Cryo-EM structure of zebrafish TRPM5 in the presence of 1 mM EDTA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mbp
Q-score: 0.534
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 5 mM calcium
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7mbq
Q-score: 0.604
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Water (18 Da, Ligand)
Cryo-EM structure of zebrafish TRPM5 in the presence of 6 uM calcium (apo state)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 6 uM calcium (open state)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Water (18 Da, Ligand)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Trpm5 channel e337a mutant (Complex from Danio rerio)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Trpm5 channel e337a mutant (Complex from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 5 mM calcium and 0.5 mM NDNA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mbv
Q-score: 0.546
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- N'-[(e)-(3,4-dimethoxyphenyl)methylidene]-2-(naphthalen-1-yl)acetohydrazide (348 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
Cryo-EM structure of wild type human Pannexin 1 channel
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 6wbf
Q-score: 0.492
Nature (2020) 584 pp. 646-651 [ Pubmed: 32494015 DOI: doi:10.1038/s41586-020-2357-y ]
- Wild type human pannexin 1 channel (Complex from Homo sapiens)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Pannexin-1 (42 kDa, Protein from Homo sapiens)
- Diacyl glycerol (625 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of human Pannexin 1 channel with its C-terminal tail cleaved by caspase-7
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 6wbg
Q-score: 0.514
Nature (2020) 584 pp. 646-651 [ Pubmed: 32494015 DOI: doi:10.1038/s41586-020-2357-y ]
- Wild type human pannexin 1 channel (Complex from Homo sapiens)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Pannexin-1 (42 kDa, Protein from Homo sapiens)
- Diacyl glycerol (625 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of human Pannexin 1 channel N255A mutant
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 6wbm
Q-score: 0.47
Nature (2020) 584 pp. 646-651 [ Pubmed: 32494015 DOI: doi:10.1038/s41586-020-2357-y ]
- Wild type human pannexin 1 channel (Complex from Homo sapiens)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Pannexin-1 (42 kDa, Protein from Homo sapiens)
- Diacyl glycerol (625 Da, Ligand)
- Cholesterol (386 Da, Ligand)