Thermophilic eukaryotic 80S ribosome at idle POST state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7olc
Q-score: 0.538
Kisonaite M, Wild K, Lapouge K, Ruppert T, Sinning I
Nat Commun (2022) 13 pp. 476-476 [ DOI: doi:10.1038/s41467-022-27967-9 Pubmed: 35079002 ]
- 60s ribosomal protein l29 (7 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 5s rrna (38 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s0 (31 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Ribosomal protein l19 (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative guanine nucleotide-binding protein (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Habp4_pai-rbp1 domain-containing protein (32 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s15-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l10-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s25 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s30 (6 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s20-like protein (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l44-like protein (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s21-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ubiquitin (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 26s rrna (1 MDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- El39 (6 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative ribosomal protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l16-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s acidic ribosomal protein p0 (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l38-like protein (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l9-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s3-like protein (27 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosome-associated molecular chaperone ssb1 (67 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s10-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l41-a (3 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Us14 (6 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s17-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l13 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l24-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Chaetomium thermophilum 80s ribosome (Cell from Chaetomium thermophilum var. thermophilum DSM 1495)
- 18s rrna (578 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s19-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s27a-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s26 (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s12 (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 5.8s rrna (50 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s2-like protein (28 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein s27-like protein (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Thermophilic eukaryotic 80S ribosome at pe/E (TI)-POST state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7old
Q-score: 0.458
Kisonaite M, Wild K, Lapouge K, Ruppert T, Sinning I
Nat Commun (2022) 13 pp. 476-476 [ DOI: doi:10.1038/s41467-022-27967-9 Pubmed: 35079002 ]
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s0 (31 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s12 (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s21-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s10-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l10-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l44-like protein (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Elongation factor 2 (93 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Zinc ion (65 Da, Ligand)
- Ribosomal protein us14 (6 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s2-like protein (28 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l41-a (3 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s acidic ribosomal protein p0 (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein s27-like protein (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Habp4_pai-rbp1 domain-containing protein (32 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s17-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 18s rrna (578 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s3-like protein (27 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 5.8s rrna (50 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l19 (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l24-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 26s rrna (1 MDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s30 (6 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 5s rrna (38 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l29 (7 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s20-like protein (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s15-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative ribosomal protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s26 (13 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ubiquitin (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative guanine nucleotide-binding protein (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Chaetomium thermophilum 80s ribosome (Cell from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s25 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s27a-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Pe/e trna (24 kDa, RNA from Chaetomium thermophilum var. thermophilum DSM 1495)
- 40s ribosomal protein s19-like protein (17 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Structure of the vertebrate gamma-Tubulin Ring Complex
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6tf9
Q-score: 0.138
Liu P, Zupa E, Neuner A, Bohler A, Loerke J, Flemming D, Ruppert T, Rudack T, Peter C, Spahn C, Gruss OJ, Pfeffer S, Schiebel E
Nature (2020) 578 pp. 467-471 [ Pubmed: 31856152 DOI: doi:10.1038/s41586-019-1896-6 ]
- Belt helix 6 (1 kDa, Protein from Xenopus laevis)
- Belt helices 1,2,3,4 (942 Da, Protein from Xenopus laevis)
- Belt helix 7 (1 kDa, Protein from Xenopus laevis)
- Belt helices 11,12 (1 kDa, Protein from Xenopus laevis)
- Belt helix 16 (1 kDa, Protein from Xenopus laevis)
- Vertebrate gamma-tubulin ring complex (2 MDa, Complex from Xenopus laevis)
- Gamma-tubulin complex component 3 homolog (103 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component 2 (103 kDa, Protein from Xenopus laevis)
- Belt helices 13,14,15 and helix 2 (1 kDa, Protein from Xenopus laevis)
- Tubulin gamma-1 chain (51 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component (117 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component (76 kDa, Protein from Xenopus laevis)
- Gamma tubulin ring protein (184 kDa, Protein from Xenopus laevis)
- Actin, cytoplasmic 1 (41 kDa, Protein from Xenopus laevis)
- Belt helix 5 (2 kDa, Protein from Xenopus laevis)
- Belt helices 8,9,10 (1 kDa, Protein from Xenopus laevis)
- Belt helix 17 (1 kDa, Protein from Xenopus laevis)
- Helix 1 (2 kDa, Protein from Xenopus laevis)