Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6znl
Q-score: 0.352
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Dynactin complex (1 MDa, Complex from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 3 (21 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
The shoulder domain of dynactin with underlying Arp1 filament subnits
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
The pointed end complex of dynactin, with accompanying Arp1/actin filament subunits
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
Map of the shoulder domain from dynactin to resolve the upper paddle and hook regions
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
The pointed end complex of dynactin bound to BICDR1
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6znm
Q-score: 0.34
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin (Complex from Sus scrofa)
- Bicdr1 (Complex from Mus musculus)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicdr1 (58 kDa, Protein from Mus musculus)
- Dynein-dynactin-bicdr1 complex (1 MDa, Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
The pointed end complex of dynactin bound to Hook3
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6znn
Q-score: 0.264
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin (Complex from Sus scrofa)
- Hook3 (Complex from Homo sapiens)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dynein-dynactin-hook3 complex (Complex)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Putative hook3 coiled coil (5 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
The pointed end complex of dynactin with the p150 projection docked
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6zno
Q-score: 0.158
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ Pubmed: 33734450 DOI: doi:10.15252/embj.2020106164 ]
- Dynactin (Complex from Sus scrofa)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Dynactin 6 (18 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
cryo-EM structure of mTORC1 bound to PRAS40-fused active RagA/C GTPases
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 6sb0
Q-score: 0.237
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science (2019) 366 pp. 203-210 [ Pubmed: 31601764 DOI: doi:10.1126/science.aax3939 ]
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Mtor,serine/threonine-protein kinase mtor,mtor,serine/threonine-protein kinase mtor (287 kDa, Protein from Homo sapiens)
- Mtorc1 (Complex from Homo sapiens)
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- Cryoem structure of mtorc1 bound to raga/c complex (1 MDa, Complex)
- Proline-rich akt1 substrate 1 (27 kDa, Protein from Homo sapiens)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Raga/c (Complex from Homo sapiens)
cryo-EM structure of mTORC1 bound to active RagA/C GTPases
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 6sb2
Q-score: 0.211
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science (2019) 366 pp. 203-210 [ Pubmed: 31601764 DOI: doi:10.1126/science.aax3939 ]
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Mtor,serine/threonine-protein kinase mtor,mtor,serine/threonine-protein kinase mtor (287 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Mtorc1 (Complex from Homo sapiens)
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- Cryo-em structure of mtorc1 bound to active raga/c complex (1 MDa, Complex)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Raga/c (Complex from Homo sapiens)
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 6eoj
Q-score: 0.438
Casanal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA
Science (2017) 358 pp. 1056-1059 [ DOI: doi:10.1126/science.aao6535 Pubmed: 29074584 ]
- Zinc ion (65 Da, Ligand)
- Mrna 3'-end-processing protein yth1 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Polyadenylation factor subunit 2,polyadenylation factor subunit 2 (53 kDa, Protein from Saccharomyces cerevisiae S288c)
- Complex of cft1, yth1, pfs2 and fip1 (270 kDa, Complex from SaccharSaccharomyces cerevisiae (strain ATCC 204508 / S288c)omyces)
- Protein cft1 (153 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))