Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7u5c
Q-score: 0.33
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ Pubmed: 35578024 DOI: doi:10.1038/s41594-022-00766-y ]
- Human cst bound to dna polymerase alpha(pola1 delta n)-primase in a recruitment state (500 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Dna primase small subunit (49 kDa, Protein from Homo sapiens)
- Cst complex subunit stn1 (42 kDa, Protein from Homo sapiens)
- Cst complex subunit ctc1 (135 kDa, Protein from Homo sapiens)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Homo sapiens)
- Cst complex subunit ten1 (13 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (59 kDa, Protein from Homo sapiens)
- Canonical telomeric dna sequence (5 kDa, DNA from Homo sapiens)
- Dna polymerase alpha subunit b (66 kDa, Protein from Homo sapiens)
Human CST bound to full-length DNA polymerase alpha-primase in a recruitment state
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ Pubmed: 35578024 DOI: doi:10.1038/s41594-022-00766-y ]
- Telomeric g-strand ssdna (3 repeats) (DNA from Homo sapiens)
- Dna polymerase alpha b subunit pola2 (Protein from Homo sapiens)
- Cst complex subunit stn1 (Protein from Homo sapiens)
- Cst complex subunit ten1 (Protein from Homo sapiens)
- Cst complex subunit ctc1 (Protein from Homo sapiens)
- Dna polymerase alpha catalytic subunit pola1 (Protein from Homo sapiens)
- Dna primase large subunit prim2 (Protein from Homo sapiens)
- Dna primase catalytic subunit (Protein from Homo sapiens)
- Cst bound to full-length dna polymerase alpha-primase and ssdna (540 kDa, Complex)
Cryo-EM structure of PRC2:EZH1-AEBP2-JARID2
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7kso
Q-score: 0.379
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ Pubmed: 33514705 DOI: doi:10.1038/s41467-020-20775-z ]
- Zinc ion (65 Da, Ligand)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- Zinc finger protein aebp2 (33 kDa, Protein from Homo sapiens)
- Protein jumonji (138 kDa, Protein from Homo sapiens)
- Histone-binding protein rbbp4 (47 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh1 (85 kDa, Protein from Homo sapiens)
- Polycomb protein eed (50 kDa, Protein from Homo sapiens)
- Prc2:ezh1-aebp2-jarid2 (330 kDa, Complex from Homo sapiens)
Cryo-EM map of PRC2:EZH1-AEBP2
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ Pubmed: 33514705 DOI: doi:10.1038/s41467-020-20775-z ]
- Suz12 (Protein from Homo sapiens)
- Aebp2 (Protein from Homo sapiens)
- Ezh1 (Protein from Homo sapiens)
- Prc2:ezh1-aebp2 (299 kDa, Complex from Homo sapiens)
- Rbap48 (Protein from Homo sapiens)
- Eed (Protein from Homo sapiens)
PRC2:EZH1_A from a dimeric PRC2 bound to a nucleosome
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7ksr
Q-score: 0.287
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ Pubmed: 33514705 DOI: doi:10.1038/s41467-020-20775-z ]
- Zinc ion (65 Da, Ligand)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- Histone-binding protein rbbp4 (47 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh1 (85 kDa, Protein from Homo sapiens)
- Polycomb protein eed (50 kDa, Protein from Homo sapiens)
- Prc2:ezh1_a from a prc2:ezh1 dimer bound to a nucleosome (266 kDa, Complex from Homo sapiens)
PRC2:EZH1_B from a dimeric PRC2 bound to a nucleosome
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7ktp
Q-score: 0.207
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ Pubmed: 33514705 DOI: doi:10.1038/s41467-020-20775-z ]
- Zinc ion (65 Da, Ligand)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- Histone-binding protein rbbp4 (47 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh1 (85 kDa, Protein from Homo sapiens)
- Polycomb protein eed (50 kDa, Protein from Homo sapiens)
- Prc2:ezh1_a from a prc2:ezh1 dimer bound to a nucleosome (266 kDa, Complex from Homo sapiens)
Nucleosome from a dimeric PRC2 bound to a nucleosome
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ktq
Q-score: 0.494
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ Pubmed: 33514705 DOI: doi:10.1038/s41467-020-20775-z ]
- Histone h4 (8 kDa, Protein from Xenopus laevis)
- Histone h2b (10 kDa, Protein from Xenopus laevis)
- 601 dna (167-mer) (51 kDa, DNA from Homo sapiens)
- Nucleosome from a prc2:ezh1 dimer bound to a nucleosome (220 kDa, Complex from Xenopus laevis)
- Histone h3 (11 kDa, Protein from Xenopus laevis)
- 601 dna (167-mer) (51 kDa, DNA from Homo sapiens)
- Histone h2a (11 kDa, Protein from Xenopus laevis)
PRC2:EZH1 dimer bound to a nucleosome (composite map)
Grau D, Zhang Y, Lee CH, Valencia-Sanchez M, Zhang J, Wang M, Holder M, Svetlov V, Tan D, Nudler E, Reinberg D, Walz T, Armache KJ
Nat Commun (2021) 12 pp. 714-714 [ Pubmed: 33514705 DOI: doi:10.1038/s41467-020-20775-z ]
- Suz12 (Protein from Homo sapiens)
- Histone h2b (Protein from Xenopus laevis)
- Aebp2 (Protein from Homo sapiens)
- Ezh1 (Protein from Homo sapiens)
- Rbap48 (Protein from Homo sapiens)
- 167bp dna 601 strand (DNA from Homo sapiens)
- Eed (Protein from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Prc2:ezh1_a from a prc2:ezh1 dimer bound to a nucleosome (266 kDa, Complex from Homo sapiens)
- Histone h3 (Protein from Xenopus laevis)
- Histone h2a (Protein from Xenopus laevis)
- Jarid2 (Protein from Homo sapiens)
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6xas
Q-score: 0.334
Hao Z, Epshtein V, Kim KH, Proshkin S, Svetlov V, Kamarthapu V, Bharati B, Mironov A, Walz T, Nudler E
Mol Cell (2021) 81 pp. 281- [ DOI: doi:10.1016/j.molcel.2020.11.013 Pubmed: 33296676 ]
- Dna (29-mer) (8 kDa, DNA from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- E. coli rho-dependent transcription pre-termination complex (726 kDa, Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli (strain K12))
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Rna (60-mer) (19 kDa, RNA from Escherichia coli (strain K12))
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex bound with NusG
Resolution: 7.7 Å
EM Method: Single-particle
Fitted PDBs: 6xav
Q-score: 0.103
Hao Z, Epshtein V, Kim KH, Proshkin S, Svetlov V, Kamarthapu V, Bharati B, Mironov A, Walz T, Nudler E
Mol Cell (2021) 81 pp. 281- [ DOI: doi:10.1016/j.molcel.2020.11.013 Pubmed: 33296676 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna (29-mer) (9 kDa, DNA from Escherichia coli K-12)
- E. coli rho-dependent transcription pre-termination complex (726 kDa, Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli K-12)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli K-12)