10E8-GT10.2 immunogen in complex with human Fab 10E8 and mouse Fab W6-10
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8sx3
Q-score: 0.378
Schiffner T, Phung I, Ray R, Irimia A, Tian M, Swanson O, Lee JH, Lee CD, Marina-Zarate E, Cho SY, Huang J, Ozorowski G, Skog PD, Serra AM, Rantalainen K, Allen JD, Baboo S, Rodriguez OL, Himansu S, Zhou J, Hurtado J, Flynn CT, McKenney K, Havenar-Daughton C, Saha S, Shields K, Schulze S, Smith ML, Liang CH, Toy L, Pecetta S, Lin YC, Willis JR, Sesterhenn F, Kulp DW, Hu X, Cottrell CA, Zhou X, Ruiz J, Wang X, Nair U, Kirsch KH, Cheng HL, Davis J, Kalyuzhniy O, Liguori A, Diedrich JK, Ngo JT, Lewis V, Phelps N, Tingle RD, Spencer S, Georgeson E, Adachi Y, Kubitz M, Eskandarzadeh S, Elsliger MA, Amara RR, Landais E, Briney B, Burton DR, Carnathan DG, Silvestri G, Watson CT, Yates 3rd JR, Paulson JC, Crispin M, Grigoryan G, Ward AB, Sok D, Alt FW, Wilson IA, Batista FD, Crotty S, Schief WR
Nat Immunol (2024) 25 pp. 1073-1082 [ DOI: doi:10.1038/s41590-024-01833-w Pubmed: 38816615 ]
- W6-10 mouse fab heavy chain, w6-10 mouse light chain (Complex from Mus musculus)
- 10e8-gt10.2 immunogen in complex with human fab 10e8 and mouse fab w6-10 (Complex)
- 10e8-gt10.2 immunogen (Complex from synthetic construct)
- 10e8 light chain (22 kDa, Protein from Homo sapiens)
- W6-10 mouse fab heavy chain (23 kDa, Protein from Mus musculus)
- W6-10 mouse light chain (23 kDa, Protein from Mus musculus)
- 10e8 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 10e8-gt10.2 immunogen (21 kDa, Protein from synthetic construct)
- 10e8 fab heavy chain, 10e8 light chain (Complex from Homo sapiens)
MD65 N332-GT5 SOSIP in complex with RM_N332_03 Fab and RM20A3 Fab
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8t49
Q-score: 0.495
Steichen JM, Phung I, Salcedo E, Ozorowski G, Willis JR, Baboo S, Liguori A, Cottrell CA, Torres JL, Madden PJ, Ma KM, Sutton HJ, Lee JH, Kalyuzhniy O, Allen JD, Rodriguez OL, Adachi Y, Mullen TM, Georgeson E, Kubitz M, Burns A, Barman S, Mopuri R, Metz A, Altheide TK, Diedrich JK, Saha S, Shields K, Schultze SE, Smith ML, Schiffner T, Burton DR, Watson CT, Bosinger SE, Crispin M, Yates 3rd JR, Paulson JC, Ward AB, Sok D, Crotty S, Schief WR
Science (2024) 384 pp. eadj8321-eadj8321 [ Pubmed: 38753769 DOI: doi:10.1126/science.adj8321 ]
- Md65 n332-gt5 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Rm_n332_03 heavy chain fv (14 kDa, Protein from Macaca mulatta)
- Rm20a3 heavy chain fv (13 kDa, Protein from Macaca mulatta)
- Rm_n332_03 light chain fv (11 kDa, Protein from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rm20a3 light chain fv (13 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Md65 n332-gt5 sosip in complex with rm_n332_03 fab and rm20a3 fab (Complex from Macaca mulatta)
MD65 N332-GT5 SOSIP in complex with RM_N332_36 Fab and RM20A3 Fab
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8t4a
Q-score: 0.466
Steichen JM, Phung I, Salcedo E, Ozorowski G, Willis JR, Baboo S, Liguori A, Cottrell CA, Torres JL, Madden PJ, Ma KM, Sutton HJ, Lee JH, Kalyuzhniy O, Allen JD, Rodriguez OL, Adachi Y, Mullen TM, Georgeson E, Kubitz M, Burns A, Barman S, Mopuri R, Metz A, Altheide TK, Diedrich JK, Saha S, Shields K, Schultze SE, Smith ML, Schiffner T, Burton DR, Watson CT, Bosinger SE, Crispin M, Yates 3rd JR, Paulson JC, Ward AB, Sok D, Crotty S, Schief WR
Science (2024) 384 pp. eadj8321-eadj8321 [ Pubmed: 38753769 DOI: doi:10.1126/science.adj8321 ]
- Md65 n332-gt5 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Md65 n332-gt5 sosip in complex with rm_n332_36 fab and rm20a3 fab (Complex from Macaca mulatta)
- Rm_n332_36 light chain fv (11 kDa, Protein from Macaca mulatta)
- Rm20a3 heavy chain fv (13 kDa, Protein from Macaca mulatta)
- Rm_n332_36 heavy chain fv (14 kDa, Protein from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rm20a3 light chain fv (13 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
MD65 N332-GT5 SOSIP in complex with RM_N332_32 Fab and RM20A3
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8t4b
Q-score: 0.483
Steichen JM, Phung I, Salcedo E, Ozorowski G, Willis JR, Baboo S, Liguori A, Cottrell CA, Torres JL, Madden PJ, Ma KM, Sutton HJ, Lee JH, Kalyuzhniy O, Allen JD, Rodriguez OL, Adachi Y, Mullen TM, Georgeson E, Kubitz M, Burns A, Barman S, Mopuri R, Metz A, Altheide TK, Diedrich JK, Saha S, Shields K, Schultze SE, Smith ML, Schiffner T, Burton DR, Watson CT, Bosinger SE, Crispin M, Yates 3rd JR, Paulson JC, Ward AB, Sok D, Crotty S, Schief WR
Science (2024) 384 pp. eadj8321-eadj8321 [ Pubmed: 38753769 DOI: doi:10.1126/science.adj8321 ]
- Md65 n332-gt5 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Rm_n332_32 heavy chain fv (13 kDa, Protein from Macaca mulatta)
- Rm_n332_32 light chain fv (11 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Rm20a3 heavy chain fv (13 kDa, Protein from Macaca mulatta)
- Rm20a3 light chain fv (13 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip in complex with rm_n332_32 fab and rm20a3 fab (Complex from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
MD65 N332-GT5 SOSIP in complex with RM_N332_08 Fab and RM20A3 Fab
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8t4d
Q-score: 0.509
Steichen JM, Phung I, Salcedo E, Ozorowski G, Willis JR, Baboo S, Liguori A, Cottrell CA, Torres JL, Madden PJ, Ma KM, Sutton HJ, Lee JH, Kalyuzhniy O, Allen JD, Rodriguez OL, Adachi Y, Mullen TM, Georgeson E, Kubitz M, Burns A, Barman S, Mopuri R, Metz A, Altheide TK, Diedrich JK, Saha S, Shields K, Schultze SE, Smith ML, Schiffner T, Burton DR, Watson CT, Bosinger SE, Crispin M, Yates 3rd JR, Paulson JC, Ward AB, Sok D, Crotty S, Schief WR
Science (2024) 384 pp. eadj8321-eadj8321 [ Pubmed: 38753769 DOI: doi:10.1126/science.adj8321 ]
- Md65 n332-gt5 sosip in complex with rm_n332_08 fab and rm20a3 fab (Complex from Macaca mulatta)
- Md65 n332-gt5 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Rm_n332_08 light chain fv (12 kDa, Protein from Macaca mulatta)
- Rm20a3 heavy chain fv (13 kDa, Protein from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rm20a3 light chain fv (13 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Rm_n332_08 heavy chain fv (14 kDa, Protein from Macaca mulatta)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8t4k
Q-score: 0.62
Steichen JM, Phung I, Salcedo E, Ozorowski G, Willis JR, Baboo S, Liguori A, Cottrell CA, Torres JL, Madden PJ, Ma KM, Sutton HJ, Lee JH, Kalyuzhniy O, Allen JD, Rodriguez OL, Adachi Y, Mullen TM, Georgeson E, Kubitz M, Burns A, Barman S, Mopuri R, Metz A, Altheide TK, Diedrich JK, Saha S, Shields K, Schultze SE, Smith ML, Schiffner T, Burton DR, Watson CT, Bosinger SE, Crispin M, Yates 3rd JR, Paulson JC, Ward AB, Sok D, Crotty S, Schief WR
Science (2024) 384 pp. eadj8321-eadj8321 [ Pubmed: 38753769 DOI: doi:10.1126/science.adj8321 ]
- Md64 n332-gt5 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Md64 n332-gt5 sosip (Complex from Human immunodeficiency virus 1)
- Md64 n332-gt5 sosip gp41 (18 kDa, Protein from Human immunodeficiency virus 1)
MD65 N332-GT5 SOSIP in complex with RM_N332_07 Fab and RM20A3 Fab
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8t4l
Q-score: 0.476
Steichen JM, Phung I, Salcedo E, Ozorowski G, Willis JR, Baboo S, Liguori A, Cottrell CA, Torres JL, Madden PJ, Ma KM, Sutton HJ, Lee JH, Kalyuzhniy O, Allen JD, Rodriguez OL, Adachi Y, Mullen TM, Georgeson E, Kubitz M, Burns A, Barman S, Mopuri R, Metz A, Altheide TK, Diedrich JK, Saha S, Shields K, Schultze SE, Smith ML, Schiffner T, Burton DR, Watson CT, Bosinger SE, Crispin M, Yates 3rd JR, Paulson JC, Ward AB, Sok D, Crotty S, Schief WR
Science (2024) 384 pp. eadj8321-eadj8321 [ Pubmed: 38753769 DOI: doi:10.1126/science.adj8321 ]
- Md65 n332-gt5 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Rm_n332_07 light chain fv (11 kDa, Protein from Macaca mulatta)
- Rm20a3 heavy chain fv (13 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip in complex with rm_n332_07 fab and rm20a3 fab (Complex from Macaca mulatta)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rm20a3 light chain fv (13 kDa, Protein from Macaca mulatta)
- Md65 n332-gt5 sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Rm_n332_07 heavy chain fv (14 kDa, Protein from Macaca mulatta)
Negative Stain EM reconstruction of ApexGT3 in complex with two copies of PG9 Fab
Willis JR, Berndsen ZT, Ma KM, Steichen JM, Schiffner T, Landais E, Liguori A, Kalyuzhniy O, Allen JD, Baboo S, Omorodion O, Diedrich JK, Hu X, Georgeson E, Phelps N, Eskandarzadeh S, Groschel B, Kubitz M, Adachi Y, Mullin TM, Alavi NB, Falcone S, Himansu S, Carfi A, Wilson IA, Paulson JC, Crispin M, Ward AB, Schief WR
Immunity (2022) 55 pp. 2149-2167.e9 [ Pubmed: 36179689 DOI: doi:10.1016/j.immuni.2022.09.001 ]
HIV-1 Envelope ApexGT2.2MUT in complex with PCT64.LMCA Fab
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7t73
Q-score: 0.388
Willis JR, Berndsen ZT, Ma KM, Steichen JM, Schiffner T, Landais E, Liguori A, Kalyuzhniy O, Allen JD, Baboo S, Omorodion O, Diedrich JK, Hu X, Georgeson E, Phelps N, Eskandarzadeh S, Groschel B, Kubitz M, Adachi Y, Mullin TM, Alavi NB, Falcone S, Himansu S, Carfi A, Wilson IA, Yates 3rd JR, Paulson JC, Crispin M, Ward AB, Schief WR
Immunity (2022) 55 pp. 2149- [ Pubmed: 36179689 DOI: doi:10.1016/j.immuni.2022.09.001 ]
- Hiv envelope apexgt2.2mut gp41 (18 kDa, Protein from Human immunodeficiency virus 1)
- Pct64.lmca fab light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Pct64.lmca fab heavy chain (25 kDa, Protein from Homo sapiens)
- Hiv envelope apexgt2.2mut gp120 (56 kDa, Protein from Human immunodeficiency virus 1)
- Hiv-1 envelope apexgt2.2mut in complex with pct64.lmca fab (Complex from Human immunodeficiency virus 1)
HIV-1 Envelope ApexGT2 in complex with PCT64.35S Fab and RM20A3 Fab
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 7t74
Q-score: 0.476
Willis JR, Berndsen ZT, Ma KM, Steichen JM, Schiffner T, Landais E, Liguori A, Kalyuzhniy O, Allen JD, Baboo S, Omorodion O, Diedrich JK, Hu X, Georgeson E, Phelps N, Eskandarzadeh S, Groschel B, Kubitz M, Adachi Y, Mullin TM, Alavi NB, Falcone S, Himansu S, Carfi A, Wilson IA, Yates 3rd JR, Paulson JC, Crispin M, Ward AB, Schief WR
Immunity (2022) 55 pp. 2149- [ Pubmed: 36179689 DOI: doi:10.1016/j.immuni.2022.09.001 ]
- Hiv envelope apexgt2 gp120 (56 kDa, Protein from Human immunodeficiency virus 1)
- Rm20a3 fab light chain (13 kDa, Protein from Macaca mulatta)
- Rm20a3 fab heavy chain (13 kDa, Protein from Macaca mulatta)
- Hiv envelope apexgt2 gp41 (18 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hiv-1 envelope apexgt2 in complex with pct64.35 fab and rm20a3 fab (Complex from Human immunodeficiency virus 1)
- Pct64.35s fab heavy chain (14 kDa, Protein from Homo sapiens)
- Pct64.35s fab light chain (11 kDa, Protein from Homo sapiens)