Cryo-EM structure of the phalloidin-bound pointed end of the actin filament.
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 9fjm
Q-score: 0.476
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ DOI: doi:10.1038/s41467-024-52251-3 Pubmed: 39261469 ]
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Phosphate ion (94 Da, Ligand)
- Actin filament pointed end (Complex from Homo sapiens)
- Phalloidin (Complex from Amanita phalloides)
- Actin filament pointed end bound by phalloidin (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 1)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 9fju
Q-score: 0.399
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ DOI: doi:10.1038/s41467-024-52251-3 Pubmed: 39261469 ]
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Bos taurus)
- Deoxyribonuclease-1 (31 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament pointed end (Complex from Bos taurus)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Two dnase i molecules, each bound to the ultimate and penultimate actin subunits of the f-actin pointed end. (Complex from Bos taurus)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- F-actin pointed end bound by dnase i and phalloidin. (Complex)
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 2).
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 9fjy
Q-score: 0.412
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ DOI: doi:10.1038/s41467-024-52251-3 Pubmed: 39261469 ]
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Bos taurus)
- Deoxyribonuclease-1 (31 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament pointed end (Complex from Bos taurus)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Two dnase i molecules, each bound to the ultimate and penultimate actin subunits of the f-actin pointed end. (Complex from Bos taurus)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- F-actin pointed end bound by dnase i and phalloidin. (Complex)
Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Phalloidin. (Complex from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Phosphate ion (94 Da, Ligand)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Phalloidin (Complex from Amanita phalloides)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
Cryo-EM structure of the undecorated barbed end of filamentous beta/gamma actin
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 8oi6
Q-score: 0.464
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ DOI: doi:10.1038/s41594-023-01101-9 Pubmed: 37749275 ]
- Phalloidin (Complex from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Actin-phalloidin complex (Complex)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bos taurus)
- Cytosolic beta-gamma actin (Complex from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er)
Resolution: 3.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 7plt
Q-score: 0.465
Pospich S, Sweeney HL, Houdusse A, Raunser S
eLife (2021) 10 [ Pubmed: 34812732 DOI: doi:10.7554/eLife.73724 ]
- Magnesium ion (24 Da, Ligand)
- Myosin light chain 6b (17 kDa, Protein from Homo sapiens)
- Actomyosin-v complex in the rigor state (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unconventional myosin-va (91 kDa, Protein from Gallus gallus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er)
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 7plu
Q-score: 0.455
Pospich S, Sweeney HL, Houdusse A, Raunser S
eLife (2021) 10 [ Pubmed: 34812732 DOI: doi:10.7554/eLife.73724 ]
- Magnesium ion (24 Da, Ligand)
- Myosin light chain 6b (17 kDa, Protein from Homo sapiens)
- Actomyosin-v complex in the rigor state (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unconventional myosin-va (91 kDa, Protein from Gallus gallus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4)
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7plx
Q-score: 0.405
Pospich S, Sweeney HL, Houdusse A, Raunser S
eLife (2021) 10 [ Pubmed: 34812732 DOI: doi:10.7554/eLife.73724 ]
- Magnesium ion (24 Da, Ligand)
- Myosin light chain 6b (17 kDa, Protein from Homo sapiens)
- Actomyosin-v complex in the rigor state (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unconventional myosin-va (91 kDa, Protein from Gallus gallus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er)
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 7pm5
Q-score: 0.449
Pospich S, Sweeney HL, Houdusse A, Raunser S
eLife (2021) 10 [ DOI: doi:10.7554/eLife.73724 Pubmed: 34812732 ]
- Actomyosin-v complex in the strong-adp state (Complex)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Unconventional myosin-va (91 kDa, Protein from Gallus gallus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Myosin light chain 6b (17 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)