Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6znl
Q-score: 0.352
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin complex (1 MDa, Complex from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 3 (21 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
The shoulder domain of dynactin with underlying Arp1 filament subnits
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
The pointed end complex of dynactin, with accompanying Arp1/actin filament subunits
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
Map of the shoulder domain from dynactin to resolve the upper paddle and hook regions
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
The pointed end complex of dynactin bound to BICDR1
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6znm
Q-score: 0.34
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Dynein-dynactin-bicdr1 complex (1 MDa, Complex)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bicdr1 (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicdr1 (58 kDa, Protein from Mus musculus)
The pointed end complex of dynactin bound to Hook3
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6znn
Q-score: 0.264
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Hook3 (Complex from Homo sapiens)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Putative hook3 coiled coil (5 kDa, Protein from Homo sapiens)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Dynein-dynactin-hook3 complex (Complex)
- Zinc ion (65 Da, Ligand)
The pointed end complex of dynactin with the p150 projection docked
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6zno
Q-score: 0.158
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin 6 (18 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
cryo-EM structure of mTORC1 bound to PRAS40-fused active RagA/C GTPases
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 6sb0
Q-score: 0.237
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science (2019) 366 pp. 203-210 [ Pubmed: 31601764 DOI: doi:10.1126/science.aax3939 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Proline-rich akt1 substrate 1 (27 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Mtorc1 (Complex from Homo sapiens)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Cryoem structure of mtorc1 bound to raga/c complex (1 MDa, Complex)
- Raga/c (Complex from Homo sapiens)
- Mtor,serine/threonine-protein kinase mtor,mtor,serine/threonine-protein kinase mtor (287 kDa, Protein from Homo sapiens)
cryo-EM structure of mTORC1 bound to active RagA/C GTPases
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 6sb2
Q-score: 0.211
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science (2019) 366 pp. 203-210 [ DOI: doi:10.1126/science.aax3939 Pubmed: 31601764 ]
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- Mtorc1 (Complex from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Raga/c (Complex from Homo sapiens)
- Cryo-em structure of mtorc1 bound to active raga/c complex (1 MDa, Complex)
- Mtor,serine/threonine-protein kinase mtor,mtor,serine/threonine-protein kinase mtor (287 kDa, Protein from Homo sapiens)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
Casañal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA
Science (2017) 358 pp. 1056-1059 [ Pubmed: 29074584 DOI: 10.1126/science.aao6535 ]
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