Negative stain EM map of MERS 2 in complex with G2 Fab
Wang N, Rosen O, Wang L, Turner HL, Stevens LJ, Corbett KS, Bowman CA, Pallesen J, Shi W, Zhang Y, Leung K, Kirchdoerfer RN, Becker MM, Denison MR, Chappell JD, Ward AB, Graham BS, McLellan JS
Cell Rep (2019) 28 pp. 3395-3405.e6 [ Pubmed: 31553909 DOI: doi:10.1016/j.celrep.2019.08.052 ]
MERS S0 trimer in complex with antibody G2
Resolution: 4.19 Å
EM Method: Single-particle
Fitted PDBs: 6pz8
Q-score: 0.133
Wang N, Rosen O, Wang L, Turner HL, Stevens LJ, Corbett KS, Bowman CA, Pallesen J, Shi W, Zhang Y, Leung K, Kirchdoerfer RN, Becker MM, Denison MR, Chappell JD, Ward AB, Graham BS, McLellan JS
Cell Rep (2019) 28 pp. 3395-3405.e6 [ Pubmed: 31553909 DOI: doi:10.1016/j.celrep.2019.08.052 ]
- G2 light chain (23 kDa, Protein from Mus musculus)
- Antibody g2 (Complex from Mus musculus)
- S protein (51 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Mers s0 ectodomain trimer in complex with antibody g2 (Complex)
- G2 heavy chain (24 kDa, Protein from Mus musculus)
- Mers s0 ectodomain trimer (Complex from Middle East respiratory syndrome-related coronavirus)
- S protein (80 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5w9h
Q-score: 0.313
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vl (23 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Mers s (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- G4 vh (25 kDa, Protein from Mus musculus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5w9i
Q-score: 0.414
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vl (23 kDa, Protein from Mus musculus)
- Spike glycoprotein (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- G4 vh (25 kDa, Protein from Mus musculus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 5w9j
Q-score: 0.237
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vh (25 kDa, Protein from Mus musculus)
- G4 vl (23 kDa, Protein from Mus musculus)
- Spike glycoprotein (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5w9k
Q-score: 0.242
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vh (25 kDa, Protein from Mus musculus)
- G4 vl (23 kDa, Protein from Mus musculus)
- Spike glycoprotein (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 5w9l
Q-score: 0.237
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vh (25 kDa, Protein from Mus musculus)
- G4 vl (23 kDa, Protein from Mus musculus)
- Spike glycoprotein (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 5w9m
Q-score: 0.252
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vh (25 kDa, Protein from Mus musculus)
- G4 vl (23 kDa, Protein from Mus musculus)
- Spike glycoprotein (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 5.0 Å
EM Method: Single-particle
Fitted PDBs: 5w9n
Q-score: 0.214
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- Mers s (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- G4 vh (25 kDa, Protein from Mus musculus)
- G4 vl (23 kDa, Protein from Mus musculus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)
MERS S ectodomain trimer in complex with Fab of neutralizing antibody G4
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5w9o
Q-score: 0.265
Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS
PNAS (2017) 114 pp. E7348-E7357 [ Pubmed: 28807998 DOI: doi:10.1073/pnas.1707304114 ]
- G4 vh (25 kDa, Protein from Mus musculus)
- G4 vl (23 kDa, Protein from Mus musculus)
- Spike glycoprotein (146 kDa, Protein from Middle East respiratory syndrome-related coronavirus)
- Mers s ectodomain trimer in complex with fab of neutralizing antibody g4 (600 kDa, Complex from Middle East respiratory syndrome-related coronavirus)