Sun M, Azumaya CM, Tse E, Bulkley DP, Harrington MB, Gilbert G, Frost A, Southworth D, Verba KA, Cheng Y, Agard DA
J Struct Biol (2021) 213 [ Pubmed: 33984504 DOI: 10.1016/j.jsb.2021.107745 ]
Image sets:
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in CDS mode from Gatan K3 at lower magnification
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3 at lower magnification
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in standard mode from Gatan K3 at lower magnification
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3 at lower magnification
- apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3
Tetrameric human mitochondrial Hsp90 (TRAP1) in the presence of AMP-PNP
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7klu
Q-score: 0.451
Liu YX, Agard DA, Elnatan D, Sun M, Larson AG
To Be Published
- Tetrameric human mitochondrial hsp90 (trap1) in the presence of amp-pnp (360 kDa, Complex from Homo sapiens)
- Heat shock protein 75 kda, mitochondrial, spycatcher (87 kDa, Protein from synthetic construct)
- Potassium ion (39 Da, Ligand)
- Heat shock protein 75 kda, mitochondrial, spytag, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (chimera) (93 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7klv
Q-score: 0.528
Liu YX, Agard DA, Elnatan D, Sun M, Larson AG
To Be Published
- Heat shock protein 75 kda, mitochondrial, spycatcher (87 kDa, Protein from synthetic construct)
- Full-length human mitochondrial hsp90 (trap1) spycatcher/spytag-sdhb heterodimer in the presence of amp-pnp (180 kDa, Complex from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Heat shock protein 75 kda, mitochondrial, spytag, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (chimera) (93 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Full-length human mitochondrial Hsp90 (TRAP1) with AMP-PNP
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 7kck
Q-score: 0.477
Liu YX, Agard DA, Elnatan D, Sun M, Myasnikov AG
To Be Published
- Magnesium ion (24 Da, Ligand)
- Heat shock protein 75 kda, mitochondrial (74 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Human trap1 dimer with amp-pnp (147 kDa, Complex from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Full-length human mitochondrial Hsp90 (TRAP1) in complex with SdhB in the presence of AMP-PNP
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 7kcl
Q-score: 0.506
Liu YX, Agard DA, Elnatan D, Sun M, Myasnikov AG
To Be Published
- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (15 kDa, Protein from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Human trap1 in complex with sdhb in the presence of amp-pnp (162 kDa, Complex from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Heat shock protein 75 kda, mitochondrial (74 kDa, Protein from Homo sapiens)
Liu YX, Agard DA, Elnatan D, Sun M, Myasnikov AG
To Be Published
- Trap1 (Protein from Homo sapiens)
- Human trap1 in complex with sdhb in the presence of amp-pnp (162 kDa, Complex from Homo sapiens)
- Sdhb (Protein from Homo sapiens)
Human TRAP1 binding with SdhB folding intermediate state in the presence of AMP-PNP
Liu YX, Agard DA, Elnatan D, Sun M, Myasnikov AG
To Be Published
- Trap1 (Protein from Homo sapiens)
- Human trap1 in complex with sdhb in the presence of amp-pnp (162 kDa, Complex from Homo sapiens)
- Sdhb (Protein from Homo sapiens)
Full-length human mitochondrial Hsp90 (TRAP1) in complex with SdhB in the presence of AMP-PNP
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 7kcm
Q-score: 0.459
Liu YX, Agard DA, Elnatan D, Sun M, Myasnikov AG
To Be Published
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Human trap1 in complex with sdhb in the presence of amp-pnp (162 kDa, Complex from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (15 kDa, Protein from Homo sapiens)
- Heat shock protein 75 kda, mitochondrial (74 kDa, Protein from Homo sapiens)
Apo full-length Hsc82 in complex with Aha1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6xlb
Q-score: 0.343
Liu YX, Sun M, Myasnikov AG, Elnatan D, Agard DA
To Be Published
- Hsc82 in complex with aha1 ctd (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Atp-dependent molecular chaperone hsc82 (81 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hsp90 co-chaperone aha1 (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))