cryo-EM of human Gastric inhibitory polypeptide receptor GIPR bound to GIP
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7ra3
Q-score: 0.436
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ DOI: doi:10.1073/pnas.2116506119 Pubmed: 35333651 ]
- Gastric inhibitory polypeptide receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-3,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Gastric inhibitory polypeptide gip bound to gip receptor gipr (Complex from Homo sapiens)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Gastric inhibitory polypeptide (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em of human gastric inhibitory polypeptide receptor gipr bound to gip, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
cryo-EM structure of human Gastric inhibitory polypeptide receptor GIPR bound to tirzepatide
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7rbt
Q-score: 0.479
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ DOI: doi:10.1073/pnas.2116506119 Pubmed: 35333651 ]
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Tirzepatide bound to gip receptor gipr (Complex from Homo sapiens)
- Gastric inhibitory polypeptide receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- 2-fluoro-4-[(1r)-6-methoxy-1-methyl-2-{(1s)-1-[4-(propan-2-yl)phenyl]ethyl}-1,2,3,4-tetrahydroisoquinolin-5-yl]-6-[(2-methylpropyl)amino]phenol (504 Da, Ligand)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- Cryo-em of human gastric inhibitory polypeptide receptor gipr bound to tirzepatide, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R in apo form
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7rg9
Q-score: 0.476
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ DOI: doi:10.1073/pnas.2116506119 Pubmed: 35333651 ]
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor in apo form (Complex from Homo sapiens)
- Cryo-em of apo form human glucagon-like peptide 1 receptor glp-1r, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R bound to tirzepatide
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7rgp
Q-score: 0.484
Sun B, Willard FS, Feng D, Alsina-Fernandez J, Chen Q, Vieth M, Ho JD, Showalter AD, Stutsman C, Ding L, Suter TM, Dunbar JD, Carpenter JW, Mohammed FA, Aihara E, Brown RA, Bueno AB, Emmerson PJ, Moyers JS, Kobilka TS, Coghlan MP, Kobilka BK, Sloop KW
PNAS (2022) 119 pp. e2116506119-e2116506119 [ DOI: doi:10.1073/pnas.2116506119 Pubmed: 35333651 ]
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor bound to tirzepatide (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-3,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Single-chain variable fragment 16 (31 kDa, Protein from Mus musculus)
- Tirzepatide (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em of human glucagon-like peptide 1 receptor glp-1r bound to tirzepatide, trimeric g protein complex and stabilizing antibodies (150 kDa, Complex)
cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6xox
Q-score: 0.482
Kawai T, Sun B, Yoshino H, Feng D, Suzuki Y, Fukazawa M, Nagao S, Wainscott DB, Showalter AD, Droz BA, Kobilka TS, Coghlan MP, Willard FS, Kawabe Y, Kobilka BK, Sloop KW
PNAS (2020) 117 pp. 29959-29967 [ DOI: doi:10.1073/pnas.2014879117 Pubmed: 33177239 ]
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Cryo-em structure of the glucagon-like peptide-1 receptor in complex with g protein and a non-peptide agonist ly3502970 (150 kDa, Complex)
- Glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Alpha subunit of gs with n-terminus swapped with equivalent residues in gi,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (43 kDa, Protein from Homo sapiens)
- Single-chain variable fragment 16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Trimeric stimulatory g protein (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- 3-[(1s,2s)-1-(5-[(4s)-2,2-dimethyloxan-4-yl]-2-{(4s)-2-(4-fluoro-3,5-dimethylphenyl)-3-[3-(4-fluoro-1-methyl-1h-indazol-5-yl)-2-oxo-2,3-dihydro-1h-imidazol-1-yl]-4-methyl-2,4,6,7-tetrahydro-5h-pyrazolo[4,3-c]pyridine-5-carbonyl}-1h-indol-1-yl)-2-methylcyclopropyl]-1,2,4-oxadiazol-5(4h)-one (882 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Mus musculus)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6vcb
Q-score: 0.452
Bueno AB, Sun B, Willard FS, Feng D, Ho JD, Wainscott DB, Showalter AD, Vieth M, Chen Q, Stutsman C, Chau B, Ficorilli J, Agejas FJ, Cumming GR, Jimenez A, Rojo I, Kobilka TS, Kobilka BK, Sloop KW
Nat Chem Biol (2020) 16 pp. 1105-1110 [ DOI: doi:10.1038/s41589-020-0589-7 Pubmed: 32690941 ]
- Glucagon-like peptide 1 receptor (51 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- 1-[(1r)-1-(2,6-dichloro-3-methoxyphenyl)ethyl]-6-{2-[(2r)-piperidin-2-yl]phenyl}-1h-benzimidazole (480 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Cryo-em structure of the glucagon-like peptide-1 receptor in complex with g protein, glp-1 peptide and a positive allosteric modulator (150 kDa, Complex)
- Glucagon-like peptide 1 receptor, glucagon-like peptide 1 (Complex from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)
- Glucagon-like peptide 1 (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
Apo form metabotropic glutamate receptor 5 with Nanobody 43
Koehl A, Hu H, Feng D, Sun B, Zhang Y, Robertson MJ, Chu M, Kobilka TS, Laermans T, Steyaert J, Tarrasch J, Dutta S, Fonseca R, Weis WI, Mathiesen JM, Skiniotis G, Kobilka BK
Nature (2019) 566 pp. 79-84 [ DOI: doi:10.1038/s41586-019-0881-4 Pubmed: 30675062 ]
- Nanobody 43 (Protein from Lama glama)
- Metabotropic glutamate receptor 5 - nb43 complex (220 kDa, Complex from Homo sapiens)
- Metabotropic glutamate receptor 5 (Protein from Homo sapiens)
Metabotropic Glutamate Receptor 5 bound to L-quisqualate and Nb43
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6n51
Q-score: 0.341
Koehl A, Hu H, Feng D, Sun B, Zhang Y, Robertson MJ, Chu M, Kobilka TS, Laermans T, Steyaert J, Tarrasch J, Dutta S, Fonseca R, Weis WI, Mathiesen JM, Skiniotis G, Kobilka BK
Nature (2019) 566 pp. 79-84 [ DOI: doi:10.1038/s41586-019-0881-4 Pubmed: 30675062 ]
- Nanobody 43 (13 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Metabotropic glutamate receptor 5 (90 kDa, Protein from Homo sapiens)
- Complex between metabotropic glutamate receptor 5 bound to l-quisqualate and nb43 (220 kDa, Complex from Homo sapiens)
- (s)-2-amino-3-(3,5-dioxo-[1,2,4]oxadiazolidin-2-yl)-propionic acid (189 Da, Ligand)
Metabotropic Glutamate Receptor 5 Apo Form
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6n52
Q-score: 0.312
Koehl A, Hu H, Feng D, Sun B, Zhang Y, Robertson MJ, Chu M, Kobilka TS, Laermans T, Steyaert J, Tarrasch J, Dutta S, Fonseca R, Weis WI, Mathiesen JM, Skiniotis G, Kobilka BK
Nature (2019) 566 pp. 79-84 [ DOI: doi:10.1038/s41586-019-0881-4 Pubmed: 30675062 ]
- Metabotropic glutamate receptor 5 (97 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Metabotropic glutamate receptor 5 in nanodiscs (200 kDa, Complex from Homo sapiens)
Cryo-EM structure of the activated Glucagon-like peptide-1 receptor in complex with G protein
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5vai
Q-score: 0.297
Zhang Y, Sun B, Feng D, Hu H, Chu M, Qu Q, Tarrasch JT, Li S, Sun Kobilka T, Kobilka BK, Skiniotis G
Nature (2017) 546 pp. 248-253 [ DOI: doi:10.1038/nature22394 Pubmed: 28538729 ]
- The activated glucagon-like peptide-1 receptor in complex with the heterotrimeric gs protein (150 kDa, Complex from Oryctolagus cuniculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Uncharacterized protein (52 kDa, Protein from Oryctolagus cuniculus)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Glucagon-like peptide 1 (3 kDa, Protein from Homo sapiens)