Cryo-EM structure of BAF-Lamin A/C IgF-nucleosome complex (High mobility complex)
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 9j8m
Q-score: 0.345
Horikoshi N, Miyake R, Sogawa-Fujiwara C, Ogasawara M, Takizawa Y, Kurumizaka H
Nat Commun (2025) 16 pp. 1495-1495 [ Pubmed: 39929866 DOI: doi:10.1038/s41467-025-56823-9 ]
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (193-mer) (59 kDa, DNA from synthetic construct)
- Dna (193-mer) (59 kDa, DNA from synthetic construct)
- High mobility complex containing baf, lamin a/c igf, and nucleosome (270 kDa, Complex from Homo sapiens)
- Barrier-to-autointegration factor (10 kDa, Protein from Homo sapiens)
- Lamin-a/c (17 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
Cryo-EM structure of BAF-Lamin A/C IgF-nucleosome complex (Low mobility complex)
Resolution: 7.14 Å
EM Method: Single-particle
Fitted PDBs: 9j8n
Q-score: 0.164
Horikoshi N, Miyake R, Sogawa-Fujiwara C, Ogasawara M, Takizawa Y, Kurumizaka H
Nat Commun (2025) 16 pp. 1495-1495 [ Pubmed: 39929866 DOI: doi:10.1038/s41467-025-56823-9 ]
- Low mobility complex containing baf, lamin a/c igf, and nucleosome (620 kDa, Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (193-mer) (59 kDa, DNA from synthetic construct)
- Dna (193-mer) (59 kDa, DNA from synthetic construct)
- Barrier-to-autointegration factor (10 kDa, Protein from Homo sapiens)
- Lamin-a/c (17 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
Cryo-EM structure of BAF-Lamin A/C IgF-H1-nucleosome complex
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 9j8o
Q-score: 0.292
Horikoshi N, Miyake R, Sogawa-Fujiwara C, Ogasawara M, Takizawa Y, Kurumizaka H
Nat Commun (2025) 16 pp. 1495-1495 [ Pubmed: 39929866 DOI: doi:10.1038/s41467-025-56823-9 ]
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (193-mer) (59 kDa, DNA from synthetic construct)
- Histone h1.1 (22 kDa, Protein from Homo sapiens)
- Dna (193-mer) (59 kDa, DNA from synthetic construct)
- Barrier-to-autointegration factor (10 kDa, Protein from Homo sapiens)
- Lamin-a/c (17 kDa, Protein from Homo sapiens)
- Complex containing baf, lamin a/c igf, h1, and nucleosome (580 kDa, Complex from Homo sapiens)
- Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
Cryo-EM structure of human subnucleosome (closed form)
Resolution: 3.63 Å
EM Method: Single-particle
Fitted PDBs: 7x57
Q-score: 0.37
Nozawa K, Takizawa Y, Pierrakeas L, Sogawa-Fujiwara C, Saikusa K, Akashi S, Luk E, Kurumizaka H
PNAS (2022) 119 pp. e2206542119-e2206542119 [ DOI: doi:10.1073/pnas.2206542119 Pubmed: 36322721 ]
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Widom601 dna fw (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h3.1,histone h4 (Complex from Homo sapiens)
- Widom601 dna fw (145-mer),widom601 dna rw (145-mer) (Complex from synthetic construct)
- Widom601 dna rv (145-mer) (44 kDa, DNA from synthetic construct)
- Cryo-em structure of human subnucleosome (closed form) (Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of human subnucleosome (open form)
Resolution: 3.93 Å
EM Method: Single-particle
Fitted PDBs: 7x58
Q-score: 0.35
Nozawa K, Takizawa Y, Pierrakeas L, Sogawa-Fujiwara C, Saikusa K, Akashi S, Luk E, Kurumizaka H
PNAS (2022) 119 pp. e2206542119-e2206542119 [ DOI: doi:10.1073/pnas.2206542119 Pubmed: 36322721 ]
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Widom601 dna fw (145-mer),widom601 dna rv (145-mer) (Complex from synthetic construct)
- Widom601 dna fw (145-mer) (44 kDa, DNA from synthetic construct)
- Cryo-em structure of human subnucleosome (open form) (Complex)
- Histone h3.1,histone h4 (Complex from Homo sapiens)
- Widom601 dna rv (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of human subnucleosome (intermediate form)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7yoz
Q-score: 0.246
Nozawa K, Takizawa Y, Pierrakeas L, Sogawa-Fujiwara C, Saikusa K, Akashi S, Luk E, Kurumizaka H
PNAS (2022) 119 pp. e2206542119-e2206542119 [ DOI: doi:10.1073/pnas.2206542119 Pubmed: 36322721 ]
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Widom601 dna fw (145-mer),widom601 dna rv (145-mer) (Complex from synthetic construct)
- Widom601 dna fw (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h3.1,histone h4 (Complex from Homo sapiens)
- Widom601 dna rv (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Cryo-em structure of human subnucleosome (intermediate form) (Complex)