Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Phalloidin. (Complex from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Phosphate ion (94 Da, Ligand)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Phalloidin (Complex from Amanita phalloides)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Phosphate ion (94 Da, Ligand)
Structure of the F-actin barbed end bound by formin mDia1
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8ru2
Q-score: 0.362
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,protein diaphanous homolog 1 (86 kDa, Protein from Mus musculus)
- Actin filament (Complex from Homo sapiens)
- Mdia1-bound f-actin barbed end. (Complex)
- Mouse mdia1 (fh1fh2c domain) (Complex from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Inf2 dimer (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
Structure of P. luminescens TccC3-F-actin complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7z7h
Q-score: 0.366
Belyy A, Lindemann F, Roderer D, Funk J, Bardiaux B, Protze J, Bieling P, Oschkinat H, Raunser S
Nat Commun (2022) 13 pp. 4202-4202 [ Pubmed: 35858890 DOI: doi:10.1038/s41467-022-31836-w ]
- Magnesium ion (24 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,tccc3 (63 kDa, Protein from Photorhabdus luminescens)
- Nicotinamide (122 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Structure of p. luminescence tccc3-f-actin complex (Complex from Photorhabdus luminescens)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Adenosine-5-diphosphoribose (559 Da, Ligand)
Structure of ADP-ribosylated F-actin
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7z7i
Q-score: 0.444
Belyy A, Lindemann F, Roderer D, Funk J, Bardiaux B, Protze J, Bieling P, Oschkinat H, Raunser S
Nat Commun (2022) 13 pp. 4202-4202 [ Pubmed: 35858890 DOI: doi:10.1038/s41467-022-31836-w ]
- Structure of adp-ribosylated f-actin (Complex from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Adenosine-5-diphosphoribose (559 Da, Ligand)
Structure of capping protein bound to the barbed end of a cytoplasmic actin filament
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7pdz
Q-score: 0.333
Funk J, Merino F, Schaks M, Rottner K, Raunser S, Bieling P
Nat Commun (2021) 12 pp. 5329-5329 [ DOI: doi:10.1038/s41467-021-25682-5 Pubmed: 34504078 ]
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bovine)
- Complex between capping protein and the barbed end of cytoplasmic actin filaments (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (808 Da, Protein from synthetic construct)
- Isoform 2 of f-actin-capping protein subunit beta (30 kDa, Protein from Mus musculus)
- Phosphate ion (94 Da, Ligand)
- F-actin-capping protein subunit alpha-1 (32 kDa, Protein from Mus musculus)
Cryo-EM structure of F-actin stabilized by cis-optoJASP-8
Resolution: 2.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 7ahn
Q-score: 0.553
Pospich S, Kullmer F, Nasufovic V, Funk J, Belyy A, Bieling P, Arndt HD, Raunser S
Angew Chem Int Ed Engl (2021) 60 pp. 8678-8682 [ DOI: doi:10.1002/anie.202013193 Pubmed: 33449370 ]
- Magnesium ion (24 Da, Ligand)
- Filamentous alpha actin stabilized by cis-optojasp-8 in complex with adp-pi (Complex from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- ~{n}-[4-[(4~{r},7~{r},10~{s},13~{s},15~{e},19~{s})-4-(4-hydroxyphenyl)-7-(1~{h}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{n}'-[5-methoxy-2-[(~{z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide (1 kDa, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Phosphate ion (94 Da, Ligand)
Cryo-EM structure of F-actin stabilized by trans-optoJASP-8
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7ahq
Q-score: 0.46
Pospich S, Kullmer F, Nasufovic V, Funk J, Belyy A, Bieling P, Arndt HD, Raunser S
Angew Chem Int Ed Engl (2021) 60 pp. 8678-8682 [ DOI: doi:10.1002/anie.202013193 Pubmed: 33449370 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Filamentous alpha actin stabilized by trans-optojasp-8 in complex with adp-pi (Complex from Oryctolagus cuniculus)
- ~{n}-[4-[(4~{r},7~{r},10~{s},13~{s},15~{e},19~{s})-4-(4-hydroxyphenyl)-7-(1~{h}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-2,6,9,12-tetrakis(oxidanylidene)-1-oxa-5,8,11-triazacyclononadec-15-en-10-yl]butyl]-~{n}'-[5-methoxy-2-[(~{z})-(3,4,5-trimethoxyphenyl)diazenyl]phenyl]butanediamide (1 kDa, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Phosphate ion (94 Da, Ligand)