Structure of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 7zf1
Q-score: 0.31
Lemonidis K, Rennie ML, Arkinson C, Chaugule VK, Clarke M, Streetley J, Walden H
EMBO J (2023) 42 pp. e111898-e111898 [ Pubmed: 36385258 DOI: doi:10.15252/embj.2022111898 ]
- Ubiquitin-60s ribosomal protein l40 (8 kDa, Protein from Homo sapiens)
- Fanconi anemia group d2 protein (166 kDa, Protein from Homo sapiens)
- Complex of ubiquitinated fanci with fancd2 and double-stranded dna. (Complex from Homo sapiens)
- Dna (61-mer) (8 kDa, DNA from synthetic construct)
- Fanconi anemia group i protein (152 kDa, Protein from Homo sapiens)
- Dna (61-mer) (8 kDa, DNA from synthetic construct)
Rennie ML, Arkinson C, Chaugule VK, Walden H
Sci Adv (2022) 8 pp. eabq6353-eabq6353 [ DOI: doi:10.1126/sciadv.abq6353 Pubmed: 36170365 ]
- Fanconi anemia group i protein (Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 1 (Protein from Homo sapiens)
- Polyubiquitin-c (Protein from Homo sapiens)
- Dna (61-mer) (DNA from synthetic construct)
- Fanconi anemia group d2 protein (Protein from Homo sapiens)
- Wd repeat-containing protein 48 (Protein from Homo sapiens)
- Fanconi anemia group i protein, fanconi anemia group d2 protein with ubiquitin conjugated to k561, ubiquitin carboxyl-terminal hydrolase 1, wd repeat-containing protein 48 (Complex from Homo sapiens)
- Usp1(c90s)-uaf1 bound to fanci and mono-ubiquitinated fancd2 with dsdna and ml323 (Complex)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8a9j
Q-score: 0.553
Rennie ML, Arkinson C, Chaugule VK, Walden H
Sci Adv (2022) 8 pp. eabq6353-eabq6353 [ DOI: doi:10.1126/sciadv.abq6353 Pubmed: 36170365 ]
- Fanconi anemia group d2 protein (164 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 48 (78 kDa, Protein from Homo sapiens)
- Fanconi anemia group i protein (150 kDa, Protein from Homo sapiens)
- Dna (61-mer) (5 kDa, DNA from synthetic construct)
- Usp1(c90s)-uaf1 bound to fanci and mono-ubiquitinated fancd2 with dsdna (Complex)
- Zinc ion (65 Da, Ligand)
- Ubiquitin carboxyl-terminal hydrolase 1 (88 kDa, Protein from Homo sapiens)
- Polyubiquitin-c (8 kDa, Protein from Homo sapiens)
- Fanconi anemia group i protein, fanconi anemia group d2 protein with ubiquitin conjugated to k561, ubiquitin carboxyl-terminal hydrolase 1, wd repeat-containing protein 48 (Complex from Homo sapiens)
Cryo-EM structure of USP1-UAF1 bound to mono-ubiquitinated FANCD2, and FANCI
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7ay1
Q-score: 0.432
Rennie ML, Arkinson C, Chaugule VK, Toth R, Walden H
Nat Struct Mol Biol (2021) 28 pp. 356-364 [ Pubmed: 33795880 DOI: doi:10.1038/s41594-021-00576-8 ]
- Fanconi anemia group d2 protein (164 kDa, Protein from Homo sapiens)
- Enzyme substrate complex of usp1-uaf1 and fanci-fancd2ub-dsdna (490 kDa, Complex)
- Fanconi anemia group i protein (150 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (8 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 48 (78 kDa, Protein from Homo sapiens)
- Dna (61-mer) (5 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Ubiquitin carboxyl-terminal hydrolase 1 (88 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Enzyme substrate complex of usp1-uaf1 and fanci-fancd2ub (Complex from Homo sapiens)
Mono-ubiquitinated FANCD2 in complex with mono-ubiquitinated FANCI and dsDNA
Rennie ML, Lemonidis K, Arkinson C, Chaugule VK, Clarke M, Streetley J, Spagnolo L, Walden H
EMBO Rep (2020) 21 pp. e50133-e50133 [ DOI: doi:10.15252/embr.202050133 Pubmed: 32510829 ]
- Ternary complex of mono-ubiquitinated fancd2, mono-ubiquitinated fanci, and 32 base pair dsdna (350 kDa, Complex)
- Double stranded dna, 32 base pairs (DNA from synthetic construct)
- 32 base pair dsdna (Complex from synthetic construct)
- Fancd2 mono-ubiquitinated at k561 (Protein from Homo sapiens)
- Mono-ubiquitinated fancd2, mono-ubiquitinated fanci (Complex from Homo sapiens)
- Fanci mono-ubiquitinated at k523 (Protein from Homo sapiens)