Human leukocyte antigen bound by two alloreactive antibody Fabs
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8t6m
Q-score: 0.498
Killian JT, King RG, Kizziah JL, Fucile CF, Diaz-Avalos R, Qiu S, Silva-Sanchez A, Mousseau BJ, Macon KJ, Callahan AR, Yang G, Hossain ME, Akther J, Houp JA, Rosenblum FD, Porrett PM, Ong SC, Kumar V, Mobley JA, Saphire EO, Kearney JF, Randall TD, Rosenberg AF, Green TJ, Lund FE
To Be Published
- L02/m07/a*01:01 complex (Complex from Homo sapiens)
- Jtk191b_l02_light (23 kDa, Protein from Homo sapiens)
- Jtk191b_m07_light (23 kDa, Protein from Homo sapiens)
- Beta-2-microglobulin (11 kDa, Protein from Homo sapiens)
- Jtk191b_l02_fab (23 kDa, Protein from Homo sapiens)
- Mhc class i antigen (20 kDa, Protein from Homo sapiens)
- Jtk191b_m07_fab (24 kDa, Protein from Homo sapiens)
- Hla_a0101_peptide (1 kDa, Protein from Homo sapiens)
KZ52 Fab fragment bound to Ebola GP, subtomogram average from a tight mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293- [ DOI: doi:10.1038/s41467-025-56452-2 ]
KZ52 Fab fragment bound to Ebola GP, subtomogram average from a cylinder mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293- [ DOI: doi:10.1038/s41467-025-56452-2 ]
3A6 Fab fragment bound to Ebola GP, subtomogram average from a cylinder mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293- [ DOI: doi:10.1038/s41467-025-56452-2 ]
3A6 and KZ52 Fab fragments bound to Ebola GP, subtomogram average from a tight mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293- [ DOI: doi:10.1038/s41467-025-56452-2 ]
3A6 and KZ52 Fab fragments bound to Ebola GP, subtomogram average from a cylinder mask
Hastie KM, Salie ZL, Ke Z, Halfmann PJ, DeWald LE, McArdle S, Grinyo A, Davidson E, Schendel SL, Hariharan C, Norris MJ, Yu X, Chennareddy C, Xiong X, Heinrich M, Holbrook MR, Doranz B, Crozier I, Kawaoka Y, Branco LM, Kuhn JH, Briggs JAG, Worwa G, Davis CW, Ahmed R, Saphire EO
Nat Commun (2025) 16 pp. 1293- [ DOI: doi:10.1038/s41467-025-56452-2 ]
HCMV gH/UL116/UL141 3-mer complex, ectodomain
Resolution: 3.51 Å
EM Method: Single-particle
Fitted PDBs: 9dix
Q-score: 0.432
Norris MJ, Henderson LA, Siddiquey MNA, Yin J, Yoo K, Brunel S, Saphire EO, Benedict CA, Kamil JP
bioRxiv (2024) [ DOI: doi:10.1101/2024.10.13.617647 Pubmed: 39416215 ]
- Protein ul141 (31 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (77 kDa, Protein from Human betaherpesvirus 5)
- Hcmv gh/ul116/ul141 3-mer complex (Complex from Human betaherpesvirus 5)
- Ul116 (35 kDa, Protein from Human betaherpesvirus 5)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Local Cryo-EM structure of HCMV gH/UL116 interaction
Resolution: 5.36 Å
EM Method: Single-particle
Fitted PDBs: 9diy
Q-score: 0.218
Norris MJ, Henderson LA, Siddiquey MNA, Yin J, Yoo K, Brunel S, Saphire EO, Benedict CA, Kamil JP
bioRxiv (2024) [ DOI: doi:10.1101/2024.10.13.617647 Pubmed: 39416215 ]
- Protein ul141 (31 kDa, Protein from Human betaherpesvirus 5)
- Envelope glycoprotein h (77 kDa, Protein from Human betaherpesvirus 5)
- Ul116 (35 kDa, Protein from Human betaherpesvirus 5)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Local structure of gh/ul116 interaction (Complex from Human betaherpesvirus 5)
Intracellular cryo-tomography structure of EBOV nucleocapsid at 8.9 Angstrom
Resolution: 8.9 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8usn
Q-score: 0.251
Watanabe R, Zyla D, Parekh D, Hong C, Jones Y, Schendel SL, Wan W, Castillon G, Saphire EO
Cell (2024) 187 pp. 5587-5603.e19 [ Pubmed: 39293445 DOI: doi:10.1016/j.cell.2024.08.044 ]
- Complex (Virus)
- Outer np with vp35 np-binding peptide (Complex from Ebola virus - Mayinga, Zaire, 1976)
- Rna (5'-r(*ap*ap*ap*ap*ap*a)-3') (1 kDa, RNA from Zaire)
- Vp24 second layer of nucleocapsid (Complex from Ebola virus - Mayinga, Zaire, 1976)
- Membrane-associated protein vp24 (28 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Nucleoprotein (83 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Polymerase cofactor vp35 (37 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Np core with rna (Complex from Homo sapiens)
- Vp35 ctd (Complex from Ebola virus - Mayinga, Zaire, 1976)
Watanabe R, Zyla D, Parekh D, Hong C, Jones Y, Schendel SL, Wan W, Castillon G, Saphire EO
Cell (2024) 187 pp. 5587-5603.e19 [ Pubmed: 39293445 DOI: doi:10.1016/j.cell.2024.08.044 ]