Human PAC in nanodisc at pH 4.0 with PI(4,5)P2 diC8
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8eq4
Q-score: 0.516
Mihaljevic L, Ruan Z, Osei-Owusu J, Lu W, Qiu Z
eLife (2023) 12 [ Pubmed: 36633397 DOI: doi:10.7554/eLife.83935 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Proton activated chloride channel at ph 4.0 with 0.5mm pip2 (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Proton-activated chloride channel (40 kDa, Protein from Homo sapiens)
Human PAC in nanodisc at pH 4.0 with PI(4,5)P2 diC8
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8fbl
Q-score: 0.538
Mihaljevic L, Ruan Z, Osei-Owusu J, Lu W, Qiu Z
eLife (2023) 12 [ Pubmed: 36633397 DOI: doi:10.7554/eLife.83935 ]
- Proton-activated chloride channel (32 kDa, Protein from Homo sapiens)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Proton activated chloride channel at ph 4.0 with 0.5mm pip2 (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of zebrafish TRPM5 in the presence of 1 mM EDTA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mbp
Q-score: 0.534
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 5 mM calcium
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 7mbq
Q-score: 0.604
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Water (18 Da, Ligand)
Cryo-EM structure of zebrafish TRPM5 in the presence of 6 uM calcium (apo state)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 6 uM calcium (open state)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- Water (18 Da, Ligand)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Trpm5 channel e337a mutant (Complex from Danio rerio)
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Trpm5 channel e337a mutant (Complex from Danio rerio)
Cryo-EM structure of zebrafish TRPM5 in the presence of 5 mM calcium and 0.5 mM NDNA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mbv
Q-score: 0.546
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lu W
Nat Struct Mol Biol (2021) 28 pp. 604-613 [ Pubmed: 34168372 DOI: doi:10.1038/s41594-021-00607-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- (2r)-2-(hydroxymethyl)-4-{[(25r)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (841 Da, Ligand)
- Trpm5 channel (Complex from Danio rerio)
- N'-[(e)-(3,4-dimethoxyphenyl)methylidene]-2-(naphthalen-1-yl)acetohydrazide (348 Da, Ligand)
- (25r)-14beta,17beta-spirost-5-en-3beta-ol (414 Da, Ligand)
- Transient receptor potential melastatin 5 (132 kDa, Protein from Danio rerio)
cryo-EM structure of human proton-activated chloride channel PAC at pH 8
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7jna
Q-score: 0.398
Ruan Z, Osei-Owusu J, Du J, Qiu Z, Lu W
Nature (2020) 588 pp. 350-354 [ DOI: doi:10.1038/s41586-020-2875-7 Pubmed: 33149300 ]