Cryo-EM structure of the E. coli BrxX methyltransferase in complex with DNA
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
- Dna (Complex from Escherichia coli)
- Brxx methyltransferase (Complex from Escherichia coli)
- Dna (26-mer) with brex binding site ggtaag (7 kDa, DNA from Escherichia coli)
- Brxx methyltransferase from e. coli brex phage defense system in complex with dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Dna (26-mer) with brex binding site ggtaag (7 kDa, DNA from Escherichia coli)
- Adenine-specific methyltransferase brxx (138 kDa, Protein from Escherichia coli)
- S-adenosylmethionine (398 Da, Ligand)
Focused map for NTD and MTD domains of the phage immunity methyltransferase protein BrxX.
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
Consensus map for the phage immunity methyltransferase protein BrxX
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
Cryo-EM structure of the E. coli BrxX methyltransferase in complex with Ocr
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
- Brxx methyltransferase (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Brxx methyltransferase from e. coli brex phage defense system in complex with ocr (Complex)
- Protein ocr (13 kDa, Protein from Escherichia phage T7)
- Ocr (Complex from Escherichia phage T7)
- Adenine-specific methyltransferase brxx (138 kDa, Protein from Escherichia coli)
- S-adenosylmethionine (398 Da, Ligand)
Cryo-EM structure of the Apo E. coli BrxX methyltransferase
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
Single Particle Cryo-EM of the PARIS Immune complex following release of AriB
Burman NB, Belukhina S, Depardieu F, Wilkinson R, Skutel M, Graham A, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques W, Buyukyoruk M, Rouillon C, Shyrokova O, Kurata T, Hauryliuk V, Severinov K, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A
To Be Published
Image sets:
Structure of the PARIS immune complex with AriB subunits in C3 arrangement.
Burman NB, Belukhina S, Depardieu F, Wilkinson R, Skutel M, Santiago-Frangos A, Graham A, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques W, Buyukyoruk M, Rouillon C, Shyrokova O, Suzuki T, Hauryliuk V, Severinov K, Groseille J, Thierry A, Koszul R, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A
To Be Published
- Aria, abc atpase and sensor of paris immunity (Protein from Escherichia coli B185)
- Arib, duf4435 and toprim nuclease. effector of the paris immune complex. (Protein from Escherichia coli B185)
- Paris immune complex in the assembled state with arib subunits in a c3-symmetric arrangement. (Complex from Escherichia coli B185)
PARIS immune complex with AriB subunits in the trans arrangement.
Burman NB, Belukhina S, Depardieu F, Wilkinson R, Skutel M, Santiago-Frangos A, Graham A, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques W, Buyukyoruk M, Rouillon C, Shyrokova O, Suzuki T, Hauryliuk V, Severinov K, Groseille J, Thierry A, Koszul R, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A
To Be Published
- Arib, duf4435 and toprim containing nuclease, effector of the paris immune system. (Protein from Escherichia coli B185)
- Aria, abc atpase and sensor of paris immunity (Protein from Escherichia coli B185)
- Paris immune complex in the assembled state with arib subunits in a c3-symmetric arrangement. (Complex from Escherichia coli B185)
Map of the AriA homohexamer following release of the AriB effector during PARIS-mediated defense.
Burman NB, Belukhina S, Depardieu F, Wilkinson R, Skutel M, Santiago-Frangos A, Graham A, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques W, Buyukyoruk M, Rouillon C, Shyrokova O, Suzuki T, Hauryliuk V, Severinov K, Groseille J, Thierry A, Koszul R, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A
To Be Published
Asymmetric unit of the PARIS Immune Complex at 3.2 Angstrom Resolution
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8ux9
Q-score: 0.519
Burman NB, Belukhina S, Depardieu F, Wilkinson R, Skutel M, Graham A, Livenskyi A, Chechenina A, Morozova N, Zahl T, Henriques W, Buyukyoruk M, Rouillon C, Shyrokova O, Suzuki T, Hauryliuk V, Severinov K, Groseille J, Thierry A, Koszul R, Tesson F, Bernheim A, Bikard D, Wiedenheft B, Isaev A
To Be Published
- Asymmetric unit of the paris immune complex with aria bound to two molecules of atpgs and inactive arib effector. (Complex from Escherichia coli B185)
- Arib (35 kDa, Protein from Escherichia coli B185)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Aria (53 kDa, Protein from Escherichia coli B185)