Cryo-EM structure of the human BRISC dimer complex bound to compound FX-171-C
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8pvy
Q-score: 0.332
Chandler F, Reddy P, Bhutda S, Ross R, Walden M, Walker K, Cassel J, Prakesch M, Datti A, Campbell L, Foglizzo M, Bell L, Ault J, Al-awar R, Calabrese A, Sicheri F, Del Galdo F, Salvino J, Greenberg R, Zeqiraj E
To Be Published
- Zinc ion (65 Da, Ligand)
- Brisc and brca1-a complex member 1 (29 kDa, Protein from Homo sapiens)
- N-[(1r,5s)-3-azabicyclo[3.1.0]hexan-6-yl]-1-[2,6-bis(chloranyl)phenyl]carbonyl-4-[[2,6-bis(chloranyl)phenyl]carbonylamino]pyrazole-3-carboxamide (553 Da, Ligand)
- Brisc and brca1-a complex member 2 (43 kDa, Protein from Homo sapiens)
- Brisc dimer in complex with inhibitor fx-171-c (654 kDa, Complex from Homo sapiens)
- Lys-63-specific deubiquitinase brcc36 (35 kDa, Protein from Homo sapiens)
- Brisc complex subunit abraxas 2 (31 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human BRISC dimer complex bound to compound JMS-175-2
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8py2
Q-score: 0.281
Chandler F, Reddy P, Bhutda S, Ross R, Walden M, Walker K, Cassel J, Prakesch M, Campbell L, Foglizzo M, Bell L, Ault J, Al-awar R, Calabrese A, Sicheri F, Del Galdo F, Salvino J, Greenberg R, Zeqiraj E
To Be Published
- Brisc and brca1-a complex member 2 (43 kDa, Protein from Homo sapiens)
- Brisc dimer in complex with inhibitor jms-175-2 (654 kDa, Complex from Homo sapiens)
- Brisc and brca1-a complex member 1 (29 kDa, Protein from Homo sapiens)
- Lys-63-specific deubiquitinase brcc36 (36 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Brisc complex subunit abraxas 2 (31 kDa, Protein from Homo sapiens)
- 1-[2,6-bis(chloranyl)phenyl]carbonyl-4-[[2,6-bis(chloranyl)phenyl]carbonylamino]-n-piperidin-4-yl-pyrazole-3-carboxamide (555 Da, Ligand)
Structure of the KEOPS complex (Cgi121/Bud32/Kae1/Pcc1)
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8unk
Q-score: 0.421
Ona Chuquimarca SM, Beenstock J, Daou S, Porat J, Keszei AFA, Yin JZ, Beschauner T, Bayfield MA, Mazhab-Jafari MT, Sicheri F
Nat Commun (2024) 15 pp. 10633-10633 [ DOI: doi:10.1038/s41467-024-54787-w Pubmed: 39639027 ]
- Regulatory protein cgi121 (16 kDa, Protein from Methanocaldococcus jannaschii)
- Bud32 (Complex from Methanocaldococcus jannaschii)
- Probable bifunctional trna threonylcarbamoyladenosine biosynthesis protein (35 kDa, Protein from Methanocaldococcus jannaschii)
- Structure of the keops complex (cgi121/bud32/kae1/pcc1) (Complex from Methanocaldococcus jannaschii)
- Pcc1 (Complex from Pyrococcus furiosus)
- Keops complex pcc1-like subunit (9 kDa, Protein from Pyrococcus furiosus)
- Cgi121 (Complex from Methanocaldococcus jannaschii)
- Kae1 (Complex from Methanocaldococcus jannaschii)
- Probable bifunctional trna threonylcarbamoyladenosine biosynthesis protein (23 kDa, Protein from Methanocaldococcus jannaschii)
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8up5
Q-score: 0.348
Ona Chuquimarca SM, Beenstock J, Daou S, Porat J, Keszei AFA, Yin JZ, Beschauner T, Bayfield MA, Mazhab-Jafari MT, Sicheri F
Nat Commun (2024) 15 pp. 10633-10633 [ Pubmed: 39639027 DOI: doi:10.1038/s41467-024-54787-w ]
- Structure of the keops complex (cgi121/bud32/kae1/pcc1) bound to trna in its native-like conformation. (Complex from Methanocaldococcus jannaschii)
- Probable bifunctional trna threonylcarbamoyladenosine biosynthesis protein (35 kDa, Protein from Methanocaldococcus jannaschii)
- Keops complex subunit pcc1 (19 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Trna (Complex from Methanocaldococcus jannaschii)
- Probable bifunctional trna threonylcarbamoyladenosine biosynthesis protein (23 kDa, Protein from Methanocaldococcus jannaschii)
- Trna (24 kDa, RNA from Methanocaldococcus jannaschii)
- Cgi121 (Complex from Methanocaldococcus jannaschii)
- Bud32 (Complex from Methanocaldococcus jannaschii)
- Kae1 (Complex from Methanocaldococcus jannaschii)
- Pcc1 (Complex from Pyrococcus furiosus)
- Regulatory protein cgi121 (16 kDa, Protein from Methanocaldococcus jannaschii)
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 9d85
Q-score: 0.156
Ona Chuquimarca SM, Beenstock J, Daou S, Porat J, Keszei AFA, Yin JZ, Beschauner T, Bayfield MA, Mazhab-Jafari MT, Sicheri F
Nat Commun (2024) 15 pp. 10633-10633 [ DOI: doi:10.1038/s41467-024-54787-w Pubmed: 39639027 ]
- Bud32 (Complex from Methanocaldococcus jannaschii)
- Probable bifunctional trna threonylcarbamoyladenosine biosynthesis protein (36 kDa, Protein from Methanocaldococcus jannaschii)
- Structure of the keops complex (cgi121/bud32/kae1/pcc1) (Complex from Methanocaldococcus jannaschii)
- Regulatory protein cgi121 (16 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Pcc1 (Complex from Pyrococcus furiosus)
- Cgi121 (Complex from Methanocaldococcus jannaschii)
- Keops complex subunit pcc1 (9 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Kae1 (Complex from Methanocaldococcus jannaschii)
- Trna (24 kDa, RNA from Methanocaldococcus jannaschii)
- Probable bifunctional trna threonylcarbamoyladenosine biosynthesis protein (23 kDa, Protein from Methanocaldococcus jannaschii)
Cryo-EM structure of the inner MKLN1 dimer from an autoinhibited MKLN1 tetramer
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8ttq
Q-score: 0.398
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ Pubmed: 39215025 DOI: doi:10.1038/s41467-024-52009-x ]
Cryo-EM structure of an autoinhibited MKLN1 tetramer
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ Pubmed: 39215025 DOI: doi:10.1038/s41467-024-52009-x ]
Cryo-EM structure of CTLH-MKLN1-FAM72A in complex with UNG2
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ Pubmed: 39215025 DOI: doi:10.1038/s41467-024-52009-x ]
Cryo-EM structure of a FAM72A-MKLN1-RANBP9-TWA1 complex
Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A
Nat Commun (2024) 15 pp. 7541-7541 [ Pubmed: 39215025 DOI: doi:10.1038/s41467-024-52009-x ]
CryoEM structure of the HisRS-like domain of human GCN2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8t7t
Q-score: 0.45
Yin JZ, Keszei AFA, Houliston S, Filandr F, Beenstock J, Daou S, Kitaygorodsky J, Schriemer DC, Mazhab-Jafari MT, Gingras AC, Sicheri F
Structure (2024) 32 pp. 795-811.e6 [ DOI: doi:10.1016/j.str.2024.02.021 Pubmed: 38531363 ]
- Eif-2-alpha kinase gcn2 (187 kDa, Protein from Homo sapiens)
- Dimeric hisrs-like domain of human gcn2 (Complex from Homo sapiens)