Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t4q
Q-score: 0.54
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab 2c12 light chain (25 kDa, Protein from Homo sapiens)
- Fab 2c12 heavy chain (27 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Fab 7i13 light chain (25 kDa, Protein from Homo sapiens)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Pentameric complex of hcmv proteins gh, gl, ul128, ul130, ul131a bound to fabs of human neutralizing antibodies 2c12, 7i13 and 13h11 (320 kDa, Complex)
- Fab 7i13 heavy chain (27 kDa, Protein from Homo sapiens)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7t4r
Q-score: 0.429
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Thrombomodulin (55 kDa, Protein from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- Complex of 2x hcmv pentamer gh, gl, ul128, ul130, ul131a bound to 1x thbd and 2x fabs of human neutralizing antibodies msl-109 and 13h11 (590 kDa, Complex)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7t4s
Q-score: 0.57
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Fab 8i21 light chain (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Fab 8i21 heavy chain (26 kDa, Protein from Homo sapiens)
- Neuropilin-2 (98 kDa, Protein from Homo sapiens)
- Pentameric complex of hcmv proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and fabs of human neutralizing antibodies 8i21 and 13h11 (360 kDa, Complex)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Hcmv envelope glycoprotein h (Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul128 (Protein from Human betaherpesvirus 5)
- Hcmv envelope glycoprotein ul130 (Protein from Human betaherpesvirus 5)
- Hcmv envelope glycoprotein l (Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (Protein from Homo sapiens)
- Complex of 2x hcmv pentamer proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and 2x fabs of human neutralizing antibodies 8i21 and 13h11 (720 kDa, Complex)
- Fab 8i21 light chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul131a (Protein from Human betaherpesvirus 5)
- Fab 8i21 heavy chain (Protein from Homo sapiens)
- Neuropilin-2 (Protein from Homo sapiens)
Structure of human TRPA1 in complex with antagonist compound 21
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7jup
Q-score: 0.522
Terrett JA, Chen H, Shore DG, Villemure E, Larouche-Gauthier R, Dery M, Beaumier F, Constantineau-Forget L, Grand-Maitre C, Lepissier L, Ciblat S, Sturino C, Chen Y, Hu B, Lu A, Wang Y, Cridland AP, Ward SI, Hackos DH, Reese RM, Shields SD, Chen J, Balestrini A, Riol-Blanco L, Lee WP, Liu J, Suto E, Wu X, Zhang J, Ly JQ, La H, Johnson K, Baumgardner M, Chou KJ, Rohou A, Rouge L, Safina BS, Magnuson S, Volgraf M
J Med Chem (2021) 64 pp. 3843-3869 [ Pubmed: 33749283 DOI: doi:10.1021/acs.jmedchem.0c02023 ]
CryoEM structure of the HCMV Trimer gHgLgO in complex with neutralizing fabs 13H11 and MSL-109
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lbe
Q-score: 0.542
Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell (2021) 184 pp. 1232- [ DOI: doi:10.1016/j.cell.2021.01.036 Pubmed: 33626330 ]
- Envelope glycoprotein o (58 kDa, Protein from Human cytomegalovirus)
- Hcmv trimer ghglgo bound to neutralizing fabs 13h11 and msl-109 (275 kDa, Complex)
- Neutralizing fabs 13h11 and msl-109 (Complex from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hcmv trimer ghglgo (Complex from Human cytomegalovirus)
- Envelope glycoprotein l (30 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Envelope glycoprotein h (87 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Alpha-d-mannopyranose (180 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7lbf
Q-score: 0.548
Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell (2021) 184 pp. 1232- [ DOI: doi:10.1016/j.cell.2021.01.036 Pubmed: 33626330 ]
- Envelope glycoprotein o (58 kDa, Protein from Human cytomegalovirus)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hcmv trimer ghglgo (Complex from Human cytomegalovirus)
- Isoform 3 of platelet-derived growth factor receptor alpha (59 kDa, Protein from Homo sapiens)
- Envelope glycoprotein l (30 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Envelope glycoprotein h (87 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Pdgfra and neutralizing fabs 13h11 and msl-109 (Complex from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo bound to human receptor pdgfra and neutralizing fabs 13h11 and msl-109 (335 kDa, Complex)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7lbg
Q-score: 0.604
Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell (2021) 184 pp. 1232- [ DOI: doi:10.1016/j.cell.2021.01.036 Pubmed: 33626330 ]
- Envelope glycoprotein o (58 kDa, Protein from Human cytomegalovirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neutralizing fabs 13h11 and msl-109 (Complex from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo (Complex from Human cytomegalovirus, Human cytomegalovirus)
- Envelope glycoprotein l (30 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Transforming growth factor beta receptor type 3 (87 kDa, Protein from Homo sapiens)
- Envelope glycoprotein h (87 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo bound to human receptor tgfbr3 and neutralizing fabs 13h11 and msl-109 (360 kDa, Complex)
Structure of human TRPA1 in complex with inhibitor GDC-0334
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6wj5
Q-score: 0.471
Balestrini A, Joseph V, Dourado M, Reese RM, Shields SD, Rouge L, Bravo DD, Chernov-Rogan T, Austin CD, Chen H, Wang L, Villemure E, Shore DGM, Verma VA, Hu B, Chen Y, Leong L, Bjornson C, Hotzel K, Gogineni A, Lee WP, Suto E, Wu X, Liu J, Zhang J, Gandham V, Wang J, Payandeh J, Ciferri C, Estevez A, Arthur CP, Kortmann J, Wong RL, Heredia JE, Doerr J, Jung M, Vander Heiden JA, Roose-Girma M, Tam L, Barck KH, Carano RAD, Ding HT, Brillantes B, Tam C, Yang X, Gao SS, Ly JQ, Liu L, Chen L, Liederer BM, Lin JH, Magnuson S, Chen J, Hackos DH, Elstrott J, Rohou A, Safina BS, Volgraf M, Bauer RN, Riol-Blanco L
J Exp Med (2021) 218 [ DOI: doi:10.1084/jem.20201637 Pubmed: 33620419 ]
- Transient receptor potential cation channel subfamily a member 1 (72 kDa, Protein from Homo sapiens)
- Trpa1 bound by inhibitor gdc-0334 (Complex from Homo sapiens)
- (4r,5s)-4-fluoro-1-[(4-fluorophenyl)sulfonyl]-5-methyl-n-({5-(trifluoromethyl)-2-[2-(trifluoromethyl)pyrimidin-5-yl]pyridin-4-yl}methyl)-l-prolinamide (609 Da, Ligand)
Structure of human TRPA1 in complex with agonist GNE551
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6x2j
Q-score: 0.509
Liu C, Reese R, Vu S, Rouge L, Shields SD, Kakiuchi-Kiyota S, Chen H, Johnson K, Shi YP, Chernov-Rogan T, Greiner DMZ, Kohli PB, Hackos D, Brillantes B, Tam C, Li T, Wang J, Safina B, Magnuson S, Volgraf M, Payandeh J, Zheng J, Rohou A, Chen J
Neuron (2021) 109 pp. 273-284.e4 [ DOI: doi:10.1016/j.neuron.2020.10.014 Pubmed: 33152265 ]