Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Phalloidin. (Complex from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Cryo-EM reconstruction of the formin Cdc12 bound to the barbed end of F-actin (without phalloidin)
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Actin filament (Complex)
- S. pombe cdc12 (Protein from Schizosaccharomyces pombe)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of f-actin. (Complex from Homo sapiens)
- Human cytoplasmic beta-actin (Protein from Homo sapiens)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Inf2 dimer (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, K1179W mutant
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8cpz
Q-score: 0.526
Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S
Sci Adv (2025) 11 pp. eadr2019-eadr2019 [ Pubmed: 39752508 DOI: doi:10.1126/sciadv.adr2019 ]
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, K567W K2008W mutant
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8cq0
Q-score: 0.466
Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S
Sci Adv (2025) 11 pp. eadr2019-eadr2019 [ Pubmed: 39752508 DOI: doi:10.1126/sciadv.adr2019 ]
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, C16S, C20S, C870S, T1279C mutant
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8cq2
Q-score: 0.448
Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S
Sci Adv (2025) 11 pp. eadr2019-eadr2019 [ Pubmed: 39752508 DOI: doi:10.1126/sciadv.adr2019 ]
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Structure of Yersinia entomophaga Tc toxin from FIB-milled spheroplasts