Cryo-EM structure of SAVED-Lon protease CCaCalpL filament bound to A4p
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 9eyi
Q-score: 0.53
Smalakyte D, Ruksenaite A, Sasnauskas G, Tamulaitiene G, Tamulaitis G
Mol Cell (2024) 84 pp. 4239-4255.e8 [ Pubmed: 39362215 DOI: doi:10.1016/j.molcel.2024.09.002 ]
- Filament fragment of a4p bound ccacalpl (Complex)
- A4p (Complex from synthetic construct)
- Ccacalpl (Complex from Candidatus Cloacimonas acidaminovorans)
- A4p (5'-r(*ap*ap*ap*ap)-3') (1 kDa, RNA from synthetic construct)
- Smods-associated and fused to various effectors domain-containing protein (63 kDa, Protein from Candidatus Cloacimonas acidaminovorans)
Cryo-EM structure of SAVED-Lon protease CCaCalpL filament bound to cA4
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 9eyj
Q-score: 0.475
Smalakyte D, Ruksenaite A, Sasnauskas G, Tamulaitiene G, Tamulaitis G
Mol Cell (2024) 84 pp. 4239-4255.e8 [ Pubmed: 39362215 DOI: doi:10.1016/j.molcel.2024.09.002 ]
- Ca4 (1 kDa, RNA from synthetic construct)
- Filament fragment of ca4 bound ccacalpl (Complex)
- Ccacalpl (Complex from Candidatus Cloacimonas acidaminovorans)
- Smods-associated and fused to various effectors domain-containing protein (63 kDa, Protein from Candidatus Cloacimonas acidaminovorans)
- Ca4 (Complex from synthetic construct)
Cryo-EM structure of SAVED-Lon protease CCaCalpL filament bound to A4>p
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 9eyk
Q-score: 0.466
Smalakyte D, Ruksenaite A, Sasnauskas G, Tamulaitiene G, Tamulaitis G
Mol Cell (2024) 84 pp. 4239-4255.e8 [ Pubmed: 39362215 DOI: doi:10.1016/j.molcel.2024.09.002 ]
- Filament fragment of a4>p bound ccacalpl (Complex)
- A4>p (5'-r(*ap*ap*ap*(a23))-3') (1 kDa, RNA from synthetic construct)
- Ccacalpl (Complex from Candidatus Cloacimonas acidaminovorans)
- Smods-associated and fused to various effectors domain-containing protein (63 kDa, Protein from Candidatus Cloacimonas acidaminovorans)
- A4>p (Complex from synthetic construct)
Sasnauskas G, Gaizauskaite U, Tamulaitiene G
To Be Published
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Integration target, chain j (14 kDa, DNA from synthetic construct)
- Cas1-cas2 crispr integrase bound to prespacer and target dna, streptococcus thermophilus dgcc 7710 crispr3 system (Complex from Streptococcus thermophilus DGCC 7710)
- Magnesium ion (24 Da, Ligand)
- Integration target, chain i (14 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Prespacer dna, chain g (8 kDa, DNA from synthetic construct)
- Prespacer dna, chain h (7 kDa, DNA from synthetic construct)
Cas9 bound to cognate DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8pj9
Q-score: 0.479
Sasnauskas G, Gaizauskaite U, Tamulaitiene G
To Be Published
- Dna oligoduplex, target strand, chains d,e (9 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9 (162 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Crrna, chain b and tracrrna, chain c (Complex from Streptococcus thermophilus DGCC 7710)
- Dna oligoduplex, non-target strand, chain f (3 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna oligoduplex, target strand, chains d,e and dna oligoduplex, non-target strand, chain f (Complex from synthetic construct)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus DGCC 7710)
- Complex of cas9 bound to crrna:tracrrna and to a cognate dna duplex (Complex)
- Crrna, chain b (13 kDa, RNA from Streptococcus thermophilus DGCC 7710)
- Tracrrna, chain c (24 kDa, RNA from Streptococcus thermophilus DGCC 7710)
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8pk1
Q-score: 0.463
Sasnauskas G, Gaizauskaite U, Tamulaitiene G
To Be Published
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Cas1-cas2 crispr integrase-prespacer dna complex (Complex from Streptococcus thermophilus DGCC 7710)
- Magnesium ion (24 Da, Ligand)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Chains: g,j (7 kDa, DNA from synthetic construct)
- Chains: h,k (8 kDa, DNA from synthetic construct)
Helical structure of BcThsA in complex with 1''-3'gcADPR
Resolution: 2.96 Å
EM Method: Helical reconstruction
Fitted PDBs: 8bto
Q-score: 0.535
Tamulaitiene G, Sabonis D, Sasnauskas G, Ruksenaite A, Silanskas A, Avraham C, Ofir G, Sorek R, Zaremba M, Siksnys V
Nature (2024) 627 pp. 431-436 [ DOI: doi:10.1038/s41586-024-07092-x Pubmed: 38383786 ]
- Nad(+) hydrolase thsa (55 kDa, Protein from Bacillus cereus MSX-D12)
- (2r,3r,3as,5s,6r,7s,8r,11r,13s,15ar)-2-(6-amino-9h-purin-9-yl)-3,6,7,11,13-pentahydroxyoctahydro-2h,5h,11h,13h-5,8-epoxy-11lambda~5~,13lambda~5~-furo[2,3-g][1,3,5,9,2,4]tetraoxadiphosphacyclotetradecine-11,13-dione (541 Da, Ligand)
- Bcthsa in complex with 1''-3'gcadpr (Complex from Bacillus cereus)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Helical structure of BcThsA in complex with 1''-3'gc(etheno)ADPR
Resolution: 2.75 Å
EM Method: Helical reconstruction
Fitted PDBs: 8btp
Q-score: 0.577
Tamulaitiene G, Sabonis D, Sasnauskas G, Ruksenaite A, Silanskas A, Avraham C, Ofir G, Sorek R, Zaremba M, Siksnys V
Nature (2024) 627 pp. 431-436 [ DOI: doi:10.1038/s41586-024-07092-x Pubmed: 38383786 ]
- Nad(+) hydrolase thsa (55 kDa, Protein from Bacillus cereus MSX-D12)
- (1s,3s,4r,5r,7r,15r,16s,17r)-5-imidazo[2,1-f]purin-3-yl-10,12-bis(oxidanyl)-10,12-bis(oxidanylidene)-2,6,9,11,13,18-hexaoxa-10$l^{5},12$l^{5}-diphosphatricyclo[13.2.1.0^{3,7}]octadecane-4,16,17-triol (565 Da, Ligand)
- Bcthsa in complex with 1''-3'gc(etheno)adpr (Complex from Bacillus cereus)
- Etheno-nad (687 Da, Ligand)
Cryo-EM structure of the Cas12m-crRNA-target DNA complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8pm4
Q-score: 0.542
Bigelyte G, Duchovska B, Zedaveinyte R, Sasnauskas G, Sinkunas T, Dalgediene I, Tamulaitiene G, Silanskas A, Kazlauskas D, Valancauskas L, Madariaga-Marcos J, Seidel R, Siksnys V, Karvelis T
Nucleic Acids Res (2024) 52 pp. 3234-3248 [ Pubmed: 38261981 DOI: doi:10.1093/nar/gkae016 ]
- Crrna, chain b (18 kDa, RNA from Gordonia otitidis NBRC 100426)
- Cas12m protein in complex with crrna and cognate dna, gordonia otitidis nbrc 100426 system (Complex from Gordonia otitidis NBRC 100426)
- Transposase (68 kDa, Protein from Gordonia otitidis NBRC 100426)
- Dna oligoduplex, non-target strand, chain d (13 kDa, DNA from synthetic construct)
- Dna oligoduplex, target strand, chain c (13 kDa, DNA from synthetic construct)
Mogila I, Tamulaitiene G, Keda K, Timinskas A, Ruksenaite A, Sasnauskas G, Venclovas C, Siksnys V, Tamulaitis G
Science (2023) 382 pp. 1036-1041 [ DOI: doi:10.1126/science.adj2107 Pubmed: 38033086 ]