CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6xas
Q-score: 0.334
Hao Z, Epshtein V, Kim KH, Proshkin S, Svetlov V, Kamarthapu V, Bharati B, Mironov A, Walz T, Nudler E
Mol Cell (2021) 81 pp. 281- [ DOI: doi:10.1016/j.molcel.2020.11.013 Pubmed: 33296676 ]
- Dna (29-mer) (8 kDa, DNA from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- E. coli rho-dependent transcription pre-termination complex (726 kDa, Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli (strain K12))
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Rna (60-mer) (19 kDa, RNA from Escherichia coli (strain K12))
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex bound with NusG
Resolution: 7.7 Å
EM Method: Single-particle
Fitted PDBs: 6xav
Q-score: 0.103
Hao Z, Epshtein V, Kim KH, Proshkin S, Svetlov V, Kamarthapu V, Bharati B, Mironov A, Walz T, Nudler E
Mol Cell (2021) 81 pp. 281- [ DOI: doi:10.1016/j.molcel.2020.11.013 Pubmed: 33296676 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna (29-mer) (9 kDa, DNA from Escherichia coli K-12)
- E. coli rho-dependent transcription pre-termination complex (726 kDa, Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli K-12)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli K-12)
Cdc48-Npl4 complex in the presence of ATP-gamma-S
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6chs
Q-score: 0.286
Bodnar NO, Kim KH, Ji Z, Wales TE, Svetlov V, Nudler E, Engen JR, Walz T, Rapoport TA
Nat Struct Mol Biol (2018) 25 pp. 616-622 [ DOI: doi:10.1038/s41594-018-0085-x Pubmed: 29967539 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cdc48-npl4/ufd1 complex (Cellular component from Chaetomium thermophilum)
- Magnesium ion (24 Da, Ligand)
- Putative cell division control protein (90 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Npl4 (73 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
