Cryo-EM structure of Escherichia coli AcrBZ and DARPin in Saposin A-nanodisc with cardiolipin
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 6sgr
Q-score: 0.54
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump accessory protein acrz (Complex from Shigella boydii 965-58)
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Ternary complex of acrbz and darpin in saposin a-nanodisc with cardiolipin (Complex)
- Multidrug efflux pump accessory protein acrz (5 kDa, Protein from Escherichia coli K-12)
- Darpin (Complex from synthetic construct)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K-12)
- Darpin (18 kDa, Protein from synthetic construct)
Cryo-EM structure of Escherichia coli AcrBZ and DARPin in Saposin A-nanodisc
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6sgs
Q-score: 0.425
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump accessory protein acrz (Complex from Shigella boydii 965-58)
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Multidrug efflux pump accessory protein acrz (5 kDa, Protein from Shigella boydii 965-58)
- Ternary complex of acrbz and darpin in saposin a-nanodisc (Complex)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K12)
- Darpin (Complex from synthetic construct)
- Darpin (18 kDa, Protein from synthetic construct)
Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc with cardiolipin
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 6sgt
Q-score: 0.483
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Ternary complex of acrb and darpin in saposina-nanodisc with cardiolipin (Complex)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K12)
- Darpin (Complex from synthetic construct)
- Darpin (18 kDa, Protein from synthetic construct)
Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 6sgu
Q-score: 0.477
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Ternary complex of acrb and darpin in saposina-nanodisc (Complex)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K12)
- Darpin (Complex from synthetic construct)
- Darpin (18 kDa, Protein from synthetic construct)
MinCD filament from Pseudomonas aeruginosa
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6riq
Q-score: 0.499
Szewczak-Harris A, Wagstaff J, Lowe J
FEBS Lett (2019) 593 pp. 1915-1926 [ DOI: doi:10.1002/1873-3468.13471 Pubmed: 31166018 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mincd filament (Complex from Pseudomonas aeruginosa)
- Minc (15 kDa, Protein from Pseudomonas aeruginosa)
- Site-determining protein (29 kDa, Protein from Pseudomonas aeruginosa)
AlfA from B. subtilis plasmid pLS32 filament structure at 3.4 A
Resolution: 3.44 Å
EM Method: Helical reconstruction
Fitted PDBs: 6f95
Q-score: 0.47
PNAS (2018) 115 pp. 3458-3463 [ Pubmed: 29440489 DOI: doi:10.1073/pnas.1716424115 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Alfa filament (Complex from Bacillus subtilis)
- Magnesium ion (24 Da, Ligand)
- Alfa (31 kDa, Protein from Bacillus subtilis)