Structures of positive allosteric modulator-bound and unbound active human calcium-sensing receptor
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
Proc Natl Acad Sci U S A (2021) 118 [ DOI: 10.1073/pnas.2115849118 Pubmed: 34916296 ]
Image sets:
Structure of negative allosteric modulator-bound inactive human calcium-sensing receptor
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
Proc Natl Acad Sci U S A (2021) 118 [ DOI: 10.1073/pnas.2115849118 Pubmed: 34916296 ]
Image sets:
Structure of positive allosteric modulator-bound active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sil
Q-score: 0.562
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of positive allosteric modulator-free active human calcium-sensing receptor
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sim
Q-score: 0.555
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (99 kDa, Protein from Homo sapiens)
- Cyclomethyltryptophan (216 Da, Ligand)
- Homodimer human calcium sensing receptor (192 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of negative allosteric modulator-bound inactive human calcium-sensing receptor
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 7sin
Q-score: 0.19
Park J, Zuo H, Frangaj A, Fu Z, Yen LY, Zhang Z, Mosyak L, Slavkovich VN, Liu J, Ray KM, Cao B, Vallese F, Geng Y, Chen S, Grassucci R, Dandey VP, Tan YZ, Eng E, Lee Y, Kloss B, Liu Z, Hendrickson WA, Potter CS, Carragher B, Graziano J, Conigrave AD, Frank J, Clarke OB, Fan QR
PNAS (2021) 118 [ Pubmed: 34916296 DOI: doi:10.1073/pnas.2115849118 ]
Structure of human GABA(B) receptor in an inactive state
Park J, Fu Z, Frangaj A, Liu J, Mosyak L, Shen T, Slavkovich VN, Ray KM, Taura J, Cao B, Geng Y, Zuo H, Kou Y, Grassucci R, Chen S, Liu Z, Lin X, Williams JP, Rice WJ, Eng ET, Huang RK, Soni RK, Kloss B, Yu Z, Javitch JA, Hendrickson WA, Slesinger PA, Quick M, Graziano J, Yu H, Fiehn O, Clarke OB, Frank J, Fan QR
Nature (2020) 584 pp. 304-309 [ DOI: 10.1038/s41586-020-2452-0 Pubmed: 32581365 ]
Image sets:
Structure of human GABA(B) receptor in an inactive state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6wiv
Q-score: 0.486
Park J, Fu Z, Frangaj A, Liu J, Mosyak L, Shen T, Slavkovich VN, Ray KM, Taura J, Cao B, Geng Y, Zuo H, Kou Y, Grassucci R, Chen S, Liu Z, Lin X, Williams JP, Rice WJ, Eng ET, Huang RK, Soni RK, Kloss B, Yu Z, Javitch JA, Hendrickson WA, Slesinger PA, Quick M, Graziano J, Yu H, Fiehn O, Clarke OB, Frank J, Fan QR
Nature (2020) 584 pp. 304-309 [ Pubmed: 32581365 DOI: doi:10.1038/s41586-020-2452-0 ]
- [(2r)-3-[(z)-icos-11-enoyl]oxy-2-[(z)-octadec-9-enoyl]oxypropyl] 2-(trimethylazaniumyl)ethyl phosphate (814 Da, Ligand)
- Heterodimer human gaba(b) receptor composed of gaba(b1b) and gaba(b2) subunits. (193 kDa, Complex from Homo sapiens)
- Gamma-aminobutyric acid type b receptor subunit 1 (91 kDa, Protein from Homo sapiens)
- (2r)-3-{[(s)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-{[(9z)-octadec-9-enoyl]oxy}propyl (5z,8z,11z,14z)-icosa-5,8,11,14-tetraenoate (766 Da, Ligand)
- Gamma-aminobutyric acid type b receptor subunit 2 (93 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)