Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7t4q
Q-score: 0.54
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Fab 7i13 heavy chain (27 kDa, Protein from Homo sapiens)
- Fab 7i13 light chain (25 kDa, Protein from Homo sapiens)
- Pentameric complex of hcmv proteins gh, gl, ul128, ul130, ul131a bound to fabs of human neutralizing antibodies 2c12, 7i13 and 13h11 (320 kDa, Complex)
- Fab 2c12 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab 2c12 light chain (25 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7t4r
Q-score: 0.429
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Complex of 2x hcmv pentamer gh, gl, ul128, ul130, ul131a bound to 1x thbd and 2x fabs of human neutralizing antibodies msl-109 and 13h11 (590 kDa, Complex)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Thrombomodulin (55 kDa, Protein from Homo sapiens)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7t4s
Q-score: 0.57
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Envelope glycoprotein ul130 (28 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein l (30 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (87 kDa, Protein from Human betaherpesvirus 5)
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Envelope protein ul131a (15 kDa, Protein from Human betaherpesvirus 5)
- Fab 8i21 heavy chain (26 kDa, Protein from Homo sapiens)
- Neuropilin-2 (98 kDa, Protein from Homo sapiens)
- Fab 8i21 light chain (25 kDa, Protein from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Envelope protein ul128 (19 kDa, Protein from Human betaherpesvirus 5)
- Pentameric complex of hcmv proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and fabs of human neutralizing antibodies 8i21 and 13h11 (360 kDa, Complex)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Kschonsak M, Johnson MC, Schelling R, Green EM, Rouge L, Ho H, Patel N, Kilic C, Kraft E, Arthur CP, Rohou AL, Comps-Agrar L, Martinez-Martin N, Perez L, Payandeh J, Ciferri C
Sci Adv (2022) 8 pp. eabm2536-eabm2536 [ Pubmed: 35275719 DOI: doi:10.1126/sciadv.abm2536 ]
- Hcmv envelope glycoprotein ul131a (Protein from Human betaherpesvirus 5)
- Hcmv envelope glycoprotein l (Protein from Human betaherpesvirus 5)
- Neuropilin-2 (Protein from Homo sapiens)
- Fab 8i21 heavy chain (Protein from Homo sapiens)
- Fab 13h11 light chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul128 (Protein from Human betaherpesvirus 5)
- Fab 8i21 light chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein h (Protein from Human betaherpesvirus 5)
- Complex of 2x hcmv pentamer proteins gh, gl, ul128, ul130, ul131a bound to neuropilin 2 and 2x fabs of human neutralizing antibodies 8i21 and 13h11 (720 kDa, Complex)
- Fab 13h11 heavy chain (Protein from Homo sapiens)
- Hcmv envelope glycoprotein ul130 (Protein from Human betaherpesvirus 5)
CryoEM structure of the HCMV Trimer gHgLgO in complex with neutralizing fabs 13H11 and MSL-109
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lbe
Q-score: 0.542
Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell (2021) 184 pp. 1232- [ DOI: doi:10.1016/j.cell.2021.01.036 Pubmed: 33626330 ]
- Envelope glycoprotein o (58 kDa, Protein from Human cytomegalovirus)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Envelope glycoprotein h (87 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Envelope glycoprotein l (30 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Alpha-d-mannopyranose (180 Da, Ligand)
- Neutralizing fabs 13h11 and msl-109 (Complex from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo (Complex from Human cytomegalovirus)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo bound to neutralizing fabs 13h11 and msl-109 (275 kDa, Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7lbf
Q-score: 0.548
Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell (2021) 184 pp. 1232- [ DOI: doi:10.1016/j.cell.2021.01.036 Pubmed: 33626330 ]
- Envelope glycoprotein o (58 kDa, Protein from Human cytomegalovirus)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Envelope glycoprotein h (87 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Envelope glycoprotein l (30 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Pdgfra and neutralizing fabs 13h11 and msl-109 (Complex from Homo sapiens)
- Hcmv trimer ghglgo (Complex from Human cytomegalovirus)
- Hcmv trimer ghglgo bound to human receptor pdgfra and neutralizing fabs 13h11 and msl-109 (335 kDa, Complex)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Isoform 3 of platelet-derived growth factor receptor alpha (59 kDa, Protein from Homo sapiens)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7lbg
Q-score: 0.604
Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C
Cell (2021) 184 pp. 1232- [ DOI: doi:10.1016/j.cell.2021.01.036 Pubmed: 33626330 ]
- Envelope glycoprotein o (58 kDa, Protein from Human cytomegalovirus)
- Fab msl-109 light chain (28 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo bound to human receptor tgfbr3 and neutralizing fabs 13h11 and msl-109 (360 kDa, Complex)
- Envelope glycoprotein h (87 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Envelope glycoprotein l (30 kDa, Protein from Human cytomegalovirus (strain Merlin))
- Fab 13h11 heavy chain (26 kDa, Protein from Homo sapiens)
- Neutralizing fabs 13h11 and msl-109 (Complex from Homo sapiens)
- Fab 13h11 light chain (25 kDa, Protein from Homo sapiens)
- Transforming growth factor beta receptor type 3 (87 kDa, Protein from Homo sapiens)
- Hcmv trimer ghglgo (Complex from Human cytomegalovirus, Human cytomegalovirus)
- Fab msl-109 heavy chain (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)