Spinelli S, Tremblay D, Moineau S, Cambillau C, Goulet A
Viruses (2020) 12 [ Pubmed: 32796652 DOI: doi:10.3390/v12080878 ]
Resolution: 42.2 Å
EM Method: Single-particle
Fitted PDBs: 6zig
Q-score: 0.028
Spinelli S, Tremblay D, Moineau S, Cambillau C, Goulet A
Viruses (2020) 12 [ Pubmed: 32796652 DOI: doi:10.3390/v12080878 ]
- Baseplate protein gp16 (42 kDa, Protein from Lactococcus phage p2)
- Receptor binding protein (28 kDa, Protein from Lactococcus phage p2)
- Distal tail protein (34 kDa, Protein from Lactococcus phage p2)
- Lactococcus virus p2 (Virus from Lactococcus virus P2)
Negative staining 3D reconstruction of p2 virion baseplate in activated conformation
Resolution: 28.7 Å
EM Method: Single-particle
Fitted PDBs: 6zih
Q-score: 0.033
Spinelli S, Tremblay D, Moineau S, Cambillau C, Goulet A
Viruses (2020) 12 [ Pubmed: 32796652 DOI: doi:10.3390/v12080878 ]
- Baseplate protein gp16 (42 kDa, Protein from Lactococcus phage p2)
- Receptor binding protein (28 kDa, Protein from Lactococcus phage p2)
- Distal tail protein (34 kDa, Protein from Lactococcus phage p2)
- Lactococcus virus p2 (Virus from Lactococcus virus P2)
Structure of the phage STP1 host adhesion device (baseplate) by negative staining
Lavelle K, Goulet A, McDonnell B, Spinelli S, van Sinderen D, Mahony J, Cambillau C
Microb Biotechnol (2020) 13 pp. 1765-1779 [ DOI: doi:10.1111/1751-7915.13593 Pubmed: 32525270 ]
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rj9
Q-score: 0.542
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ Pubmed: 31604602 DOI: doi:10.1016/j.molcel.2019.09.012 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Acriia6 (Complex from Streptococcus phage D1811)
- Ntpam (Complex from Streptococcus virus 2972)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 monomeric assembly. (Complex)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Sgrna (Complex from Streptococcus thermophilus)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rja
Q-score: 0.508
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ Pubmed: 31604602 DOI: doi:10.1016/j.molcel.2019.09.012 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Acriia6 (Complex from Streptococcus phage D1811)
- Tdna20 (Complex from Streptococcus virus 2972)
- Rna (78-mer) (Complex from Streptococcus thermophilus)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 dimeric assembly. (Complex)
- Rna (78-mer) (37 kDa, RNA from Streptococcus thermophilus)
Cryo-EM structure of St1Cas9-sgRNA-tDNA59-ntPAM complex.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6rjd
Q-score: 0.5
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ Pubmed: 31604602 DOI: doi:10.1016/j.molcel.2019.09.012 ]
- Sgrna (78-mer) (37 kDa, RNA from Streptococcus thermophilus DGCC 7710)
- Tdna59 (18 kDa, DNA from Streptococcus phage D1811)
- Streptococcus thermophilus 1 cas9 (Complex from Streptococcus thermophilus DGCC 7710)
- Cryo-em structure of st1cas9-sgrna-tdna59-ntpam complex. (Complex)
- Tdna59 (Complex from Streptococcus phage D1811)
- Streptococcus thermophilus 1 cas9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Ntpam (Complex from Streptococcus phage 2972)
- Ntpam (7 kDa, DNA from Streptococcus Phage 2972)
- Sgrna (78-mer) (Complex from Streptococcus thermophilus)
Cryo-EM structure of St1Cas9-sgRNA-AcrIIA6-tDNA59-ntPAM complex.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rjg
Q-score: 0.522
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ Pubmed: 31604602 DOI: doi:10.1016/j.molcel.2019.09.012 ]
- Cryo-em structure of st1cas9-sgrna-acriia6-tdna59-ntpam complex. (Complex)
- Tdna59 (Complex from Streptococcus virus 2972)
- Cas 9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Acriia6 (Complex from Streptococcus phage D1811)
- Cas 9 (Complex from Streptococcus thermophilus DGCC 7710)
- Ntpam (Complex from Streptococcus virus 2972)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Sgrna (Complex from Streptococcus thermophilus)
- Tdna59 (18 kDa, DNA from Streptococcus virus 2972)
- Ntpam (7 kDa, DNA from Streptococcus virus 2972)
Structure of the baseplate of Siphophage J-1
Dieterle ME, Spinelli S, Sadovskaya I, Piuri M, Cambillau C
Mol Microbiol (2017) 104 pp. 608-620 [ DOI: doi:10.1111/mmi.13649 Pubmed: 28196397 ]
Negative stain electron microscopy structure of TssA from EAEC type VI secretion system
Zoued A, Durand E, Brunet YR, Spinelli S, Douzi B, Guzzi M, Flaugnatti N, Legrand P, Journet L, Fronzes R, Mignot T, Cambillau C, Cascales E
Nature (2016) 531 pp. 59-63 [ DOI: doi:10.1038/nature17182 Pubmed: 26909579 ]
- Tssa dodecamer (952 MDa, Sample)
- Tssa (74 kDa, Protein from Escherichia coli 042)