Cryo-EM structure of the Cas12m-crRNA-target DNA complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8pm4
Q-score: 0.542
Bigelyte G, Duchovska B, Zedaveinyte R, Sasnauskas G, Sinkunas T, Dalgediene I, Tamulaitiene G, Silanskas A, Kazlauskas D, Valancauskas L, Madariaga-Marcos J, Seidel R, Siksnys V, Karvelis T
Nucleic Acids Res (2024) 52 pp. 3234-3248 [ DOI: doi:10.1093/nar/gkae016 Pubmed: 38261981 ]
- Cas12m protein in complex with crrna and cognate dna, gordonia otitidis nbrc 100426 system (Complex from Gordonia otitidis NBRC 100426)
- Dna oligoduplex, non-target strand, chain d (13 kDa, DNA from synthetic construct)
- Dna oligoduplex, target strand, chain c (13 kDa, DNA from synthetic construct)
- Transposase (68 kDa, Protein from Gordonia otitidis NBRC 100426)
- Crrna, chain b (18 kDa, RNA from Gordonia otitidis NBRC 100426)
ISDra2 TnpB in complex with reRNA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8bf8
Q-score: 0.497
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V
Nature (2023) 616 pp. 384-389 [ DOI: doi:10.1038/s41586-023-05826-x Pubmed: 37020015 ]
- Deinococcus radiodurans r1 chromosome 1 (48 kDa, RNA from Deinococcus radiodurans R1)
- Isdra2 tnpb binary complex (100 kDa, Complex from Deinococcus radiodurans)
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1)
ISDra2 TnpB in complex with reRNA and cognate DNA, conformation 2 (RuvC domain unresolved)
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8ex9
Q-score: 0.45
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V
Nature (2023) 616 pp. 384-389 [ DOI: doi:10.1038/s41586-023-05826-x Pubmed: 37020015 ]
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Rna (150-mer) (48 kDa, RNA from Deinococcus radiodurans R1)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Isdra2 tnpb in complex with rerna and cognate dna (Complex from Deinococcus radiodurans R1)
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1)
ISDra2 TnpB in complex with reRNA and cognate DNA, conformation 1 (RuvC domain resolved)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8exa
Q-score: 0.441
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V
Nature (2023) 616 pp. 384-389 [ DOI: doi:10.1038/s41586-023-05826-x Pubmed: 37020015 ]
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Rna (150-mer) (48 kDa, RNA from Deinococcus radiodurans R1)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Isdra2 tnpb in complex with rerna and cognate dna (Complex from Deinococcus radiodurans R1)
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1)