BAP1/ASXL1 bound to the H2AK119Ub Nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8svf
Q-score: 0.383
Thomas JF, Valencia-Sanchez MI, Tamburri S, Gloor SL, Rustichelli S, Godinez-Lopez V, De Ioannes P, Lee R, Abini-Agbomson S, Gretarsson K, Burg JM, Hickman AR, Sun L, Gopinath S, Taylor HF, Sun ZW, Ezell RJ, Vaidya A, Meiners MJ, Cheek MA, Rice WJ, Svetlov V, Nudler E, Lu C, Keogh MC, Pasini D, Armache KJ
Sci Adv (2023) 9 pp. eadg9832-eadg9832 [ Pubmed: 37556531 DOI: doi:10.1126/sciadv.adg9832 ]
- Dna/rna (187-mer) (58 kDa, Macromolecule from synthetic construct)
- Ubiquitin carboxyl-terminal hydrolase bap1 (80 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Dna/rna (327-mer) (102 kDa, Macromolecule from synthetic construct)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Bap1/asxl1 bound to the h2ak119ub nucleosome (Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Polycomb group protein asxl1 (165 kDa, Protein from Homo sapiens)
Map focused on acidic patch BAP1/ASXL1 bound to the H2AK119Ub Nucleosome
Thomas JF, Valencia-Sanchez MI, Tamburri S, Gloor SL, Rustichelli S, Godinez-Lopez V, De Ioannes P, Lee R, Abini-Agbomson S, Gretarsson K, Burg JM, Hickman AR, Sun L, Gopinath S, Taylor HF, Sun ZW, Ezell RJ, Vaidya A, Meiners MJ, Cheek MA, Rice WJ, Svetlov V, Nudler E, Lu C, Keogh MC, Pasini D, Armache KJ
Sci Adv (2023) 9 pp. eadg9832-eadg9832 [ Pubmed: 37556531 DOI: doi:10.1126/sciadv.adg9832 ]
Overall map of BAP1/ASXL1 bound to the H2AK119Ub Nucleosome
Thomas JF, Valencia-Sanchez MI, Tamburri S, Gloor SL, Rustichelli S, Godinez-Lopez V, De Ioannes P, Lee R, Abini-Agbomson S, Gretarsson K, Burg JM, Hickman AR, Sun L, Gopinath S, Taylor HF, Sun ZW, Ezell RJ, Vaidya A, Meiners MJ, Cheek MA, Rice WJ, Svetlov V, Nudler E, Lu C, Keogh MC, Pasini D, Armache KJ
Sci Adv (2023) 9 pp. eadg9832-eadg9832 [ Pubmed: 37556531 DOI: doi:10.1126/sciadv.adg9832 ]
CryoEM Structure of E. coli Transcription-Coupled Ribonucleotide Excision Repair (TC-RER) complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7uwe
Q-score: 0.413
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ Pubmed: 37196657 DOI: doi:10.1016/j.cell.2023.04.029 ]
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Elongation complex(ec)-rnaseh2 complex (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7uwh
Q-score: 0.458
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ Pubmed: 37196657 DOI: doi:10.1016/j.cell.2023.04.029 ]
- Dna/rna (59-mer) (18 kDa, Macromolecule from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna (59-mer) (18 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
- Elongation complex(ec)-rnaseh2 complex bound to ribonucleotide substrate (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Single particle cryo-EM of the human CST•Polα/Primase (POLA1 FL) complex in a recruitment state
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ DOI: 10.1038/s41594-022-00766-y Pubmed: 35578024 ]
Image sets:
Single particle cryo-EM of the human CST•Polα/Primase (POLA1ΔN) complex in a recruitment state
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ DOI: 10.1038/s41594-022-00766-y Pubmed: 35578024 ]
Image sets:
Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7u5c
Q-score: 0.33
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ Pubmed: 35578024 DOI: doi:10.1038/s41594-022-00766-y ]
- Dna polymerase alpha subunit b (66 kDa, Protein from Homo sapiens)
- Dna primase large subunit (59 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase small subunit (49 kDa, Protein from Homo sapiens)
- Cst complex subunit ten1 (13 kDa, Protein from Homo sapiens)
- Cst complex subunit stn1 (42 kDa, Protein from Homo sapiens)
- Human cst bound to dna polymerase alpha(pola1 delta n)-primase in a recruitment state (500 kDa, Complex)
- Canonical telomeric dna sequence (5 kDa, DNA from Homo sapiens)
- Cst complex subunit ctc1 (135 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Homo sapiens)
Human CST bound to full-length DNA polymerase alpha-primase in a recruitment state
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ Pubmed: 35578024 DOI: doi:10.1038/s41594-022-00766-y ]
- Dna polymerase alpha b subunit pola2 (Protein from Homo sapiens)
- Dna primase large subunit prim2 (Protein from Homo sapiens)
- Dna primase catalytic subunit (Protein from Homo sapiens)
- Cst complex subunit ctc1 (Protein from Homo sapiens)
- Telomeric g-strand ssdna (3 repeats) (DNA from Homo sapiens)
- Cst complex subunit ten1 (Protein from Homo sapiens)
- Dna polymerase alpha catalytic subunit pola1 (Protein from Homo sapiens)
- Cst complex subunit stn1 (Protein from Homo sapiens)
- Cst bound to full-length dna polymerase alpha-primase and ssdna (540 kDa, Complex)
Cryo-EM structure of PRC2:EZH1-AEBP2-JARID2
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7kso
Q-score: 0.379
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ DOI: doi:10.1038/s41467-020-20775-z Pubmed: 33514705 ]
- Prc2:ezh1-aebp2-jarid2 (330 kDa, Complex from Homo sapiens)
- Histone-lysine n-methyltransferase ezh1 (85 kDa, Protein from Homo sapiens)
- Protein jumonji (138 kDa, Protein from Homo sapiens)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- Polycomb protein eed (50 kDa, Protein from Homo sapiens)
- Histone-binding protein rbbp4 (47 kDa, Protein from Homo sapiens)
- Zinc finger protein aebp2 (33 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)