CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant, scFv16, and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ Pubmed: 39103320 DOI: doi:10.1038/s41467-024-50964-z ]
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Human drd2 in complex with heterotrimeric g protein goa (k46e), scfv16, and dopamine (120 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Escherichia coli)
- L-dopamine (153 Da, Ligand)
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ DOI: doi:10.1038/s41467-024-50964-z Pubmed: 39103320 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Human drd2 in complex with heterotrimeric g protein goa (k46e) and dopamine (120 kDa, Complex from Homo sapiens)
Structure of human GABA(B) receptor in an inactive state
Park J, Fu Z, Frangaj A, Liu J, Mosyak L, Shen T, Slavkovich VN, Ray KM, Taura J, Cao B, Geng Y, Zuo H, Kou Y, Grassucci R, Chen S, Liu Z, Lin X, Williams JP, Rice WJ, Eng ET, Huang RK, Soni RK, Kloss B, Yu Z, Javitch JA, Hendrickson WA, Slesinger PA, Quick M, Graziano J, Yu H, Fiehn O, Clarke OB, Frank J, Fan QR
Nature (2020) 584 pp. 304-309 [ DOI: 10.1038/s41586-020-2452-0 Pubmed: 32581365 ]
Image sets:
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6xeu
Q-score: 0.549
Mathiharan YK, Glaaser IW, Zhao Y, Robertson MJ, Skiniotis G, Slesinger PA
Cell Rep (2021) 36 pp. 109619- [ DOI: doi:10.1016/j.celrep.2021.109619 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- G protein-gated inwardly rectifying potassium channel (girk2) (Complex from Mus musculus)
- G protein-activated inward rectifier potassium channel 2 (39 kDa, Protein from Mus musculus)
- Potassium ion (39 Da, Ligand)
- Sodium ion (22 Da, Ligand)
Mathiharan YK, Glaaser IW, Zhao Y, Robertson MJ, Skiniotis G, Slesinger PA
Cell Rep (2021) 36 pp. 109619- [ DOI: doi:10.1016/j.celrep.2021.109619 ]
Mathiharan YK, Glaaser IW, Zhao Y, Robertson MJ, Skiniotis G, Slesinger PA
Cell Rep (2021) 36 pp. 109619- [ DOI: doi:10.1016/j.celrep.2021.109619 ]
Mathiharan YK, Glaaser IW, Zhao Y, Robertson MJ, Skiniotis G, Slesinger PA
Cell Rep (2021) 36 pp. 109619- [ DOI: doi:10.1016/j.celrep.2021.109619 ]
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6xev
Q-score: 0.52
Mathiharan YK, Glaaser IW, Zhao Y, Robertson MJ, Skiniotis G, Slesinger PA
Cell Rep (2021) 36 pp. 109619- [ DOI: doi:10.1016/j.celrep.2021.109619 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- G protein-activated inward rectifier potassium channel 2 (39 kDa, Protein from Mus musculus)
- Potassium ion (39 Da, Ligand)
- G protein-gated inwardly rectifying potassium channel (girk2) (Complex from Mus musculus)
CryoEM structure of apo G protein-gated inwardly rectifying potassium channel GIRK2
Mathiharan YK, Glaaser IW, Zhao Y, Robertson MJ, Skiniotis G, Slesinger PA
Cell Rep (2021) 36 pp. 109619- [ DOI: doi:10.1016/j.celrep.2021.109619 ]
Structure of human GABA(B) receptor in an inactive state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6wiv
Q-score: 0.486
Park J, Fu Z, Frangaj A, Liu J, Mosyak L, Shen T, Slavkovich VN, Ray KM, Taura J, Cao B, Geng Y, Zuo H, Kou Y, Grassucci R, Chen S, Liu Z, Lin X, Williams JP, Rice WJ, Eng ET, Huang RK, Soni RK, Kloss B, Yu Z, Javitch JA, Hendrickson WA, Slesinger PA, Quick M, Graziano J, Yu H, Fiehn O, Clarke OB, Frank J, Fan QR
Nature (2020) 584 pp. 304-309 [ Pubmed: 32581365 DOI: doi:10.1038/s41586-020-2452-0 ]
- (2r)-3-{[(s)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-{[(9z)-octadec-9-enoyl]oxy}propyl (5z,8z,11z,14z)-icosa-5,8,11,14-tetraenoate (766 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Heterodimer human gaba(b) receptor composed of gaba(b1b) and gaba(b2) subunits. (193 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid type b receptor subunit 2 (93 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid type b receptor subunit 1 (91 kDa, Protein from Homo sapiens)
- [(2r)-3-[(z)-icos-11-enoyl]oxy-2-[(z)-octadec-9-enoyl]oxypropyl] 2-(trimethylazaniumyl)ethyl phosphate (814 Da, Ligand)