Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8smv
Q-score: 0.572
Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A
Nat Struct Mol Biol (2024) 31 pp. 667-677 [ Pubmed: 38326651 DOI: doi:10.1038/s41594-024-01223-8 ]
- G-protein coupled receptor 161 (59 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 161, guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Gpr161 gs heterotrimeric complex with nb35 (Complex)
- Cholesterol (386 Da, Ligand)
Heterodimeric ABC exporter TmrAB in inward-facing narrow conformation under turnover conditions
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6raf
Q-score: 0.407
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Tmrab in inward-facing narrow conformation (150 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Anti-vesicular stomatitis virus n vhh (Complex from Vicugna pacos)
- Multidrug resistance abc transporter atp-binding and permease protein (Complex from Thermus thermophilus HB27)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Anti-vesicular stomatitis virus n vhh (14 kDa, Protein from Vicugna pacos)
- Multidrug resistance abc transporter atp-binding and permease protein (Complex from Thermus thermophilus)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus HB27)
Heterodimeric ABC exporter TmrAB in inward-facing wide conformation under turnover conditions
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6rag
Q-score: 0.356
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Tmrab in inward-facing wide conformation (150 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Nanobody (Complex from Vicugna pacos)
- Tmrab (Complex from Thermus thermophilus)
Heterodimeric ABC exporter TmrAB in ATP-bound outward-facing open conformation
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6rah
Q-score: 0.534
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Tmrab eq mutant in atp bound outward-facing open conformation (150 kDa, Complex)
- Water (18 Da, Ligand)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Tmrab eq mutant in atp bound outward-facing open conformation (Complex from Thermus thermophilus)
- Tmrab eq mutant in atp bound outward-facing open conformation (Complex from Vicugna pacos)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus)
Heterodimeric ABC exporter TmrAB in ATP-bound outward-facing occluded conformation
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6rai
Q-score: 0.539
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Tmrab eq mutant in atp bound outward-occluded conformation (150 kDa, Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Nanobody (Complex from Vicugna pacos)
- Tmrab (Complex from Thermus thermophilus)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus)
Heterodimeric ABC exporter TmrAB in vanadate trapped outward-facing open conformation
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6raj
Q-score: 0.481
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Multidrug resistance abc transporter atp-binding and permease protein (Complex from Thermus thermophilus HB27)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Tmrab in vanadate trapped outward facing open conformation (150 kDa, Complex)
- Multidrug resistance abc transporter atp-binding and permease protein (Complex from Thermus thermophilus)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus HB27)
- Adp orthovanadate (544 Da, Ligand)
- Nanobody nb9f10 (Complex from Vicugna pacos)
Heterodimeric ABC exporter TmrAB in vanadate trapped outward-facing occluded conformation
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6rak
Q-score: 0.531
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus)
- Tmrab in vanadate trapped of occluded conformation (150 kDa, Complex)
- Tmrab in vanadate trapped of occluded conformation (Complex from Vicugna pacos)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Tmrab in vanadate trapped of occluded conformation (Complex from Thermus thermophilus)
- Adp orthovanadate (544 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6ral
Q-score: 0.488
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus)
- Tmrab in asymmetric unlocked return conformation under turnover conditions (150 kDa, Complex)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Multidrug resistance abc transporter atp-binding and permease protein (Complex from Thermus thermophilus)
- Nanobody nb9f10 (Complex from Vicugna pacos)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ram
Q-score: 0.378
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Bruchert S, Kuhn BT, Geertsma ER, Hummer G, Tampe R, Moeller A
Nature (2019) 571 pp. 580-583 [ Pubmed: 31316210 DOI: doi:10.1038/s41586-019-1391-0 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (64 kDa, Protein from Thermus thermophilus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Multidrug resistance abc transporter atp-binding and permease protein (70 kDa, Protein from Thermus thermophilus)
- Nanobody nb9f10 (14 kDa, Protein from Vicugna pacos)
- Nanobody (Complex from Vicugna pacos)
- Tmrab (Complex from Thermus thermophilus)
- Tmrab under turnover conditions in asymmetric unlocked return conformation with wider opened intracellular gate (150 kDa, Complex)