (3~{s},6~{r},9~{s},12~{r},15~{s},18~{r},21~{s},24~{r})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22-tetrazacyclotetracosane-2,5,8,11,14,17,20,23-octone (1 kDa, Ligand)
- Calcium ion (40 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Slo tetramer (Complex from Drosophila melanogaster)