V-K CAST Transpososome from Scytonema hofmanni, minor configuration
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ea4
Q-score: 0.302
Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH
Nature (2023) 613 pp. 775-782 [ Pubmed: 36442503 DOI: doi:10.1038/s41586-022-05573-5 ]
- Non-target_r (21 kDa, DNA from synthetic construct)
- Cas12k (73 kDa, Protein from Scytonema hofmannii)
- Sg_rna (85 kDa, RNA from synthetic construct)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Tniq (19 kDa, Protein from Scytonema hofmannii)
- Re_r1 (15 kDa, DNA from synthetic construct)
- Re_f (23 kDa, DNA from synthetic construct)
- Sh_cast transpososome (Complex from [Scytonema hofmanni] UTEX 2349)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Le_r (15 kDa, DNA from synthetic construct)
- Target-le (43 kDa, DNA from synthetic construct)
- Tnsb (66 kDa, Protein from Scytonema hofmannii)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Magnesium ion (24 Da, Ligand)
- Re_r2 (6 kDa, DNA from synthetic construct)
ClpA/ClpP Disengaged State bound to RepA-GFP
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6uqe
Q-score: 0.493
Lopez KE, Rizo AN, Tse E, Lin J, Scull NW, Thwin AC, Lucius AL, Shorter J, Southworth DR
Nat Struct Mol Biol (2020) 27 pp. 406-416 [ DOI: doi:10.1038/s41594-020-0409-5 Pubmed: 32313240 ]
- Atp-dependent clp protease atp-binding subunit clpa (64 kDa, Protein from Escherichia coli K-12)
- Repa-gfp (869 Da, Protein from unidentified)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Repa-gfp (954 Da, Protein from unidentified)
- Atp-dependent clp protease proteolytic subunit (21 kDa, Protein from Escherichia coli K-12)
- Clpap (840 kDa, Complex from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6oax
Q-score: 0.5
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ DOI: doi:10.1038/s41467-019-10150-y Pubmed: 31160557 ]
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, post-state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6oay
Q-score: 0.437
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6og1
Q-score: 0.304
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Hyperactive disaggregase clpb k476c, bound to casein (Complex from Escherichia coli K-12)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, post-state
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6og2
Q-score: 0.158
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6og3
Q-score: 0.306
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Hyperactive disaggregase clpb (96 kDa, Protein from Escherichia coli K-12)
- Hyperactive clpb mutant k476c_ntd trimer (Complex from Escherichia coli K-12)
- Alpha s1-casein (783 Da, Protein from Bos taurus)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)