CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6oax
Q-score: 0.5
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ DOI: doi:10.1038/s41467-019-10150-y Pubmed: 31160557 ]
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, post-state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6oay
Q-score: 0.437
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6og1
Q-score: 0.304
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Hyperactive disaggregase clpb k476c, bound to casein (Complex from Escherichia coli K-12)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, post-state
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6og2
Q-score: 0.158
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6og3
Q-score: 0.306
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Hyperactive disaggregase clpb (96 kDa, Protein from Escherichia coli K-12)
- Hyperactive clpb mutant k476c_ntd trimer (Complex from Escherichia coli K-12)
- Alpha s1-casein (783 Da, Protein from Bos taurus)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)