Cryo electron microscopy of inactive hyaluronan synthase with UDP-GlcNAc
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: 10.1038/s41586-022-04534-2 ]
Image sets:
Cryo electron microscopy of wild-type hyaluronan synthase with UDP
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: 10.1038/s41586-022-04534-2 ]
Image sets:
Chlorella virus hyaluronan synthase
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7sp6
Q-score: 0.454
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: doi:10.1038/s41586-022-04534-2 ]
- Nanobody 872 (14 kDa, Protein from Lama glama)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Hyaluronan synthase in nanodiscs in complex with two camelid nanobodies. (95 kDa, Complex from Paramecium bursaria Chlorella virus CZ-2)
- Manganese (ii) ion (54 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
Chlorella virus hyaluronan synthase inhibited by UDP
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7sp7
Q-score: 0.475
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: doi:10.1038/s41586-022-04534-2 ]
- Nanobody 872 (14 kDa, Protein from Lama glama)
- Uridine-5'-diphosphate (404 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Hyaluronan synthase in nanodiscs in complex with two camelid nanobodies (95 kDa, Complex from Paramecium bursaria Chlorella virus CZ-2)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Manganese (ii) ion (54 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
Chlorella virus Hyaluronan Synthase bound to UDP-GlcNAc
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sp8
Q-score: 0.536
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: doi:10.1038/s41586-022-04534-2 ]
- Nanobody 872 (14 kDa, Protein from Lama glama)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Uridine-diphosphate-n-acetylglucosamine (607 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Hyaluronan synthase in nanodiscs in complex with two camelid nanobodies. (95 kDa, Complex from Paramecium bursaria Chlorella virus CZ-2)
- Manganese (ii) ion (54 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
Chlorella virus Hyaluronan Synthase in the GlcNAc-primed channel-closed state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7sp9
Q-score: 0.486
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: doi:10.1038/s41586-022-04534-2 ]
- Nanobody 872 (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Hyaluronan synthase in nanodiscs in complex with two camelid nanobodies. (95 kDa, Complex from Paramecium bursaria Chlorella virus CZ-2)
- Cholesterol hemisuccinate (486 Da, Ligand)
Chlorella virus Hyaluronan Synthase in the GlcNAc-primed, channel-open state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7spa
Q-score: 0.472
Maloney FP, Kuklewicz J, Corey RA, Bi Y, Ho R, Mateusiak L, Pardon E, Steyaert J, Stansfeld PJ, Zimmer J
Nature (2022) 604 pp. 195-201 [ Pubmed: 35355017 DOI: doi:10.1038/s41586-022-04534-2 ]
- Nanobody 872 (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hyaluronan synthase (65 kDa, Protein from Paramecium bursaria Chlorella virus CZ-2)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nanobody 881 (15 kDa, Protein from Lama glama)
- Hyaluronan synthase in nanodiscs in complex with two camelid nanobodies. (95 kDa, Complex from Paramecium bursaria Chlorella virus CZ-2)
- Cholesterol hemisuccinate (486 Da, Ligand)