Single particle cryo-EM of KdpFABC WT (KdpB-Ser162-P) under turnover condition
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C
eLife (2022) 11 [ DOI: 10.7554/elife.80988 Pubmed: 36255052 ]
Image sets:
- Unaligned movie frames of the KdpFABC WT (KdpB-Ser162-P) under turnover conditions in DDM (Dataset 3)
- Unaligned movie frames of the KdpFABC WT (KdpB-Ser162-P) under turnover conditions in DDM (Dataset 4)
- Unaligned movie frames of the KdpFABC WT (KdpB-Ser162-P) under turnover conditions in DDM (Dataset 5)
- Unaligned movie frames of the KdpFABC WT (KdpB-Ser162-P) under turnover conditions in DDM (Dataset 2)
- Unaligned movie frames of the KdpFABC WT (KdpB-Ser162-P) under turnover conditions in DDM (Dataset 1)
Single particle cryo-EM of KdpFAB(Ser162-P, D307N)C in apo condition
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C
eLife (2022) 11 [ DOI: 10.7554/elife.80988 Pubmed: 36255052 ]
Image sets:
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 8)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 4)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 6)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 15)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 2)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 13)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 3)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 5)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 1)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 11)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 7)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 9)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 14)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 12)
- Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 10)
Single particle cryo-EM of KdpFABC WT (KdpB-Ser162-P) in presence of orthovanadate
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C
eLife (2022) 11 [ DOI: 10.7554/elife.80988 Pubmed: 36255052 ]
Image sets:
Single particle cryo-EM of KdpFAB(S162A)C under turnover condition
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C
eLife (2022) 11 [ DOI: 10.7554/elife.80988 Pubmed: 36255052 ]
Image sets:
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (Protein from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7zrd
Q-score: 0.504
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Vanadate ion (114 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1-P tight conformation, under turnover conditions
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zre
Q-score: 0.487
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1_ATPearly conformation, under turnover conditions
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zrg
Q-score: 0.465
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zrh
Q-score: 0.485
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zri
Q-score: 0.457
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Kdpfabc (157 kDa, Complex from Escherichia coli)