Cryo-EM map of Hrd1/Hrd3 monomer
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6vjy
Q-score: 0.334
Wu X, Siggel M, Ovchinnikov S, Mi W, Svetlov V, Nudler E, Liao M, Hummer G, Rapoport TA
Science (2020) 368 [ DOI: doi:10.1126/science.aaz2449 Pubmed: 32327568 ]
CryoEM map of Hrd1-Usa1/Der1/Hrd3 complex of the expected topology
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6vjz
Q-score: 0.334
Wu X, Siggel M, Ovchinnikov S, Mi W, Svetlov V, Nudler E, Liao M, Hummer G, Rapoport TA
Science (2020) 368 [ DOI: doi:10.1126/science.aaz2449 Pubmed: 32327568 ]
- Erad-associated e3 ubiquitin-protein ligase component hrd3 (88 kDa, Protein from Saccharomyces cerevisiae)
- Degradation in the endoplasmic reticulum protein 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Erad-associated e3 ubiquitin-protein ligase hrd1 (55 kDa, Protein from Saccharomyces cerevisiae)
- Complex of hrd1-usa1/der1/hrd3 in the expected topology (Complex from Saccharomyces cerevisiae)
- U1 snp1-associating protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
CryoEM map of Hrd1-Usa1/Der1/Hrd3 of the flipped topology
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6vk0
Q-score: 0.369
Wu X, Siggel M, Ovchinnikov S, Mi W, Svetlov V, Nudler E, Liao M, Hummer G, Rapoport TA
Science (2020) 368 [ Pubmed: 32327568 DOI: doi:10.1126/science.aaz2449 ]
- Complex of hrd1-usa1/der1/hrd3 of the flipped topology (Complex from Saccharomyces cerevisiae)
- Erad-associated e3 ubiquitin-protein ligase hrd1 (55 kDa, Protein from Saccharomyces cerevisiae)
- Degradation in the endoplasmic reticulum protein 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- U1 snp1-associating protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Erad-associated e3 ubiquitin-protein ligase component hrd3 (88 kDa, Protein from Saccharomyces cerevisiae)
CryoEM map of Hrd1/Hrd3 part from Hrd1-Usa1/Der1/Hrd3 complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6vk1
Q-score: 0.401
Wu X, Siggel M, Ovchinnikov S, Mi W, Svetlov V, Nudler E, Liao M, Hummer G, Rapoport TA
Science (2020) 368 [ DOI: doi:10.1126/science.aaz2449 Pubmed: 32327568 ]
CryoEM map of Hrd3/Yos9 complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6vk3
Q-score: 0.405
Wu X, Siggel M, Ovchinnikov S, Mi W, Svetlov V, Nudler E, Liao M, Hummer G, Rapoport TA
Science (2020) 368 [ DOI: doi:10.1126/science.aaz2449 Pubmed: 32327568 ]
- A complex of hrd1/hrd3/yos9 (Complex from Saccharomyces cerevisiae)
- Hrd3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Protein os-9 homolog (61 kDa, Protein from Saccharomyces cerevisiae)
Cdc48-Npl4 complex in the presence of ATP-gamma-S
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6chs
Q-score: 0.286
Bodnar NO, Kim KH, Ji Z, Wales TE, Svetlov V, Nudler E, Engen JR, Walz T, Rapoport TA
Nat Struct Mol Biol (2018) 25 pp. 616-622 [ DOI: doi:10.1038/s41594-018-0085-x Pubmed: 29967539 ]
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Npl4 (73 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative cell division control protein (90 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Cdc48-npl4/ufd1 complex (Cellular component from Chaetomium thermophilum)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)