Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.5 angstrom
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7xjg
Q-score: 0.515
Wang Y, Guan Z, Wang C, Nie Y, Chen Y, Qian Z, Cui Y, Xu H, Wang Q, Zhao F, Zhang D, Tao P, Sun M, Yin P, Jin S, Wu S, Zou T
Nat Microbiol (2022) 7 pp. 1480-1489 [ DOI: doi:10.1038/s41564-022-01197-7 Pubmed: 35982312 ]
- Magnesium ion (24 Da, Ligand)
- Rna (81-mer) (25 kDa, RNA from Escherichia coli)
- Retron st85 family effector protein (35 kDa, Protein from Escherichia coli)
- Rna (14-mer) (4 kDa, RNA from Escherichia coli)
- Retron-ec86 (rt-msdna-rna) (Complex from Escherichia coli)
- Rna-directed dna polymerase from retron ec86 (37 kDa, Protein from Escherichia coli)
- Dna (105-mer) (32 kDa, DNA from Escherichia coli)
Cryo-EM structure of Dot1L and H2BK34ub-H3K79Nle nucleosome 2:1 complex
Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 7xct
Q-score: 0.514
Ai H, Sun M, Liu A, Sun Z, Liu T, Cao L, Liang L, Qu Q, Li Z, Deng Z, Tong Z, Chu G, Tian X, Deng H, Zhao S, Li JB, Lou Z, Liu L
Nat Chem Biol (2022) 18 pp. 972-980 [ Pubmed: 35739357 DOI: doi:10.1038/s41589-022-01067-7 ]
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Complex of dot1l(1-416) and h2bk34ub 2:1 nucleosome (Complex from Homo sapiens)
- Histone h4 (10 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- S-adenosylmethionine (398 Da, Ligand)
- Histone h2a (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Histone domain-containing protein (11 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (37 kDa, Protein from Homo sapiens)
Tetrameric human mitochondrial Hsp90 (TRAP1) in the presence of AMP-PNP
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7klu
Q-score: 0.451
Liu YX, Agard DA, Elnatan D, Sun M, Larson AG
To Be Published
- Tetrameric human mitochondrial hsp90 (trap1) in the presence of amp-pnp (360 kDa, Complex from Homo sapiens)
- Heat shock protein 75 kda, mitochondrial, spycatcher (87 kDa, Protein from synthetic construct)
- Potassium ion (39 Da, Ligand)
- Heat shock protein 75 kda, mitochondrial, spytag, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (chimera) (93 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
SARS-CoV-2 Spike bound to Nb6 in closed conformation
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7kkk
Q-score: 0.463
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ Pubmed: 33154106 DOI: doi:10.1126/science.abe3255 ]
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Synthetic nanobody nb6 (Complex from synthetic construct)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Complex of spike s2p glycoprotein with synthetic nanobody nb6 (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody nb6 (12 kDa, Protein from synthetic construct)
SARS-CoV-2 Spike bound to mNb6 in closed conformation
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7kkl
Q-score: 0.514
Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbolle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, Garcia-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A
Science (2020) 370 pp. 1473-1479 [ DOI: doi:10.1126/science.abe3255 Pubmed: 33154106 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Synthetic nanobody mnb6 (13 kDa, Protein from synthetic construct)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Complex of spike s2p glycoprotein with synthetic nanobody mnb6 (Complex)
- Synthetic nanobody mnb6 (Complex from synthetic construct)
- Spike s2p glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)